FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9239807
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG128486713.905777469161423No Hit
CAACGGAATCCCAAAAGCAGCTG8379659.069074711192561No Hit
TATTGCACTTGTCCCGGCCTGTAT2666992.8864131036503253No Hit
TAGCAGCACGTAAATATTGGCG2533692.742146021015374No Hit
TATTGCACTTGTCCCGGCCTGTAA2439902.64063957180058No Hit
CCCCCACTGCTAAATTTGACTG2383952.580086358946675No Hit
AACATTCAACGCTGTCGGTGAGT2381452.5773806747262142No Hit
TAGCTTATCAGACTGATGTTGACT2279572.4671186313740106No Hit
TTCAAGTAATCCAGGATAGGCT2171602.3502655412607645No Hit
AACATTCAACGCTGTCGGTGAG1943192.103063408142616No Hit
AAGCTGCCAGTTGAAGAACTGT1805331.9538611574895448No Hit
TAGCTTATCAGACTGATGTTGAC1633481.7678724241750938No Hit
TAGCTTATCAGACTGATGTTGA1343641.454186218391791No Hit
TATTGCACTTGTCCCGGCCTGTA1027141.1116465960814983No Hit
TATTGCACTTGTCCCGGCCTGT961581.0406927330841433No Hit
GGAATACCGGGTGCTGTAGGCTTT934441.0113198251868247No Hit
GCCTGTCTGAGCGTCGCT758500.8209045924877003No Hit
TGTAAACATCCTTGACTGGAAGCT745470.8068025663306604No Hit
AGCAGCATTGTACAGGGCTATGA663980.7186080834805316No Hit
TGTAAACATCCCCGACTGGAAGCT652320.7059887722763041No Hit
TGAGGTAGTAGGTTGTATAGTT640070.6927309195960478No Hit
TTCAAGTAATTCAGGATAGGTT551300.5966574842959382No Hit
GGAATACCGGGTGCTGTAGGCTT548290.5933998404945039No Hit
TGAATACCGGGTGCTGTAGGCTTT535550.5796116737070374No Hit
CAACGGAATCCCAAAAGCAGCTGT485190.5251083707700821No Hit
TGAGGTAGTAGATTGTATAGTT473360.5123050730388633No Hit
TCCTGTCTGAGCGTCGCT363970.39391515428839585No Hit
TATTGCACTTGTCCCGGCCTGTAG348920.37762693528122393No Hit
ACTGGACTTGGAGTCAGAAGGC346460.3749645420082909No Hit
CACTAGATTGTGAGCTCCTGGAA327010.3539143187731086No Hit
TGAATACCGGGTGCTGTAGGCTT313090.3388490690335848No Hit
TAGCTTATCAGACTGATGTTGACA312540.3382538185050835No Hit
CATAAAGTAGAAAGCACTACT298390.32293964581727735No Hit
CCCCCCACTGCTAAATTTGACTGGCT298160.32269072286899503No Hit
CAAAGAATTCTCCTTTTGGGCT291040.3149849342091236No Hit
CTTCTCACTACTGCACTTGACTAG278880.301824486160804No Hit
ACCCCCCACTGCTAAATTTGACTG278830.3017703724763948No Hit
CCCCCACTGCTAAATTTGACTGGCTT266180.28807961032086493No Hit
AGCTCGGTCTGAGGCCCCTCAGT265310.2871380322121447No Hit
TATTGCACTTGTCCCGGCCTGTAAT260290.2817050182974601No Hit
CCCATAAAGTAGAAAGCACTAC258290.27954047092109174No Hit
TATTGCACTTGTCCCGGCCTGTTT253900.2747892894299632No Hit
CACTAGATTGTGAGCTCCTGGA253670.27454036648168084No Hit
CATTGCACTTGTCTCGGTCTGA251390.2720727824726209No Hit
TATTGCACTTGTCCCGGCCTGTT250380.2709796860475549No Hit
CTCCCACTGCTTCACTTGACTAG248210.26863115214419525No Hit
CACCCGGCTGTGTGCACATGTGC246460.2667371731898729No Hit
CAACGGAATCCCAAAAGCAGCT245830.2660553407663169No Hit
TGTAAACATCCTACACTCTCAGCT239090.2587608161079555No Hit
CCCCCCACTGCTAAATTTGACTGG238640.25827379294827263No Hit
TTCTCACTACTGCACTTGACTAG217910.2358382593922146No Hit
TGATGTAGTAGGTTGTATAGTT213020.23054594105699394No Hit
TATTGCACTTGTCCCGGCCTGTAAA210810.22815411620610693No Hit
TGTAAACATCCCCGACTGGAAGC178810.19352135818421318No Hit
CAAAGAATTCTCCTTTTGGGCTT176450.19096719228009848No Hit
TAGCAGCACGTAAATATTGGC175360.18978751395997773No Hit
CCCATAAAGTAGAAAGCACT168240.18208172530010638No Hit
TGAGGTAGTAGTTTGTACAGTT167710.18150812024536875No Hit
AACATTCAACGCTGTCGGTGAGTT164880.17844528570780754No Hit
GGAATACCGGGTGCTGTAGGCTTA160330.17352094042656951No Hit
TCGTACGACTCTTAGCGG159310.17241702126462166No Hit
TGATGTAGTAGATTGTATAGTT155370.168152862933176No Hit
CAACACCAGTCGATGGGCTGT151310.1637588317591482No Hit
AGCAGCATTGTACAGGGCTATGT145160.15710284857681553No Hit
CCCCCCACTGCTAAATTTGACTGA139300.15076072476405622No Hit
ACTGGACTTGGAGTCAGAAGGCA139260.15071743381652886No Hit
AACGGAATCCCAAAAGCAGCTG137500.1488126321253247No Hit
TGAGAACTGAATTCCATAGGCTGT136540.1477736493846679No Hit
TTCAAGTAATCCAGGATAGGCTT135860.14703770327670265No Hit
AACCGGGCGGAAACACCA130540.1412800072555628No Hit
CTCCCACTGCTTCACTTGACTAGCCT128450.13901805524725788No Hit
TATTGCACTTGTCCCGGCCTGTATT128090.13862843671951156No Hit
ACTTGACTTGGAGTCAGAAGGC124550.13479718786333958No Hit
ACTGGACTTGGAGTCAGAAGGCT122290.13235124932804332No Hit
TCGTTTCCCGGGCGGCGCACCA120690.13061961142694864No Hit
ATCCCGGACGAGCCCCCA118290.1280221545753066No Hit
TATTGCACTTGTCCCGGCCTGA111600.12078174360135444No Hit
CCCCCCACTGCTAAATTTGACTGT110760.11987263370327973No Hit
TATTGCACTTGTCCCGGCCTGTTA107880.11675568548130928No Hit
TATGGCACTTGTCCCGGCCTGTAT106900.11569505726688879No Hit
CCCCCCACTGCTAAATTTGAC100130.10836806439788191No Hit
GGAATACCGGGTGCTGTAGGCT98660.10677712207625117No Hit
TGAGGTAGTAGTTTGTGCTGTT97520.10554333007172119No Hit
TAGCTTATCAGACTGATGTTGACC96470.10440694269912781No Hit
TATGGCACTTGTCCCGGCCTGTAA96360.10428789259342755No Hit
CCCGCCACTGCTAAATTTGACTG96280.10420131069837281No Hit
GCCCCCACTGCTAAATTTGACTG95900.10379004669686283No Hit
CTACGGGGATGATTTTACGAACTGAA94680.10246967279727813No Hit
CAACGGAATCCCAAAAGCAGCTGA94140.10188524500565867No Hit
ATGCACCTGGGCAAGGATTCTGA94020.10175537216307656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)