FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8215550
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110408413.438954178356896No Hit
CAACGGAATCCCAAAAGCAGCTG8188149.966636439434975No Hit
TAGCAGCACGTAAATATTGGCG2830433.445210606715314No Hit
AACATTCAACGCTGTCGGTGAGT2825923.439721016852189No Hit
TTCAAGTAATCCAGGATAGGCT2756103.3547358363104114No Hit
CCCCCACTGCTAAATTTGACTG2484553.024204100760144No Hit
AACATTCAACGCTGTCGGTGAG1945402.3679485853046964No Hit
AAGCTGCCAGTTGAAGAACTGT1815602.209955511195233No Hit
TATTGCACTTGTCCCGGCCTGTAA1559291.8979739640072788No Hit
TATTGCACTTGTCCCGGCCTGTAT1307701.5917376195142139No Hit
TAGCTTATCAGACTGATGTTGACT1289211.5692315182793606No Hit
TAGCTTATCAGACTGATGTTGAC1125541.3700117460182215No Hit
TAGCTTATCAGACTGATGTTGA823921.0028786873672486No Hit
TGAGGTAGTAGGTTGTATAGTT766590.9330963842956345No Hit
TGTAAACATCCTTGACTGGAAGCT759440.9243933759760453No Hit
TTCAAGTAATTCAGGATAGGTT647810.7885168978339855No Hit
GGAATACCGGGTGCTGTAGGCTTT607560.7395244384125226No Hit
TATTGCACTTGTCCCGGCCTGT565110.68785413027734No Hit
TGAGGTAGTAGATTGTATAGTT565040.6877689260000852No Hit
TGTAAACATCCCCGACTGGAAGCT526410.6407483369950886No Hit
CAACGGAATCCCAAAAGCAGCTGT491770.5985843917936109No Hit
TATTGCACTTGTCCCGGCCTGTA467260.5687507227148517No Hit
CATAAAGTAGAAAGCACTACT415270.5054682887938118No Hit
CTTCTCACTACTGCACTTGACTAG407720.49627839888991No Hit
AGCAGCATTGTACAGGGCTATGA397980.48442283231189637No Hit
GGAATACCGGGTGCTGTAGGCTT393320.47875066185465365No Hit
TTCTCACTACTGCACTTGACTAG379280.4616611182452788No Hit
ACTGGACTTGGAGTCAGAAGGC321080.3908198477277845No Hit
CACCCGGCTGTGTGCACATGTGC319210.38854367632112274No Hit
CAAAGAATTCTCCTTTTGGGCT294600.3585882868462854No Hit
ACCCCCCACTGCTAAATTTGACTG289520.35240489072551445No Hit
CCCCCACTGCTAAATTTGACTGGCTT266390.32425096311263396No Hit
CTCCCACTGCTTCACTTGACTAG249120.30322985070993425No Hit
TGAATACCGGGTGCTGTAGGCTTT247080.30074675462993955No Hit
CAACGGAATCCCAAAAGCAGCT246130.29959041086719695No Hit
CCCCCCACTGCTAAATTTGACTGG245770.2991522174413156No Hit
CCCATAAAGTAGAAAGCACTAC243850.2968151858366147No Hit
CCCCCCACTGCTAAATTTGACTGGCT233590.2843266731989946No Hit
TGAGGTAGTAGTTTGTACAGTT212350.25847326107199153No Hit
CATTGCACTTGTCTCGGTCTGA210900.25670831532885807No Hit
TGATGTAGTAGGTTGTATAGTT210700.25646487453670175No Hit
CTCCCACTGCTTCACTTGACTAGCCT206930.25187601560455475No Hit
TAGCAGCACGTAAATATTGGC204150.24849218859358166No Hit
TAGCTTATCAGACTGATGTTGACA202930.24700719976142801No Hit
CAAAGAATTCTCCTTTTGGGCTT190440.23180432229126471No Hit
AACATTCAACGCTGTCGGTGAGTT187910.22872479627048709No Hit
TGTAAACATCCTACACTCTCAGCT174060.2118665214136607No Hit
TGTCAGTTTGTCAAATACCCCAA172680.21018677994778193No Hit
CACTAGATTGTGAGCTCCTGGAA171690.20898174802660807No Hit
TATTGCACTTGTCCCGGCCTGTAG170270.2072533184022981No Hit
TATTGCACTTGTCCCGGCCTGTAAT163070.19848944988466993No Hit
TGAATACCGGGTGCTGTAGGCTT159910.19464308536859978No Hit
TGATGTAGTAGATTGTATAGTT156910.19099147348625473No Hit
CACTAGATTGTGAGCTCCTGGA153730.18712076489096896No Hit
TGTAAACATCCCCGACTGGAAGC152690.185854872771756No Hit
CCCATAAAGTAGAAAGCACT144190.17550863910511166No Hit
TATTGCACTTGTCCCGGCCTGTAAA142500.1734515644113906No Hit
AACGGAATCCCAAAAGCAGCTG141170.17183268314355093No Hit
TTCAAGTAATCCAGGATAGGCTT138870.16903311403375307No Hit
CCCCCCACTGCTAAATTTGACTGT127180.15480399973221512No Hit
TCGTTTCCCGGCCAATGCACCA121620.14803634571026894No Hit
TGTCAGTTTGTCAAATACCCCA120570.14675828155144818No Hit
TATTGCACTTGTCCCGGCCTGTT120080.14616185161066514No Hit
TGAGGTAGTAGTTTGTGCTGTT119890.14593058285811664No Hit
AGCTCGGTCTGAGGCCCCTCAGT111580.13581561794402078No Hit
CCCCCCACTGCTAAATTTGACTGTA110870.1349514031318658No Hit
ATGCACCTGGGCAAGGATTCTGA110850.13492705905265015No Hit
GACCCGCGGGCGCTCTCCAGTCCT109610.13341772614128086No Hit
ACTGGACTTGGAGTCAGAAGGCT108270.1317866728338334No Hit
TATTGCACTTGTCCCGGCCTGTTT108130.13161626427932396No Hit
CAACGGAATCCCAAAAGCAGCTGA107250.1305451247938361No Hit
TTCAAGTAATCCAGGATAGGC106070.12910882412011368No Hit
CCCGCCACTGCTAAATTTGACTG104240.12688134087188319No Hit
TGAGAACTGAATTCCATAGGCTGT101550.12360706221738046No Hit
ACTGGACTTGGAGTCAGAAGGCA100140.12189080463267828No Hit
ACTTGACTTGGAGTCAGAAGGC98160.11948074079033053No Hit
TTCACAGTGGCTAAGTTCCGC96120.1169976447103359No Hit
TTCACAGTGGCTAAGTTCTGC94500.11502577429386956No Hit
GGAATACCGGGTGCTGTAGGCTTA93560.11388160257073476No Hit
CAACACCAGTCGATGGGCTGT89410.10883020613349076No Hit
TCGTACGACTCTTAGCGG89190.10856242126211879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)