FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10295487
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9264088.998195034387397No Hit
CCCCCCACTGCTAAATTTGACTG6525746.338447127367554No Hit
TAGCAGCACGTAAATATTGGCG4063233.9466127245850537No Hit
AAGCTGCCAGTTGAAGAACTGT3333533.237855576914429No Hit
TTCAAGTAATCCAGGATAGGCT3190833.0992511573274775No Hit
AACATTCAACGCTGTCGGTGAGT3002182.9160155318539083No Hit
TAGCTTATCAGACTGATGTTGAC2329232.2623796232271483No Hit
TAGCTTATCAGACTGATGTTGA1899751.8452259713406467No Hit
GCCTGTCTGAGCGTCGCT1785791.734536695544368No Hit
TAGCTTATCAGACTGATGTTGACT1777881.7268537175560514No Hit
AACATTCAACGCTGTCGGTGAG1582251.5368384225049285No Hit
CCCCCACTGCTAAATTTGACTG1489661.4469058141688684No Hit
TATTGCACTTGTCCCGGCCTGTAA1309821.2722273361133862No Hit
TATTGCACTTGTCCCGGCCTGTAT1055211.0249248044313009No Hit
TGTAAACATCCTTGACTGGAAGCT883610.8582498331550513No Hit
TCCTGTCTGAGCGTCGCT878710.853490466259634No Hit
AGCAGCATTGTACAGGGCTATGA798690.7757670909593689No Hit
TGAGGTAGTAGGTTGTATAGTT786190.7636258488792226No Hit
TATTGCACTTGTCCCGGCCTGT743780.7224330427497019No Hit
CATAAAGTAGAAAGCACTACT680790.6612508956594283No Hit
CAACGGAATCCCAAAAGCAGCTGT622600.6047309855279308No Hit
TATTGCACTTGTCCCGGCCTGTA569990.5536309258610108No Hit
GGAATACCGGGTGCTGTAGGCTTT561270.5451611953859006No Hit
CCCATAAAGTAGAAAGCACTAC558680.5426455300268943No Hit
TGTAAACATCCCCGACTGGAAGCT552480.5366234739551416No Hit
TTCAAGTAATTCAGGATAGGTT549890.5341078085961354No Hit
CCCCCACTGCTAAATTTGACTGGCTT460750.44752618307419556No Hit
TGAGGTAGTAGATTGTATAGTT454470.44142642305313No Hit
TGTAAACATCCTACACTCTCAGCT410590.3988058068549841No Hit
ACTGGACTTGGAGTCAGAAGGC394840.38350784183399966No Hit
CGCCTGTCTGAGCGTCGCT383350.37234761211392914No Hit
TAGCTTATCAGACTGATGTTGACA372670.36197413488065205No Hit
GGAATACCGGGTGCTGTAGGCTT366200.35568982797996834No Hit
CCCCCCACTGCTAAATTTGACTGGCT360190.3498523187878339No Hit
CAACGGAATCCCAAAAGCAGCT334890.32527844481761764No Hit
TTCTCACTACTGCACTTGACTAG334860.3252493058366253No Hit
TGTCAGTTTGTCAAATACCCCAA330100.3206259208525056No Hit
CTTCTCACTACTGCACTTGACTAG323870.31457472579976065No Hit
TGAATACCGGGTGCTGTAGGCTTT317540.3084264008103745No Hit
CAAAGAATTCTCCTTTTGGGCT302770.29408030916847355No Hit
CATTGCACTTGTCTCGGTCTGA282630.27451833992894165No Hit
AACATTCAACGCTGTCGGTGAGTT280100.2720609525319201No Hit
TTCACAGTGGCTAAGTTCCG260350.25287779004528876No Hit
TGATGTAGTAGGTTGTATAGTT257120.24974049309177895No Hit
AACCGGGCGGAAACACCA244230.237220444258732No Hit
CTACGCCTGTCTGAGCGTCGCT241940.23499616870964918No Hit
CTCCCACTGCTTCACTTGACTAG237940.23111097124400237No Hit
TAGCAGCACGTAAATATTGGC224130.21769732699385663No Hit
CCCATAAAGTAGAAAGCACT212850.20674107014073254No Hit
TGAATACCGGGTGCTGTAGGCTT206050.20013623444913292No Hit
TGAGGTAGTAGTTTGTACAGTT203640.19779540297608067No Hit
CCCCCCACTGCTAAATTTGACTGG201470.19568768335096728No Hit
TCGTTTCCCGGCCAATGCACCA199690.19395877047875443No Hit
TGTCAGTTTGTCAAATACCCCA199190.19347312079554857No Hit
AGCTCGGTCTGAGGCCCCTCAGT194030.18846121606486416No Hit
TGTAAACATCCCCGACTGGAAGC191710.18620780153478897No Hit
CAACACCAGTCGATGGGCTGT182330.17709701347784712No Hit
TAGCAGCACGTAAATATTGGCGT175860.17081270657716335No Hit
TGTAAACATCCTACACTCAGCT169940.16506261432800604No Hit
CAAAGAATTCTCCTTTTGGGCTT166800.1620127343174733No Hit
ATCACATTGCCAGGGATTTCCA161520.15688427366281943No Hit
TGAGGTAGTAGTTTGTGCTGTT159020.15445602524679017No Hit
ACCCCCCACTGCTAAATTTGACTG157820.15329046600709612No Hit
TTCAAGTAATCCAGGATAGGC157190.15267854740625672No Hit
TACGCCTGTCTGAGCGTCGCT157150.15263969543160028No Hit
GTCAGGATGGCCGAGCGGTCTAAGG156260.15177523899549386No Hit
TGATGTAGTAGATTGTATAGTT151980.14761807770725174No Hit
TTCAAGTAATCCAGGATAGGCTT151390.14704501108106882No Hit
AACGGAATCCCAAAAGCAGCTG147900.14365517629229196No Hit
TATTGCACTTGTCCCGGCCTGTT146090.14189712443908675No Hit
CTCCCACTGCTTCACTTGACTAGCCT145270.14110065895862914No Hit
AGCAGCATTGTACAGGGCTATGT145040.14087726010435445No Hit
TCGTACGACTCTTAGCGG141350.13729316544229525No Hit
TATTGCACTTGTCCCGGCCTGTAG140380.1363510050568759No Hit
ACTTGACTTGGAGTCAGAAGGC137490.13354394988794605No Hit
CACCCGGCTGTGTGCACATGTGC135550.13165962911710732No Hit
ATCACATTGCCAGGGATTTCC132900.1290856857961163No Hit
ACGCCTGTCTGAGCGTCGCT130930.1271722260442852No Hit
CACTAGATTGTGAGCTCCTGGAA130710.12695854018367467No Hit
CACTAGATTGTGAGCTCCTGGA122680.11915900627138862No Hit
ACTGGACTTGGAGTCAGAAGGCA120700.11723583352589344No Hit
AGCAGCATTGTACAGGGCTATG119610.11617711721650467No Hit
CCCCCACTGCTAAATTTGACTGGCT117000.11364202587017011No Hit
CAAAGTGCTGTTCGTGCAGGTAG114330.11104865656185084No Hit
CCCCCACTGCTAAATTTGACTGGCTA114130.1108543966885685No Hit
TTCACAGTGGCTAAGTTCTGC113770.11050472891666029No Hit
TTCACAGTGGCTAAGTTCCGC111710.10850385222185216No Hit
CAAAGTGCTGTTCGTGCAGGT107220.10414271806666359No Hit
ATCCCACCGCTGCCACCA106840.10377362430742712No Hit
AACATTCAACGCTGTCGGTGAGA105680.10264691704238954No Hit
ACCACAGGGTAGAACCACGGAC105120.10210298939719899No Hit
TAGCAGCACATCATGGTTTACA103840.10085972620819199No Hit
CCCATAAAGTAGAAAGCACTA103360.10039350251231438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)