FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14635856
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG171092411.689948302306336No Hit
CAACGGAATCCCAAAAGCAGCTG11487537.848895206402687No Hit
AACATTCAACGCTGTCGGTGAGT6250544.270703401290638No Hit
TAGCAGCACGTAAATATTGGCG4223122.8854615678099047No Hit
AAGCTGCCAGTTGAAGAACTGT4149222.8349691333393823No Hit
TTCAAGTAATCCAGGATAGGCT3785852.586695305009833No Hit
TAGCTTATCAGACTGATGTTGACT3685092.517850681231081No Hit
TAGCTTATCAGACTGATGTTGAC3556442.429950117027661No Hit
CCCCCACTGCTAAATTTGACTG3290212.248047534766672No Hit
AACATTCAACGCTGTCGGTGAG2793681.9087916688986282No Hit
TATTGCACTTGTCCCGGCCTGTAA2677171.829185802320001No Hit
TAGCTTATCAGACTGATGTTGA2294201.5675202051728305No Hit
TATTGCACTTGTCCCGGCCTGTAT2188281.4951499932767853No Hit
TGTAAACATCCTTGACTGGAAGCT1257750.8593621035899779No Hit
AGCAGCATTGTACAGGGCTATGA1078510.7368957442598506No Hit
TATTGCACTTGTCCCGGCCTGTA913200.62394710633939No Hit
TGTAAACATCCCCGACTGGAAGCT912320.6233458432496193No Hit
TATTGCACTTGTCCCGGCCTGT902120.6163766574363673No Hit
GGAATACCGGGTGCTGTAGGCTTT839070.573297523561314No Hit
CAACGGAATCCCAAAAGCAGCTGT771120.5268704474818555No Hit
TGAGGTAGTAGGTTGTATAGTT710660.4855609402005595No Hit
ACTGGACTTGGAGTCAGAAGGC670470.4581009815893242No Hit
TTCAAGTAATTCAGGATAGGTT607430.4150286802493821No Hit
TTAATGCTAATCGTGATAGGGGTT574550.39256330480431073No Hit
TAGCTTATCAGACTGATGTTGACA557750.3810846458177779No Hit
CCCCCACTGCTAAATTTGACTGGCTT554590.378925564722692No Hit
AACATTCAACGCTGTCGGTGAGTT546540.37342537395831166No Hit
CCCATAAAGTAGAAAGCACTAC510380.34871892699682205No Hit
CCCCCCACTGCTAAATTTGACTGGCT508740.3475983912386129No Hit
GGAATACCGGGTGCTGTAGGCTT502030.3430137601791108No Hit
CATAAAGTAGAAAGCACTACT475950.32519450860954086No Hit
TGAATACCGGGTGCTGTAGGCTTT452970.3094933429243906No Hit
CGCCCCACTGCTAAATTTGACTG449960.30743674985597014No Hit
CTTCTCACTACTGCACTTGACTAG440510.3009800041760454No Hit
TGTAAACATCCTACACTCTCAGCT430210.29394249301168307No Hit
TGAGGTAGTAGATTGTATAGTT421200.28778637887664377No Hit
CAAAGAATTCTCCTTTTGGGCT411880.2814184561531625No Hit
TTTAAACATCCTTGACTGGAAGCT389410.26606574975867486No Hit
CAACGGAATCCCAAAAGCAGCT388860.26568996032756814No Hit
CCCCCCACTGCTAAATTTGACTGG377540.25795553058188053No Hit
TTCTCACTACTGCACTTGACTAG329180.22491339078493255No Hit
CAAAGAATTCTCCTTTTGGGCTT324510.2217225968880809No Hit
ACTTGACTTGGAGTCAGAAGGC322730.2205064056383173No Hit
CCCGCCACTGCTAAATTTGACTG318550.21765040596190616No Hit
TATTGCACTTGTCCCGGCCTGTAG313250.21402916235305952No Hit
ATCAGCATTGTACAGGGCTATGA308830.21100918183398362No Hit
ACCCCCCACTGCTAAATTTGACTG302940.2069848186535861No Hit
TTCAAGTAATCCAGGATAGGCTT297340.2031585989914085No Hit
GCCTGTCTGAGCGTCGCT286440.19571113572038426No Hit
CTCCCACTGCTTCACTTGACTAG284330.1942694708119566No Hit
TTTAAACATCCCCGACTGGAAGCT281220.19214455239242584No Hit
TGAATACCGGGTGCTGTAGGCTT264760.1808981995996681No Hit
CATTGCACTTGTCTCGGTCTGA263880.18029693650989734No Hit
CCCTCCACTGCTAAATTTGACTG256640.1753501810895106No Hit
TATTGCACTTGTCCCGGCCTGTAAT250850.1713941432602234No Hit
TGTCAGTTTGTCAAATACCCCAA248630.1698773204655744No Hit
CACTAGATTGTGAGCTCCTGGAA241210.16480757941318908No Hit
TAGCAGCACGTAAATATTGGC230840.1577222405030495No Hit
CTCCCACTGCTTCACTTGACTAGCCT229760.1569843267110581No Hit
CCCATAAAGTAGAAAGCACT226090.15447678632530956No Hit
TGATGTAGTAGGTTGTATAGTT225630.15416248971020213No Hit
TGTAAACATCCCCGACTGGAAGC224250.1532195998648798No Hit
CACCCGGCTGTGTGCACATGTGC220470.1506369015929099No Hit
ACTGGACTTGGAGTCAGAAGGCA211160.14427581140453966No Hit
ATCACATTGCCAGGGATTTCCA207890.14204157242323237No Hit
CACTAGATTGTGAGCTCCTGGA201520.13768924755750536No Hit
AGCAGCATTGTACAGGGCTATGT200220.13680101799307126No Hit
TATTGCACTTGTCCCGGCCTGTAAA199930.13660287447485137No Hit
TATTGCACTTGTCCCGGCCTGTT193910.13248968833801045No Hit
AGCTCGGTCTGAGGCCCCTCAGT191320.1307200617442533No Hit
TGAGGTAGTAGTTTGTACAGTT187820.12832867445539228No Hit
CGCCTGTCTGAGCGTCGCT184540.12608760293897397No Hit
AACATTCAACGCTGTCGGTGAGA184480.1260466077283078No Hit
GCCCCCACTGCTAAATTTGACTG181710.1241539955025521No Hit
CAACACCAGTCGATGGGCTGT178940.12226138327679638No Hit
TAGCTTATCAGACTGATGTTGACC172730.11801837897284587No Hit
ACTGGACTTGGAGTCAGAAGGCT167360.11434930761822198No Hit
TGCAAGTAATCCAGGATAGGCT165870.1133312598866783No Hit
TAGCAGCACGTAAATATTGGCGT164040.11208090596135954No Hit
CCCCCCACTGCTAAATTTGACTGTA163800.11191692511869479No Hit
TTAATACCGGGTGCTGTAGGCTTT163440.11167095385469768No Hit
CCCCCACTGCTAAATTTGACTGGCTA163330.11159579596847632No Hit
TATTGCACTTGTCCCGGCCTGTTT162200.11082371950092977No Hit
CAAGGGAATCCCAAAAGCAGCTG161710.11048892528048922No Hit
TCCTGTCTGAGCGTCGCT161330.11022928894627003No Hit
CTCCCCACTGCTAAATTTGACTG161260.11018146120049281No Hit
CTACGCCTGTCTGAGCGTCGCT161050.11003797796316116No Hit
AACGGAATCCCAAAAGCAGCTG160850.10990132726094054No Hit
TATGGCACTTGTCCCGGCCTGTAA160640.10975784402360887No Hit
CAATGGAATCCCAAAAGCAGCTG157440.1075714327880788No Hit
ACCACAGGGTAGAACCACGGAC152870.10444896424233745No Hit
CCCCCACTGCTAAATTTGACTGGCT152430.10414833269745208No Hit
GTAATACCGGGTGCTGTAGGCTTT152180.10397751931967628No Hit
TCGTTTCCCGGCCAATGCACCA150390.10275449553480165No Hit
TGTCAGTTTGTCAAATACCCCA150140.10258368215702587No Hit
CTACGGGGATGATTTTACGAACTGAA149240.10196875399703303No Hit
ATCACATTGCCAGGGATTTCC147180.10056125176416057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)