FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9846929
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG103674410.528602369327533No Hit
GCCTGTCTGAGCGTCGCT5892445.984038272236958No Hit
CCCCCCACTGCTAAATTTGACTG4968615.045847289038035No Hit
TTCAAGTAATCCAGGATAGGCT2892722.937687475963318No Hit
TAGCAGCACGTAAATATTGGCG2589852.630109346782129No Hit
CCCCCACTGCTAAATTTGACTG2129862.162968779403203No Hit
AACATTCAACGCTGTCGGTGAGT1838811.8673943927086303No Hit
TAGCTTATCAGACTGATGTTGAC1568701.5930855193532927No Hit
AAGCTGCCAGTTGAAGAACTGT1562361.5866469637386436No Hit
AACATTCAACGCTGTCGGTGAG1473431.4963345424751209No Hit
TGAGGTAGTAGGTTGTATAGTT1256551.2760831321115447No Hit
TCGTACGACTCTTAGCGG1176781.195073103502625No Hit
TAGCTTATCAGACTGATGTTGACT1123121.1405789561395234No Hit
TAGCTTATCAGACTGATGTTGA962830.9777972401344622No Hit
TCCTGTCTGAGCGTCGCT843490.8566020939117159No Hit
TATTGCACTTGTCCCGGCCTGTAA774580.7866208845417694No Hit
CCCCCACTGCTAAATTTGACTGGCTT741050.752569659027703No Hit
TTCAAGTAATTCAGGATAGGTT633850.643703229707455No Hit
TATTGCACTTGTCCCGGCCTGT618450.6280638359431656No Hit
TGTAAACATCCTTGACTGGAAGCT575900.5848523940814441No Hit
ACTGGACTTGGAGTCAGAAGGC574400.5833290765070003No Hit
CACCCGGCTGTGTGCACATGTGC573330.5822424433038971No Hit
TATTGCACTTGTCCCGGCCTGTAT562750.5714979766788203No Hit
TGAGGTAGTAGATTGTATAGTT534980.543296290650618No Hit
CAACGGAATCCCAAAAGCAGCTGT526220.5344001160158665No Hit
AGCAGCATTGTACAGGGCTATGA512610.5205785478904134No Hit
CATAAAGTAGAAAGCACTACT509770.5176943999494665No Hit
AACCGGGCGGAAACACCA451760.45878263162047783No Hit
CCCATAAAGTAGAAAGCACTAC444640.4515519508671181No Hit
CAACGGAATCCCAAAAGCAGCT404010.4102903555006845No Hit
TATTGCACTTGTCCCGGCCTGTA394490.4006223666282147No Hit
CATTGCACTTGTCTCGGTCTGA359440.365027512638712No Hit
TGTAAACATCCCCGACTGGAAGCT354370.3598786992370921No Hit
CCCCCCACTGCTAAATTTGACTGGCT335970.3411926703239152No Hit
TTCTCACTACTGCACTTGACTAG301470.30615636611170854No Hit
CTCCCACTGCTTCACTTGACTAG286690.2911466102781893No Hit
TAGCAGCACGTAAATATTGGC286650.2911059884762041No Hit
CGCCTGTCTGAGCGTCGCT282620.28701334192619854No Hit
GGAATACCGGGTGCTGTAGGCTTT238770.24248169149995902No Hit
ACCGGGTGCTGTAGGCTT235740.2394045899995826No Hit
CCCATAAAGTAGAAAGCACT227220.23075214617674203No Hit
CTCCCACTGCTTCACTTGACTAGCCT221310.22475027493343355No Hit
CAACACCAGTCGATGGGCTGT218860.22226218956184207No Hit
CTTCTCACTACTGCACTTGACTAG216540.21990612504670237No Hit
TAGCTTATCAGACTGATGTTGACA215080.21842342927424377No Hit
AACGGAATCCCAAAAGCAGCTG215080.21842342927424377No Hit
CAAAGAATTCTCCTTTTGGGCT201880.20501823461913862No Hit
GGAATACCGGGTGCTGTAGGCTT195880.19892496432136353No Hit
CCCCCCACTGCTAAATTTGACTGG193200.19620330358835736No Hit
CTACGCCTGTCTGAGCGTCGCT183490.18634236115645803No Hit
ACCCCCCACTGCTAAATTTGACTG181700.18452453551762177No Hit
TGTAAACATCCTACACTCTCAGCT178770.1815489885222083No Hit
TCGTTTCCCGGCCAATGCACCA174600.17731416566525462No Hit
AGCTCGGTCTGAGGCCCCTCAGT171520.17418628691239674No Hit
CACTAGATTGTGAGCTCCTGGA159570.16205052356932806No Hit
AACATTCAACGCTGTCGGTGAGTT156810.1592476192323515No Hit
TGAGGTAGTAGTTTGTACAGTT153370.15575414426162715No Hit
TGAGGTAGTAGTTTGTGCTGTT150720.15306294988010982No Hit
TGATGTAGTAGGTTGTATAGTT142050.14425817429982485No Hit
TTCACAGTGGCTAAGTTCCGC141780.14398397713642497No Hit
TTCAAGTAATCCAGGATAGGCTT140010.14218646239858132No Hit
CACTAGATTGTGAGCTCCTGGAA136250.1383680130119756No Hit
CGCGACCTCAGATCAGACGTGGC134960.137057959897954No Hit
TTCAAGTAATCCAGGATAGGC128650.13064987063479386No Hit
ACCGGGTGCTGTAGGCTTT127230.12920779666432042No Hit
CCCCCACTGCTAAATTTGACTGGCT126650.1286187805355355No Hit
TTCACAGTGGCTAAGTTCTGC121590.12348012258441186No Hit
CAAAGAATTCTCCTTTTGGGCTT121220.12310437091604906No Hit
TGTCAGTTTGTCAAATACCCCA120540.1224138002823012No Hit
TGAGGTAGTAGGTTGTGTGGTT119490.12134747798019058No Hit
ACTGGACTTGGAGTCAGAAGGCT117310.11913358977199896No Hit
TACGCCTGTCTGAGCGTCGCT116310.1181180447223698No Hit
ACCGGGTGCTGTAGGCTTA113300.11506125412298596No Hit
CGCGACCTCAGATCAGACGTGGCG109730.11143575829580979No Hit
TGTCAGTTTGTCAAATACCCCAA106010.10765793071118923No Hit
TAGCAGCACGTAAATATTGGCGT105020.10665254111205637No Hit
TATTGCACTTGTCCCGGCCTGTT101350.10292549077991726No Hit
GGGAATACCGGGTGCTGTAGGCTT99580.10112797604207363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)