FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7135986
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG87441912.25365352454447No Hit
TCGTACGACTCTTAGCGG85688412.007927145596978No Hit
TAGCTTATCAGACTGATGTTGAC2235833.133175989975317No Hit
AACATTCAACGCTGTCGGTGAG1474492.0662736726221156No Hit
TTCAAGTAATCCAGGATAGGCT1457252.042114432399391No Hit
AACATTCAACGCTGTCGGTGAGT1428312.0015594200997593No Hit
TAGCAGCACGTAAATATTGGCG1350281.8922122324791557No Hit
CCCCCCACTGCTAAATTTGACTG1315201.8430529432092495No Hit
TAGCTTATCAGACTGATGTTGA1193201.672088482236372No Hit
AAGCTGCCAGTTGAAGAACTGT998421.3991339108568879No Hit
CCCCCACTGCTAAATTTGACTG759551.0643939043602384No Hit
TATTGCACTTGTCCCGGCCTGT758921.0635110550945588No Hit
TGAGGTAGTAGGTTGTATAGTT727151.0189902278395726No Hit
TAGCTTATCAGACTGATGTTGACT514080.7204050007945644No Hit
AGCAGCATTGTACAGGGCTATGA511340.7165653071628784No Hit
TGAGAACTGAATTCCATGGGTT497120.6966381380232528No Hit
ACCGGGTGCTGTAGGCTT431820.6051301109615406No Hit
CATTGCACTTGTCTCGGTCTGA408680.5727029172983243No Hit
ACTGGACTTGGAGTCAGAAGGC388140.5439192285410873No Hit
TTCACAGTGGCTAAGTTCCGC374400.5246647064610273No Hit
CATAAAGTAGAAAGCACTACT367170.5145329601263231No Hit
TGAGGTAGTAGTTTGTGCTGTT356060.4989639833934652No Hit
TGAGGTAGTAGATTGTATAGTT355000.49747855447025824No Hit
TATTGCACTTGTCCCGGCCTGTA348740.48870611573509254No Hit
TAGCTTATCAGACTGATGTTGACA284990.3993701781365602No Hit
TTCAAGTAATTCAGGATAGGTT284680.39893576024392424No Hit
CTCGTACGACTCTTAGCGG257270.3605248104466573No Hit
ACCGGGTGCTGTAGGCTTT244940.34324618910407056No Hit
CAACACCAGTCGATGGGCTGT239280.3353145591933616No Hit
CGCGACCTCAGATCAGACGTGG236810.33185322953268126No Hit
TTCACCACCTTCTCCACCCAGC224360.3144064464252032No Hit
AACGGAATCCCAAAAGCAGCTG205160.2875005640425864No Hit
CACCCGGCTGTGTGCACATGTGC205130.28745852360136354No Hit
CAACGGAATCCCAAAAGCAGCT202940.28438957139209636No Hit
CTCCCACTGCTTCACTTGACTAGCCT192430.26966140348369516No Hit
TTCACAGTGGCTAAGTTCTGC191370.26817597456048825No Hit
TGTAAACATCCTTGACTGGAAGCT178610.2502947735603741No Hit
CCCCCACTGCTAAATTTGACTGGCTT178330.24990239610896098No Hit
TAGCAGCACGTAAATATTGGC173360.24293769634637735No Hit
AGCAGCATTGTACAGGGCTATG162300.22743878701555748No Hit
TCGGACGACTCTTAGCGG159400.22337487769734976No Hit
TGAGGTAGTAGGTTGTGTGGTT156640.21950715710484855No Hit
AACATTCAACGCTGTCGGTGAGTT155450.21783955293634263No Hit
TGTAAACATCCCCGACTGGAAGCT141760.1986550982583206No Hit
TATTGCACTTGTCCCGGCCTGTAT139570.19558614604905333No Hit
CCCATAAAGTAGAAAGCACT139400.19534791688212394No Hit
CTGTCACGCGGGAGACCGGGGT139260.19515172815641735No Hit
TGTAAACATCCTACACTCTCAGCT136460.19122795364228573No Hit
CAACGGAATCCCAAAAGCAGCTGT126100.17670998793999876No Hit
ACTGGACTTGGAGTCAGAAGG123000.17236580901363877No Hit
AGCAGCATTGTACAGGGCTATGT121350.17005358474638263No Hit
ATCCCACCGCTGCCACCA120320.16861019626439852No Hit
TTAATGCTAATCGTGATAGGGGTT116930.16385962640621773No Hit
CCCATAAAGTAGAAAGCACTAC116770.16363541071969592No Hit
TGAGGTAGTAGTTTGTACAGTT112350.15744145237953103No Hit
TATTGCACTTGTCCCGGCCTGTT108420.15193415457933915No Hit
TATTGCACTTGTCCCGGCCTGTAA104000.14574019623917422No Hit
TCAGTGCATCACAGAACTTTGT103130.14452102344371193No Hit
TGGCTCAGTTCAGCAGGAAC102340.14341395849151048No Hit
TGATGTAGTAGGTTGTATAGTT100910.14141003079322184No Hit
ACCACAGGGTAGAACCACGGAC99680.13968637270308545No Hit
TGAGGTAGTAAGTTGTATTGTT98460.13797672809335668No Hit
CACGCTCATGCACACACCCACA95760.1341930883833012No Hit
TAGCTTATCAGACTGATGTTG88770.12439766557837978No Hit
ATCCCGGACGAGCCCCCA88330.12378107244044481No Hit
GCGTACGACTCTTAGCGG87760.12298230405721088No Hit
TAGCAGCACGTAAATATTGGCGT85750.12016559449528068No Hit
CAAAGTGCTGTTCGTGCAGGTAG84820.11886234081737268No Hit
TCCCCGGCACCTCCACCA84700.11869417905248134No Hit
TTATAAAGCAATGAGACTGATT83560.11709664228601345No Hit
ACCGGGTGCTGTAGGCTTA80370.11262634203598493No Hit
ACTGGACTTGGAGTCAGAAGGCT80000.11210784326090326No Hit
CTTCTCACTACTGCACTTGACTAG75020.10512913001791202No Hit
CACTAGATTGTGAGCTCCTGGA74630.10458260428201512No Hit
CGCGACCTCAGATCAGACGTG73010.10231242045598184No Hit
CGCGACCTCAGATCAGACGTGGC71490.10018237143402467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)