FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7637619
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG153849820.143686140929525No Hit
TCGTACGACTCTTAGCGG3407874.461953391495439No Hit
TAGCAGCACGTAAATATTGGCG2830103.7054741798458393No Hit
TAGCTTATCAGACTGATGTTGAC2693863.527093980466949No Hit
AACATTCAACGCTGTCGGTGAG2623243.434630609356136No Hit
AACATTCAACGCTGTCGGTGAGT2319173.0365091529179447No Hit
CCCCCCACTGCTAAATTTGACTG2106042.757456217703449No Hit
TTCAAGTAATCCAGGATAGGCT2028612.6560764552408282No Hit
AAGCTGCCAGTTGAAGAACTGT1954462.5589912248830426No Hit
TAGCTTATCAGACTGATGTTGA1214361.589971953301153No Hit
CCCCCACTGCTAAATTTGACTG1013231.3266307209092256No Hit
TAGCTTATCAGACTGATGTTGACT834791.0929976999376376No Hit
TGAGGTAGTAGGTTGTATAGTT711740.931887280577887No Hit
TGAGAACTGAATTCCATGGGTT687740.9004638749327507No Hit
TATTGCACTTGTCCCGGCCTGT668070.8747097753894244No Hit
AGCAGCATTGTACAGGGCTATGA564010.7384631257463877No Hit
CATAAAGTAGAAAGCACTACT523160.6849778707212287No Hit
TTCACAGTGGCTAAGTTCCGC484140.6338886503765113No Hit
CCCCCACTGCTAAATTTGACTGGCTT443450.5806128847223199No Hit
TAGCTTATCAGACTGATGTTGACA427130.5592449688836272No Hit
TATTGCACTTGTCCCGGCCTGTA366300.4795997286588922No Hit
CATTGCACTTGTCTCGGTCTGA365280.478264233918974No Hit
TAGCAGCACGTAAATATTGGC343640.4499307964956094No Hit
TTCAAGTAATTCAGGATAGGTT340900.44634329101778975No Hit
CAACGGAATCCCAAAAGCAGCT339570.4446019106216217No Hit
TGTAAACATCCTTGACTGGAAGCT311080.4072997095037079No Hit
TGAGGTAGTAGATTGTATAGTT282670.37010225307127786No Hit
TTAATGCTAATCGTGATAGGGGTT267070.3496770394019393No Hit
CAACGGAATCCCAAAAGCAGCTGT258880.3389538022255365No Hit
ACTGGACTTGGAGTCAGAAGGC249250.3263451607104256No Hit
CTCCCACTGCTTCACTTGACTAGCCT238880.3127676308545896No Hit
TATTGCACTTGTCCCGGCCTGTAT235150.307883909893908No Hit
AACGGAATCCCAAAAGCAGCTG227490.29785460625883536No Hit
TGATGTAGTAGGTTGTATAGTT219660.2876027201671097No Hit
TGAGGTAGTAGTTTGTGCTGTT213000.27888272510058437No Hit
AACATTCAACGCTGTCGGTGAGTT204800.26814639483849617No Hit
TGATAACTGAATTCCATGGGTT201050.2632364877064436No Hit
TGAGAACTGAATTCCATAGGCTGT198860.2603691019413249No Hit
CAACACCAGTCGATGGGCTGT198780.26026435725584113No Hit
TGTAAACATCCTACACTCTCAGCT195050.2553806362951595No Hit
TGTAAACATCCCCGACTGGAAGCT187730.24579649757339297No Hit
CCCATAAAGTAGAAAGCACTAC185670.24309932192218545No Hit
TATTGCACTTGTCCCGGCCTGTAA179160.23457572314094224No Hit
TTCACAGTGGCTAAGTTCTGC174490.2284612521258261No Hit
TAGCAGCACGTAAATATTGGCGT172790.22623542755929563No Hit
CCCATAAAGTAGAAAGCACT156570.2049984425774577No Hit
TTCACCACCTTCTCCACCCAGC149540.19579400334056987No Hit
CGCGACCTCAGATCAGACGTGG147520.19314920003210423No Hit
TCGGACGACTCTTAGCGG145420.19039965203815482No Hit
CTCGTACGACTCTTAGCGG138880.1818367739998552No Hit
CAAAGAATTCTCCTTTTGGGCT138420.1812344920583234No Hit
CACCCGGCTGTGTGCACATGTGC127200.1665440499192222No Hit
ATCACATTGCCAGGGATTTCC125890.16482885569442518No Hit
CACCCGTAGAACCGACCTTGCG122990.1610318608456379No Hit
AGCAGCATTGTACAGGGCTATG122480.16036411347567875No Hit
AGCAGCATTGTACAGGGCTATGT117670.15406633926096602No Hit
CCCATAAAGTAGAAAGCACTA113760.1489469427579459No Hit
TATTGCACTTGTCCCGGCCTGTT113760.1489469427579459No Hit
TGAGGTAGTAGGTTGTGTGGTT107590.1408685088900088No Hit
CACTAGATTGTGAGCTCCTGGA102720.13449217616118322No Hit
CAAAGTGCTGTTCGTGCAGGTAG101470.13285554045049905No Hit
TCAGTGCATCACAGAACTTTGT100190.13117962548275844No Hit
TGAGGTAGTAGTTTGTACAGTT98720.12925494188699382No Hit
ACCACAGGGTAGAACCACGGAC94170.12329758790010342No Hit
AATCCTTGGAACCTAGGTGTGAGT91810.12020761967833168No Hit
CGACGGAATCCCAAAAGCAGCTG91150.11934347602309045No Hit
ACCCCCCACTGCTAAATTTGACTG91150.11934347602309045No Hit
CAAAGAATTCTCCTTTTGGGCTT91100.11927801059466306No Hit
TGATGTAGTAGATTGTATAGTT88820.11629278705837513No Hit
AACATTCAACGCTGTCGGTGAGA88210.11549410883156125No Hit
CCCCCCACTGCTAAATTTGACTGGCT86520.11328137735071622No Hit
ACTTGACTTGGAGTCAGAAGGC85440.1118673240966851No Hit
CCCCCCACTGCTAAATTTGACTGG84570.11072822564204893No Hit
CACTAGATTGTGAGCTCCTGGAA83340.10911777610273568No Hit
CAAAGAATTCTCCTTTTGGGC81020.10608018022370584No Hit
TGGCTCAGTTCAGCAGGAACAGT79540.10414240354225576No Hit
ACTGGACTTGGAGTCAGAAGG78210.10240102314608782No Hit
ATCCCACCGCTGCCACCA77820.10189039280435433No Hit
TTCAAGTAATCCAGGATAGGCTT77220.10110480766322594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)