FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341276
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG77222410.518934310602134No Hit
CAACGGAATCCCAAAAGCAGCTG7332759.988386215148429No Hit
AACATTCAACGCTGTCGGTGAGT2918953.976079907634585No Hit
TTCAAGTAATCCAGGATAGGCT2779083.7855544458483785No Hit
AAGCTGCCAGTTGAAGAACTGT2533523.4510621859197235No Hit
TAGCAGCACGTAAATATTGGCG2239943.0511589538385424No Hit
CCCCCACTGCTAAATTTGACTG1912142.6046425716728265No Hit
AACATTCAACGCTGTCGGTGAG1803362.456466695980372No Hit
TATTGCACTTGTCCCGGCCTGTAA1670432.2753946316689357No Hit
TATTGCACTTGTCCCGGCCTGTAT1412361.9238617373873426No Hit
TGAGGTAGTAGGTTGTATAGTT1113341.5165483493605199No Hit
TATTGCACTTGTCCCGGCCTGT959991.3076609570325377No Hit
TAGCTTATCAGACTGATGTTGA940311.2808536281703615No Hit
TAGCTTATCAGACTGATGTTGACT940181.2806765472378372No Hit
TAGCTTATCAGACTGATGTTGAC812781.1071372333637912No Hit
GGAATACCGGGTGCTGTAGGCTTT678150.9237494953193423No Hit
TGAGGTAGTAGATTGTATAGTT650810.8865080130484128No Hit
TATTGCACTTGTCCCGGCCTGTA565880.7708196776691136No Hit
GGAATACCGGGTGCTGTAGGCTT465860.634576332506774No Hit
TGTAAACATCCTTGACTGGAAGCT459740.6262399070679266No Hit
TTCAAGTAATTCAGGATAGGTT457190.6227663964684068No Hit
CAACGGAATCCCAAAAGCAGCTGT448460.6108747307688746No Hit
ACTGGACTTGGAGTCAGAAGGC410460.559112612030933No Hit
AGCAGCATTGTACAGGGCTATGA406080.5531463467658756No Hit
TTAATGCTAATCGTGATAGGGGTT384590.5238735064585502No Hit
TGTAAACATCCCCGACTGGAAGCT377370.5140387038983414No Hit
CATAAAGTAGAAAGCACTACT298990.40727252319623997No Hit
GGGAATACCGGGTGCTGTAGGCTTT297980.4058967405666263No Hit
TGAGGTAGTAGTTTGTACAGTT269140.36661201676656757No Hit
TGAGAACTGAATTCCATGGGTT267920.3649501803228758No Hit
CATTGCACTTGTCTCGGTCTGA251750.34292403663886223No Hit
CAACGGAATCCCAAAAGCAGCT237610.32366307982427034No Hit
TTCTCACTACTGCACTTGACTAG228050.3106408204786198No Hit
CCCATAAAGTAGAAAGCACTAC226450.30846136284754855No Hit
GGGAATACCGGGTGCTGTAGGCTT212990.29012667552616195No Hit
GAATACCGGGTGCTGTAGGCTTT210210.2863398678921757No Hit
CACCCGGCTGTGTGCACATGTGC208690.28426938314265804No Hit
CTTCTCACTACTGCACTTGACTAG205470.2798832246601272No Hit
AACCGGGCGGAAACACCA201910.27503393143099375No Hit
AACGGAATCCCAAAAGCAGCTG200320.27286809541011675No Hit
AGCTCGGTCTGAGGCCCCTCAGT196220.26728323523049674No Hit
CTCCCACTGCTTCACTTGACTAG192650.262420320391169No Hit
TGAGGTAGTAGTTTGTGCTGTT188290.25648129834649996No Hit
TATTGCACTTGTCCCGGCCTGTAG182840.24905752079066365No Hit
CCCATAAAGTAGAAAGCACT179170.24405838984939404No Hit
TCGTACGACTCTTAGCGG177120.241265959759584No Hit
CAACACCAGTCGATGGGCTGT170990.2329159127105424No Hit
TGAGAACTGAATTCCATAGGCTGT163560.22279505633625543No Hit
ATCCCGGACGAGCCCCCA160670.21885841099013306No Hit
CACTAGATTGTGAGCTCCTGGA154600.21059009360225664No Hit
AACATTCAACGCTGTCGGTGAGTT153070.20850598724254477No Hit
TATTGCACTTGTCCCGGCCTGTT150640.20519593596535535No Hit
TGAGGTAGTAGGTTGTGTGGTT149750.20398361265807197No Hit
GCCTGTCTGAGCGTCGCT149680.20388826138671262No Hit
TGGGAATACCGGGTGCTGTAGGCTTT145270.19788113129107254No Hit
CCCCCCACTGCTAAATTTGACTGG144050.19621929484738074No Hit
CCCCCACTGCTAAATTTGACTGGCTT142390.19395810755514437No Hit
GAATACCGGGTGCTGTAGGCTT140980.19203746051776285No Hit
TGTAAACATCCTACACTCTCAGCT139250.1896809219541671No Hit
CACTAGATTGTGAGCTCCTGGAA134870.18371465668910963No Hit
ACCCCCCACTGCTAAATTTGACTG133690.1821073066861946No Hit
CGCCTGTCTGAGCGTCGCT132980.18114017236240676No Hit
CCCCCCACTGCTAAATTTGACTGGCT127960.1743021240449208No Hit
TGTAAACATCCCCGACTGGAAGC125590.17107380242889655No Hit
TAGCAGCACGTAAATATTGGC125580.17106018081870236No Hit
TTCAAGTAATCCAGGATAGGC122010.1661972659793747No Hit
TATTGCACTTGTCCCGGCCTGTAAT121860.16599294182646177No Hit
ACTGGACTTGGAGTCAGAAGGCT121510.16551618546966496No Hit
CAAAGAATTCTCCTTTTGGGCT121220.16512115877403327No Hit
TTCAAGTAATCCAGGATAGGCTT121130.16499856428228554No Hit
GACCCGCGGGCGCTCTCCAGTCCT120790.1645354295356829No Hit
ACTGGACTTGGAGTCAGAAGGCA117410.15993132529004495No Hit
TAGCTTATCAGACTGATGTTGACA114090.15540895070557217No Hit
TGAGGTAGTAGGTTGTATAGT114040.15534084265460119No Hit
GGAATACCGGGTGCTGTAGGCTTA112720.15354279010896743No Hit
TATTGCACTTGTCCCGGCCTGA109440.14907490196527143No Hit
TGGGAATACCGGGTGCTGTAGGCTT107890.1469635523851712No Hit
CAACGGAATCCCAAAAGCAGCTGA106830.14551966170458652No Hit
TATTGCACTTGTCCCGGCCTGTAAA105980.1443618248380799No Hit
TATTGCACTTGTCCCGGCCTGTTT104790.1427408532249707No Hit
TCGTTTCCCGGGCGGCGCACCA100670.13712874982496231No Hit
AACATTCAACGCTGTCGGTGA99580.13564399431379504No Hit
TCGTTTCCCGGCCAATGCACCA98530.13421372524340455No Hit
CTACGCCTGTCTGAGCGTCGCT96300.13117610617009906No Hit
ACCGGGTGCTGTAGGCTT92090.12544140827834288No Hit
CAAAGAATTCTCCTTTTGGGCTT91120.12412011208950598No Hit
ACCGGGTGCTGTAGGCTTA90600.12341178835940782No Hit
AATGACACGATCACTCCCGTTG89740.12224032988270704No Hit
AACATTCAACGCTGTCGGTGAGA86260.11750000953512714No Hit
AATGCACCCGGGCAAGGATTCT85210.11606974046473664No Hit
ACCGGGTGCTGTAGGCTTT83460.11368595868075249No Hit
ATCCCACCGCTGCCACCA79650.10849612519676416No Hit
AACATTCATTGCTGTCGGTGGGT76070.1036195887472423No Hit
CAAAGTGCTGTTCGTGCAGGT75140.10235277899918215No Hit
ACTGGACTTGGAGTCAGAAGG74540.10153548238753046No Hit
TTCACAGTGGCTAAGTTCTGC73950.10073180738607294No Hit
TTCACAGTGGCTAAGTTCCG73790.10051386162296583No Hit
AGCAGCATTGTACAGGGCTATGT73760.10047299679238322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)