FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999909
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6279808.971259483516143No Hit
CCCCCCACTGCTAAATTTGACTG5580987.972932219547426No Hit
TTCAAGTAATCCAGGATAGGCT2522003.602903980608891No Hit
AAGCTGCCAGTTGAAGAACTGT2089462.984981661904462No Hit
GCCTGTCTGAGCGTCGCT2078662.9695528899018546No Hit
TAGCAGCACGTAAATATTGGCG2066342.95195266109888No Hit
AACATTCAACGCTGTCGGTGAGT1824922.607062463240594No Hit
CCCCCACTGCTAAATTTGACTG1638742.3410875769956436No Hit
AACATTCAACGCTGTCGGTGAG1428822.0411979641449625No Hit
TAGCTTATCAGACTGATGTTGAC1356331.9376394750274613No Hit
TAGCTTATCAGACTGATGTTGACT1280221.8289094901090857No Hit
TATTGCACTTGTCCCGGCCTGTAA1144551.6350926847763305No Hit
TATTGCACTTGTCCCGGCCTGTAT1016131.451633156945326No Hit
TCCTGTCTGAGCGTCGCT901611.2880310301176772No Hit
TGAGGTAGTAGGTTGTATAGTT743181.0616995163794272No Hit
TGTAAACATCCTTGACTGGAAGCT726311.0375992030753542No Hit
TTAATGCTAATCGTGATAGGGGTT668810.955455278061472No Hit
TAGCTTATCAGACTGATGTTGA663390.9477123202601634No Hit
TGAGGTAGTAGATTGTATAGTT480090.685851773215909No Hit
TATTGCACTTGTCCCGGCCTGT464810.6640229180122198No Hit
AGCAGCATTGTACAGGGCTATGA441330.6304796248065511No Hit
TTCAAGTAATTCAGGATAGGTT426400.6091507761029464No Hit
CAACGGAATCCCAAAAGCAGCTGT421590.6022792582017852No Hit
AACCGGGCGGAAACACCA412230.5889076557995254No Hit
TATTGCACTTGTCCCGGCCTGTA407280.5818361352983303No Hit
CCCCCACTGCTAAATTTGACTGGCTT357680.5109780712863553No Hit
TGTAAACATCCCCGACTGGAAGCT337600.4822919840815073No Hit
ACTGGACTTGGAGTCAGAAGGC323480.46212029327809834No Hit
CATAAAGTAGAAAGCACTACT308160.4402342944743996No Hit
CAAAGAATTCTCCTTTTGGGCT294050.42007688957099293No Hit
CAACGGAATCCCAAAAGCAGCT292520.41789114687062356No Hit
CCCATAAAGTAGAAAGCACTAC270030.3857621577651938No Hit
TTCTCACTACTGCACTTGACTAG242780.3468330802586148No Hit
TGTAAACATCCTACACTCTCAGCT202190.2888466121488151No Hit
CTTCTCACTACTGCACTTGACTAG199380.2848322742481367No Hit
TAGCTTATCAGACTGATGTTGACA197870.28267510334777207No Hit
CCCCCCACTGCTAAATTTGACTGGCT188630.26947493174554127No Hit
CTCCCACTGCTTCACTTGACTAG181390.2591319401437933No Hit
CATTGCACTTGTCTCGGTCTGA179610.25658904994336357No Hit
TAGCAGCACGTAAATATTGGC161310.23044585293894534No Hit
TGAGGTAGTAGTTTGTACAGTT159060.22723152543840214No Hit
CAAAGAATTCTCCTTTTGGGCTT158760.22680294843832968No Hit
ACCCCCCACTGCTAAATTTGACTG157440.22491720963801104No Hit
TCGTTTCCCGGCCAATGCACCA155940.22277432463764885No Hit
AGCTCGGTCTGAGGCCCCTCAGT153410.21915999193703803No Hit
AACATTCAACGCTGTCGGTGAGTT150620.21517422583636447No Hit
TCGTACGACTCTTAGCGG149280.21325991523604093No Hit
TGAGAACTGAATTCCATAGGCTGT148850.2126456215359371No Hit
AACGGAATCCCAAAAGCAGCTG140980.20140261823403705No Hit
CCCCCCACTGCTAAATTTGACTGG137860.19694541743328378No Hit
CTCCCACTGCTTCACTTGACTAGCCT132180.18883102623191245No Hit
TTCAAGTAATCCAGGATAGGCTT131830.18833101973182795No Hit
TGAGAACTGAATTCCATGGGTT131820.1883167338318255No Hit
CACCCGTAGAACCGACCTTGCG131000.18714529003162755No Hit
GGAATACCGGGTGCTGTAGGCTTT128070.18295952133092014No Hit
TATTGCACTTGTCCCGGCCTGTAG126800.18114521203061354No Hit
CACTAGATTGTGAGCTCCTGGAA124050.1772165895299496No Hit
CCCATAAAGTAGAAAGCACT123040.17577371362970576No Hit
TGAGGTAGTAGTTTGTGCTGTT121690.1738451171293798No Hit
TTCACAGTGGCTAAGTTCCG119100.1701450690287545No Hit
CACTAGATTGTGAGCTCCTGGA118920.16988792282871104No Hit
CTACGCCTGTCTGAGCGTCGCT107180.15311627622587665No Hit
TATTGCACTTGTCCCGGCCTGTAAT106600.1522876940257366No Hit
CGCCTGTCTGAGCGTCGCT105880.1512591092255628No Hit
TATTGCACTTGTCCCGGCCTGTT104510.14930194092523202No Hit
TTCAAGTAATCCAGGATAGGC99290.14184470112397174No Hit
TTCACAGTGGCTAAGTTCTGC98890.14127326512387517No Hit
CAACGGAATCCCAAAAGCAGCTGA97780.1396875302236072No Hit
CACCCGGCTGTGTGCACATGTGC96030.13718749772318467No Hit
TATTGCACTTGTCCCGGCCTGTAAA93820.13403031382265113No Hit
AATGCACCCGGGCAAGGATTCT91630.13090170172212237No Hit
CCCCCCACTGCTAAATTTGACTGTA90540.12934453862185924No Hit
ATGCACCTGGGCAAGGATTCTGA89770.12824452432167333No Hit
CAACACCAGTCGATGGGCTGT89580.12797309222162745No Hit
TTAATGCTAATCGTGATAGGGGTTT87740.12534448662118322No Hit
TGTAAACATCCCCGACTGGAAGC85760.12251587842070517No Hit
ATCACATTGCCAGGGATTTCCA83960.11994441642027062No Hit
ATCACATTGCCAGGGATTTCC83240.11891583162009678No Hit
TATTGCACTTGTCCCGGCCTGTTT81600.11657294401970084No Hit
CGCGACCTCAGATCAGACGTGGCG79290.11327290111914311No Hit
TTCACAGTGGCTAAGTTCTG78760.11251574841901517No Hit
TGTCAGTTTGTCAAATACCCCAA76420.10917284781845021No Hit
TTATAAAGCAATGAGACTGATT74560.10651567041800114No Hit
CAAAGTGCTGTTCGTGCAGGTAG74500.10642995501798666No Hit
TCGTTTCCCGGGCGGCGCACCA74390.10627281011796011No Hit
TGAGGTAGTAGGTTGTATAGT74310.1061585229179408No Hit
GGAATACCGGGTGCTGTAGGCTT73380.10482993421771626No Hit
AGCAGCATTGTACAGGGCTATGT73310.10472993291769936No Hit
GGGAATACCGGGTGCTGTAGGCTTT72510.1035870609175062No Hit
CCCCCACTGCTAAATTTGACTGGCT72360.10337277241746999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)