FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9356492
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT192322520.554979366198356No Hit
CAACGGAATCCCAAAAGCAGCTG5011135.355778640114265No Hit
CCCCCCACTGCTAAATTTGACTG2760482.9503365150101124No Hit
TTCAAGTAATCCAGGATAGGCT2667282.8507265329783853No Hit
TAGCAGCACGTAAATATTGGCG2650202.8324718281167773No Hit
AAGCTGCCAGTTGAAGAACTGT2384552.548551316027417No Hit
AACATTCAACGCTGTCGGTGAGT1718171.836339944500567No Hit
AACATTCAACGCTGTCGGTGAG1220141.3040571188432588No Hit
TGAGGTAGTAGGTTGTATAGTT1159121.2388403688048897No Hit
CCCCCACTGCTAAATTTGACTG1132211.2100795896581753No Hit
TCCTGTCTGAGCGTCGCT1072011.1457392364574244No Hit
AACCGGGCGGAAACACCA1034991.1061731255688565No Hit
TATTGCACTTGTCCCGGCCTGTAA1008981.0783742453902594No Hit
TAGCTTATCAGACTGATGTTGAC936691.0011123827177963No Hit
TATTGCACTTGTCCCGGCCTGT919750.9830073065845619No Hit
TATTGCACTTGTCCCGGCCTGTAT905770.9680658092798028No Hit
TAGCTTATCAGACTGATGTTGACT786850.8409668922925387No Hit
TAGCTTATCAGACTGATGTTGA732990.7834025829338602No Hit
TGAGGTAGTAGATTGTATAGTT693770.7414851634565605No Hit
CATAAAGTAGAAAGCACTACT683750.7307760216115184No Hit
TGTAAACATCCTTGACTGGAAGCT553770.5918564350827211No Hit
GGAATACCGGGTGCTGTAGGCTTT541830.5790952421056952No Hit
CGCCTGTCTGAGCGTCGCT518450.554107244467264No Hit
TATTGCACTTGTCCCGGCCTGTA514140.5495008171866123No Hit
TTCAAGTAATTCAGGATAGGTT492160.5260091068319195No Hit
GGAATACCGGGTGCTGTAGGCTT436380.46639274634125694No Hit
TTCTCACTACTGCACTTGACTAG361010.3858390516445694No Hit
AGCAGCATTGTACAGGGCTATGA345460.3692195750287608No Hit
CAACGGAATCCCAAAAGCAGCTGT340410.36382225304099014No Hit
CACCCGGCTGTGTGCACATGTGC331400.3541925755935023No Hit
ACTGGACTTGGAGTCAGAAGGC304460.3253997331478507No Hit
CTACGCCTGTCTGAGCGTCGCT301670.32241784634668635No Hit
TCGTACGACTCTTAGCGG296920.3173411573482882No Hit
CATTGCACTTGTCTCGGTCTGA291550.3116018268385202No Hit
TGTAAACATCCCCGACTGGAAGCT288750.30860925227104347No Hit
TGAGGTAGTAGTTTGTACAGTT278610.29777185723025257No Hit
CCCATAAAGTAGAAAGCACTAC278150.29728021997988135No Hit
CTCCCACTGCTTCACTTGACTAG257410.2751137926479283No Hit
ACCGGGTGCTGTAGGCTT240570.25711559417781793No Hit
CTTCTCACTACTGCACTTGACTAG222780.2381020579080279No Hit
TGATGTAGTAGGTTGTATAGTT207870.22216659833621402No Hit
GCCTGTCTGAGCGTCGCTT200500.21428971456396267No Hit
ATCCCGGACGAGCCCCCA200180.2139477060419653No Hit
ACCGGGTGCTGTAGGCTTA200090.21385151614515355No Hit
CAACACCAGTCGATGGGCTGT194870.2082725021300718No Hit
CCCTGTCTGAGCGTCGCT188360.20131476626068828No Hit
TACGCCTGTCTGAGCGTCGCT186590.19942303162339045No Hit
CCCATAAAGTAGAAAGCACT182380.19492348200586287No Hit
ACCGGGTGCTGTAGGCTTT174840.18686490620630042No Hit
GCCGGTCTGAGCGTCGCT168060.1796186006464816No Hit
ACGCCTGTCTGAGCGTCGCT166110.17753448621556028No Hit
TAGCAGCACGTAAATATTGGC161970.1731097509622196No Hit
AGCTCGGTCTGAGGCCCCTCAGT159210.17015992745999248No Hit
GCCTGTCTGAGCGTCGCG156650.1674238592840137No Hit
CCCCCACTGCTAAATTTGACTGGCTT149090.15934390795182637No Hit
TGAGGTAGTAGTTTGTGCTGTT147210.15733460788509196No Hit
TGTAAACATCCTACACTCTCAGCT147160.1572811690535299No Hit
TTCAAGTAATCCAGGATAGGC141790.1515418385437619No Hit
TATTGCACTTGTCCCGGCCTGTT131960.1410357642586559No Hit
CCCCCCACTGCTAAATTTGACTGG125320.13393908742721097No Hit
AACGGAATCCCAAAAGCAGCTG125290.1339070241282737No Hit
ATCCCACCGCTGCCACCA124180.1327206820675954No Hit
TGATGTAGTAGATTGTATAGTT123590.1320901038551628No Hit
GAATACCGGGTGCTGTAGGCTTT118300.12643627547589417No Hit
TTCACAGTGGCTAAGTTCTGC117660.12575225843189947No Hit
TGTCAGTTTGTCAAATACCCCA115650.12360401740310364No Hit
TATTGCACTTGTCCCGGCCTGTAG114740.12263143066867369No Hit
TTCACAGTGGCTAAGTTCCGC113630.1214450886079954No Hit
TGTCAGTTTGTCAAATACCCCAA111800.11948922737282305No Hit
TTCAAGTAATCCAGGATAGGCTT111510.11917928214976296No Hit
AACATTCAACGCTGTCGGTGAGTT111410.11907240448663879No Hit
TGAGGTAGTAGGTTGTATAGT108710.11618670758228619No Hit
GCCTGTCTGAGCGTCGCA105890.11317275748218457No Hit
TTATAAAGCAATGAGACTGATT104710.11191160105731934No Hit
TAGCTTATCAGACTGATGTTGACA104560.11175128456263309No Hit
GGGAATACCGGGTGCTGTAGGCTTT102990.11007330525158361No Hit
GGGAATACCGGGTGCTGTAGGCTT99960.10683491205892123No Hit
CCCCCCACTGCTAAATTTGACTGGCT99270.10609745618336444No Hit
CGCGACCTCAGATCAGACGTGG99140.10595851522130302No Hit
CAACGGAATCCCAAAAGCAGCT97430.1041309071818797No Hit
TGAGGTAGTAGGTTGTGTGGTT97280.10397059068719344No Hit
GGAATACCGGGTGCTGTAGGCTTA96660.10330794917582359No Hit
CAAAGTGCTGTTCGTGCAGGT96020.1026239321318289No Hit
AGAGGTAGTAGGTTGCATAGTT94930.10145896560377543No Hit
ACCCCCCACTGCTAAATTTGACTG94710.10122383474490226No Hit
CACTAGATTGTGAGCTCCTGGA94560.101063518250216No Hit
TAGCAGCACGTAAATATTGGCGT93750.1001978091789102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)