FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891256
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4825357.002134298885427No Hit
CCCCCCACTGCTAAATTTGACTG3878035.6274647176073564No Hit
TTCAAGTAATCCAGGATAGGCT2301683.340000719752684No Hit
AAGCTGCCAGTTGAAGAACTGT2066392.9985680404268833No Hit
TAGCAGCACGTAAATATTGGCG2039772.9599393782497705No Hit
AACATTCAACGCTGTCGGTGAGT1817682.6376614074415463No Hit
GCCTGTCTGAGCGTCGCT1667552.4198056203397464No Hit
CCCCCACTGCTAAATTTGACTG1631232.3671011496307783No Hit
TGAGGTAGTAGGTTGTATAGTT1111631.61310216889345No Hit
AACATTCAACGCTGTCGGTGAG1108141.6080377800505454No Hit
TAGCTTATCAGACTGATGTTGAC1071431.5547673747717397No Hit
TAGCTTATCAGACTGATGTTGACT840521.2196905759994985No Hit
TATTGCACTTGTCCCGGCCTGTAA797671.1575103290314568No Hit
TAGCTTATCAGACTGATGTTGA760341.1033402328980377No Hit
TCCTGTCTGAGCGTCGCT735851.0678024441408067No Hit
TATTGCACTTGTCCCGGCCTGT715251.0379094899391346No Hit
TATTGCACTTGTCCCGGCCTGTAT665710.9660212884269572No Hit
AACCGGGCGGAAACACCA613580.8903747009253467No Hit
TGAGGTAGTAGATTGTATAGTT548760.796313473189793No Hit
TGTAAACATCCTTGACTGGAAGCT501790.7281546353814167No Hit
CATAAAGTAGAAAGCACTACT463330.6723447801097506No Hit
TATTGCACTTGTCCCGGCCTGTA425990.6181601728335153No Hit
TCGTACGACTCTTAGCGG407930.5919530489071949No Hit
TTCAAGTAATTCAGGATAGGTT382250.5546884341548188No Hit
ACTGGACTTGGAGTCAGAAGGC370970.5383198650579808No Hit
AGCAGCATTGTACAGGGCTATGA341900.4961359728908634No Hit
CGCCTGTCTGAGCGTCGCT330490.47957875893741286No Hit
CAACGGAATCCCAAAAGCAGCTGT307060.4455791513187146No Hit
ACCGGGTGCTGTAGGCTT304890.4424302333275676No Hit
TTCTCACTACTGCACTTGACTAG295230.4284124693669776No Hit
CACCCGGCTGTGTGCACATGTGC282350.40972211741952413No Hit
TGTAAACATCCCCGACTGGAAGCT256800.3726461475237605No Hit
CATTGCACTTGTCTCGGTCTGA233250.3384724061912662No Hit
CAACGGAATCCCAAAAGCAGCT231300.3356427333420787No Hit
CCCATAAAGTAGAAAGCACTAC224750.3261379347973722No Hit
ACCGGGTGCTGTAGGCTTT211980.3076072054208986No Hit
TTAATGCTAATCGTGATAGGGGTT199380.28932316547230286No Hit
ACCGGGTGCTGTAGGCTTA198210.2876253617627904No Hit
CCCCCACTGCTAAATTTGACTGGCTT193980.28148714835147615No Hit
TGAGGTAGTAGTTTGTACAGTT192650.27955716635690214No Hit
CTACGCCTGTCTGAGCGTCGCT183080.2656700026816592No Hit
CTTCTCACTACTGCACTTGACTAG179110.2599090789835699No Hit
CCCATAAAGTAGAAAGCACT178000.2582983421309555No Hit
CAACACCAGTCGATGGGCTGT154400.2240520450843794No Hit
CTCCCACTGCTTCACTTGACTAG154110.22363122194270535No Hit
TACGCCTGTCTGAGCGTCGCT153730.2230797985156842No Hit
AGCTCGGTCTGAGGCCCCTCAGT153520.22277506451654094No Hit
GGAATACCGGGTGCTGTAGGCTTT149610.21710120767534977No Hit
TGTAAACATCCTACACTCTCAGCT145740.2114853954054239No Hit
TGAGGTAGTAGTTTGTGCTGTT139010.20171939629002317No Hit
AACGGAATCCCAAAAGCAGCTG136730.19841085572789635No Hit
AACATTCAACGCTGTCGGTGAGTT134200.19473953659536086No Hit
TAGCAGCACGTAAATATTGGC129510.18793381061449466No Hit
CGCGACCTCAGATCAGACGTGGCG126510.18358046776959092No Hit
GGAATACCGGGTGCTGTAGGCTT126450.18349340091269284No Hit
CCCCCCACTGCTAAATTTGACTGG123680.17947381435256504No Hit
CGCGACCTCAGATCAGACGTGG122520.17779052178586893No Hit
CCCCCCACTGCTAAATTTGACTGGCT116400.1689097023822653No Hit
TAGCTTATCAGACTGATGTTGACA114990.16686363124516054No Hit
TATTGCACTTGTCCCGGCCTGTT112590.16338095696923755No Hit
TTCAAGTAATCCAGGATAGGCTT109960.15956452640853858No Hit
TTCACAGTGGCTAAGTTCTGC107130.15545787299151273No Hit
TGAGAACTGAATTCCATGGGTT104310.15136573071730322No Hit
CACTAGATTGTGAGCTCCTGGA101810.14773794501321674No Hit
TGAGGTAGTAGGTTGTGTGGTT100440.14574991844737736No Hit
TTCAAGTAATCCAGGATAGGC99360.14418271502321203No Hit
ACGCCTGTCTGAGCGTCGCT97400.14133853103120825No Hit
AATGCACCCGGGCAAGGATTCT95860.13910381503749097No Hit
ACCCCCCACTGCTAAATTTGACTG95790.13900223703777656No Hit
TTATAAAGCAATGAGACTGATT93870.13621609761703818No Hit
CAAAGAATTCTCCTTTTGGGCT91000.1320513996287469No Hit
CAACGGAATCCCAAAAGCAGCTGA89970.1305567519186633No Hit
TGAGGTAGTAGGTTGTATAGT89170.12939586049335564No Hit
TTCACAGTGGCTAAGTTCCG88920.129033081922947No Hit
TATTGCACTTGTCCCGGCCTGTAG87870.12750941192723067No Hit
TCGTTTCCCGGCCAATGCACCA85140.12354786993836828No Hit
CTCCCACTGCTTCACTTGACTAGCCT83250.1208052639460789No Hit
CACTAGATTGTGAGCTCCTGGAA81570.11836739195293282No Hit
CAAAGTGCTGTTCGTGCAGGT79800.1157989196744396No Hit
TGTCAGTTTGTCAAATACCCCA78320.11365127053762042No Hit
TTCACAGTGGCTAAGTTCTG76010.11029919654704454No Hit
AGAGGTAGTAGGTTGCATAGTT74640.10831116998120517No Hit
TGTCAGTTTGTCAAATACCCCAA74430.1080064359820619No Hit
CCCTGTCTGAGCGTCGCT73140.10613449855875329No Hit
TGTAAACATCCCCGACTGGAAGC72940.10584427570242637No Hit
TGAGGTAGTAAGTTGTATTGTT71580.10387076027940335No Hit
CAAAGAATTCTCCTTTTGGGCTT69560.10093950943050149No Hit
CGCGACCTCAGATCAGACGTGGC69220.10044613057474575No Hit
TGAATACCGGGTGCTGTAGGCTTT69030.10017041886123516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)