FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7114596
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT156889922.051835409909433No Hit
CAACGGAATCCCAAAAGCAGCTG4569976.423372458534539No Hit
CCCCCCACTGCTAAATTTGACTG2651753.727196878079936No Hit
TTCAAGTAATCCAGGATAGGCT2186873.073779593388015No Hit
AAGCTGCCAGTTGAAGAACTGT1892632.660207269674905No Hit
TAGCAGCACGTAAATATTGGCG1848892.598728023348058No Hit
AACATTCAACGCTGTCGGTGAGT1600942.2502191269890797No Hit
AACATTCAACGCTGTCGGTGAG1302761.8311088921985168No Hit
CCCCCACTGCTAAATTTGACTG1182481.6620479926056235No Hit
TGAGGTAGTAGGTTGTATAGTT972471.3668660876879024No Hit
CATAAAGTAGAAAGCACTACT798261.1220032732708927No Hit
TCGTACGACTCTTAGCGG670440.9423444423267322No Hit
TAGCTTATCAGACTGATGTTGA648100.9109442054053386No Hit
TCCTGTCTGAGCGTCGCT630130.8856862708718808No Hit
TAGCTTATCAGACTGATGTTGAC602980.8475252846401959No Hit
TATTGCACTTGTCCCGGCCTGT595190.8365759629921362No Hit
TAGCTTATCAGACTGATGTTGACT569870.8009871537329738No Hit
TGAGGTAGTAGATTGTATAGTT556520.7822229118842448No Hit
TATTGCACTTGTCCCGGCCTGTAA548400.7708097550444185No Hit
TATTGCACTTGTCCCGGCCTGTAT510980.7182136554204904No Hit
TTCAAGTAATTCAGGATAGGTT419560.589717251689344No Hit
CGCCTGTCTGAGCGTCGCT418590.5883538573377884No Hit
TGTAAACATCCTTGACTGGAAGCT370020.5200857504769069No Hit
TATTGCACTTGTCCCGGCCTGTA300800.4227928051009502No Hit
AGCAGCATTGTACAGGGCTATGA292940.41174509416978844No Hit
CCCATAAAGTAGAAAGCACTAC262750.3693112019291046No Hit
CAACGGAATCCCAAAAGCAGCTGT260210.36574107651369103No Hit
CAACACCAGTCGATGGGCTGT260120.36561457600684566No Hit
CCCATAAAGTAGAAAGCACT247770.3482558953452874No Hit
ATCCCGGACGAGCCCCCA246500.34647083263758055No Hit
CATTGCACTTGTCTCGGTCTGA244730.3439829893362884No Hit
TGAGGTAGTAGTTTGTACAGTT233590.32832503771120664No Hit
ACTGGACTTGGAGTCAGAAGGC229710.32287146030498426No Hit
TTCTCACTACTGCACTTGACTAG225660.3171789374969429No Hit
AACCGGGCGGAAACACCA220360.3097294632049381No Hit
ACCGGGTGCTGTAGGCTT194530.27342381774031865No Hit
TGTAAACATCCCCGACTGGAAGCT186640.2623339399735417No Hit
TTCACAGTGGCTAAGTTCCG185800.2611532685763183No Hit
CTCCCACTGCTTCACTTGACTAG185780.2611251573525749No Hit
TGAGGTAGTAGTTTGTGCTGTT159260.22384967466880765No Hit
CAACGGAATCCCAAAAGCAGCT149980.21080606685186343No Hit
TTCACAGTGGCTAAGTTCCGC139620.19624445295277482No Hit
CCCCCACTGCTAAATTTGACTGGCTT136910.1924353821355422No Hit
CTTCTCACTACTGCACTTGACTAG134720.18935720313563834No Hit
AACGGAATCCCAAAAGCAGCTG133470.18760025165167496No Hit
TAGCAGCACGTAAATATTGGC131060.18421284919059355No Hit
TGAGGTAGTAGGTTGTATAGT129600.1821607298573243No Hit
ACCGGGTGCTGTAGGCTTT127330.17897010596244678No Hit
GCCGGTCTGAGCGTCGCT125900.17696015346479266No Hit
GCCTGTCTGAGCGTCGCG122630.17236396838274443No Hit
TTCAAGTAATCCAGGATAGGC119670.16820350726871913No Hit
TGTAAACATCCTACACTCTCAGCT106610.14984687816426964No Hit
TCGTTTCCCGGCCAATGCACCA104900.1474433685342077No Hit
TTCACAGTGGCTAAGTTCTG103450.14540530481281017No Hit
AGCTCGGTCTGAGGCCCCTCAGT102450.14399974362563947No Hit
CTACGCCTGTCTGAGCGTCGCT101890.14321262936082385No Hit
AACATTCAACGCTGTCGGTGAGTT101780.1430580176302351No Hit
TGATGTAGTAGGTTGTATAGTT101380.14249579315536678No Hit
ACCGGGTGCTGTAGGCTTA98580.1385602218312888No Hit
TTATAAAGCAATGAGACTGATT95480.13420298215105958No Hit
TTCACAGTGGCTAAGTTCTGC94090.1322492521008923No Hit
GCCTGTCTGAGCGTCGCA93910.1319962510872016No Hit
TATTGCACTTGTCCCGGCCTGTT92490.13000035420141917No Hit
TGAGGTAGTAGGTTGTGTGGTT91340.12838395883617285No Hit
CCCCCCACTGCTAAATTTGACTGGCT88970.12505277882257826No Hit
ACGCCTGTCTGAGCGTCGCT87640.1231833824436412No Hit
TTCAAGTAATCCAGGATAGGCTT85710.12047064935240175No Hit
TACGCCTGTCTGAGCGTCGCT85550.12024575956245444No Hit
CCCTGTCTGAGCGTCGCT83890.11791252799175105No Hit
ATCCCACCGCTGCCACCA82670.11619774334340278No Hit
CCCCCCACTGCTAAATTTGACTGG81870.11507329439366622No Hit
CAAAGAATTCTCCTTTTGGGCT79500.1117421143800716No Hit
AGAGGTAGTAGGTTGCATAGTT79460.1116858919325848No Hit
CACCCGGCTGTGTGCACATGTGC78900.11089877766776919No Hit
CAAAGTGCTGTTCGTGCAGGT77300.10864987976829604No Hit
TGAGGTAGTAGATTGTATAGT74500.10471430844421806No Hit
CCCATAAAGTAGAAAGCACTA73580.10342119215202099No Hit
GGAATACCGGGTGCTGTAGGCTTT72230.10152368454934055No Hit
AATGACACGATCACTCCCGTTG71310.10023056825714349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)