FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8276725
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110153213.308790614645286No Hit
CAACGGAATCCCAAAAGCAGCTG6677258.067502544786736No Hit
CCCCCACTGCTAAATTTGACTG2804403.388296699479565No Hit
AACATTCAACGCTGTCGGTGAGT2735003.3044471092128833No Hit
GGAATACCGGGTGCTGTAGGCTTT2321612.8049862717439566No Hit
AACATTCAACGCTGTCGGTGAG2055392.4833373103492025No Hit
TATTGCACTTGTCCCGGCCTGTAA1998352.414421162959987No Hit
TTCAAGTAATCCAGGATAGGCT1618361.955314451066092No Hit
TAGCTTATCAGACTGATGTTGACT1557071.8812634224285572No Hit
TATTGCACTTGTCCCGGCCTGTAT1534141.853559227834681No Hit
GCCTGTCTGAGCGTCGCT1516561.832318942576925No Hit
TAGCAGCACGTAAATATTGGCG1472641.7792544756531117No Hit
GGAATACCGGGTGCTGTAGGCTT1386911.6756748593193564No Hit
AAGCTGCCAGTTGAAGAACTGT1376471.663061174558778No Hit
TAGCTTATCAGACTGATGTTGAC1353801.6356711138765634No Hit
TAGCTTATCAGACTGATGTTGA1252821.513666335416484No Hit
TGAGGTAGTAGGTTGTATAGTT949981.1477728207715008No Hit
TGAATACCGGGTGCTGTAGGCTTT867901.048603161274538No Hit
TATTGCACTTGTCCCGGCCTGTA752310.9089464733937638No Hit
TATTGCACTTGTCCCGGCCTGT697460.8426763001066243No Hit
TGAGGTAGTAGATTGTATAGTT527090.6368340134533889No Hit
TCCTGTCTGAGCGTCGCT525800.6352754259686048No Hit
TTAATGCTAATCGTGATAGGGGTT513190.6200399312530016No Hit
AGCAGCATTGTACAGGGCTATGA503560.6084048944479852No Hit
TGAATACCGGGTGCTGTAGGCTT503340.608139088830425No Hit
TCGTACGACTCTTAGCGG449430.5430046304546786No Hit
ACTGGACTTGGAGTCAGAAGGC421420.5091627425098696No Hit
TGTAAACATCCCCGACTGGAAGCT412860.49882048757207714No Hit
TGTAAACATCCTTGACTGGAAGCT374610.45260655633719854No Hit
GGAATACCGGGTGCTGTAGGCTTA354340.4281161933010943No Hit
CAACGGAATCCCAAAAGCAGCTGT326900.39496298354723636No Hit
CAACGGAATCCCAAAAGCAGCT317130.3831587977128635No Hit
TTCAAGTAATTCAGGATAGGTT307110.37105256004035414No Hit
CATTGCACTTGTCTCGGTCTGA290420.350887579326364No Hit
TATTGCACTTGTCCCGGCCTGTAG251810.30423869344456894No Hit
CAACACCAGTCGATGGGCTGT235550.2845932418921735No Hit
CACCCGGCTGTGTGCACATGTGC232440.28083571702575594No Hit
TAGCTTATCAGACTGATGTTGACA228400.2759545593214707No Hit
GGAATACCGGGTGCTGTAGGCT225820.27283738435190247No Hit
CACTAGATTGTGAGCTCCTGGAA218180.26360668017845224No Hit
CATAAAGTAGAAAGCACTACT213000.25734816609226474No Hit
CACTAGATTGTGAGCTCCTGGA203050.24532650293443362No Hit
TGAGAACTGAATTCCATAGGCTGT188310.22751752655790786No Hit
CCCCCACTGCTAAATTTGACTGGCTT187790.22688925873458402No Hit
TGAGGTAGTAGTTTGTACAGTT185240.22380832998559214No Hit
AACATTCAACGCTGTCGGTGAGTT182160.22008705133975093No Hit
TATTGCACTTGTCCCGGCCTGTAAT181160.21887884398720509No Hit
TATTGCACTTGTCCCGGCCTGTAAA175800.21240285257755936No Hit
ACCCCCCACTGCTAAATTTGACTG172960.20897154369632917No Hit
CAAAGAATTCTCCTTTTGGGCT156040.18852867529125347No Hit
ACTGGACTTGGAGTCAGAAGGCA151930.18356294307229007No Hit
CGCCTGTCTGAGCGTCGCT151420.1829467573224917No Hit
TCGTTTCCCGGCCAATGCACCA150510.18184728863167496No Hit
AACGGAATCCCAAAAGCAGCTG149250.1803249473674672No Hit
CCCATAAAGTAGAAAGCACT149100.18014371626458534No Hit
ACTGGACTTGGAGTCAGAAGGCT147520.1782347486475629No Hit
CCCATAAAGTAGAAAGCACTAC143490.17336567301680314No Hit
TATTGCACTTGTCCCGGCCTGTT141360.1707921913558805No Hit
TGAATACCGGGTGCTGTAGGCTTA136520.16494446776955862No Hit
TGAGGTAGTAGTTTGTGCTGTT135220.16337379821124903No Hit
TGAGAACTGAATTCCATGGGTT132280.15982166859476424No Hit
CCCCCCACTGCTAAATTTGACTGG130830.15806976793357277No Hit
TGATGTAGTAGGTTGTATAGTT128530.15529089102271731No Hit
TAGCAGCACGTAAATATTGGC117630.14212143087996762No Hit
TGTAAACATCCTACACTCTCAGCT116650.1409373876744727No Hit
ATCCCGGACGAGCCCCCA115890.14001915008653784No Hit
AGCTCGGTCTGAGGCCCCTCAGT112040.13536755177923637No Hit
TGAGGTAGTAGGTTGTGTGGTT108110.1306192968837312No Hit
AGCAGCATTGTACAGGGCTATGT107910.13037765541322202No Hit
CCCCCCACTGCTAAATTTGACTGGCT107810.13025683467796742No Hit
GCCCCCACTGCTAAATTTGACTG107170.1294835819723381No Hit
CTTCTCACTACTGCACTTGACTAG107090.12938692538413443No Hit
CAACGGAATCCCAAAAGCAGCTGA104080.12575022125297142No Hit
CCCGCCACTGCTAAATTTGACTG102570.1239258281506272No Hit
AACCGGGCGGAAACACCA102300.12359961216543984No Hit
AGCTACATCTGGCTACTGGGTCTC97100.11731693393220144No Hit
GAATACCGGGTGCTGTAGGCTTT96520.11661617366772485No Hit
TTCTCACTACTGCACTTGACTAG95520.11540796631517901No Hit
CACCCGTAGAACCGACCTTGCG92920.11226662719855982No Hit
TTCAAGTAATCCAGGATAGGCTT91860.11098592740486123No Hit
TATTGCACTTGTCCCGGCCTGTTT91120.11009185396397729No Hit
TCGTTTCCCGGGCGGCGCACCA91110.11007977189045183No Hit
CTCCCACTGCTTCACTTGACTAG88920.10743379778837645No Hit
CAAAGAATTCTCCTTTTGGGCTT88500.10692635070030719No Hit
CCCCCCACTGCTAAATTTGACTGTA88320.10670887337684895No Hit
AATGCACCCGGGCAAGGATTCT87600.10583896408301592No Hit
TTAATGCTAATCGTGATAGGGGTTT84100.10161023834910547No Hit
CCCCCCACTGCTAAATTTGACTGT83500.10088531393757796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)