FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9529206
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG96627510.140141791456706No Hit
CAACGGAATCCCAAAAGCAGCTG7516417.8877610579517325No Hit
AACATTCAACGCTGTCGGTGAGT4129654.33367690865325No Hit
TTCAAGTAATCCAGGATAGGCT3237683.397638795929063No Hit
CCCCCACTGCTAAATTTGACTG2756742.8929377746687397No Hit
TAGCAGCACGTAAATATTGGCG2671712.8037068355957464No Hit
AACATTCAACGCTGTCGGTGAG2425602.5454376786481476No Hit
AAGCTGCCAGTTGAAGAACTGT2203242.3120918993670614No Hit
TATTGCACTTGTCCCGGCCTGTAA1760051.8470059310293008No Hit
TGAGGTAGTAGGTTGTATAGTT1591071.6696774106887815No Hit
TATTGCACTTGTCCCGGCCTGTAT1513031.5877818151900587No Hit
GGAATACCGGGTGCTGTAGGCTTT1314251.3791810146616623No Hit
TATTGCACTTGTCCCGGCCTGT1204501.2640087747079871No Hit
TAGCTTATCAGACTGATGTTGACT1182771.2412051958998473No Hit
TAGCTTATCAGACTGATGTTGAC1145481.2020728694499836No Hit
TGAGGTAGTAGATTGTATAGTT1109011.163801055407974No Hit
TAGCTTATCAGACTGATGTTGA1103301.1578089507142568No Hit
GGAATACCGGGTGCTGTAGGCTT1010161.0600673340465092No Hit
GCCTGTCTGAGCGTCGCT980721.0291728397937878No Hit
TTCAAGTAATTCAGGATAGGTT832780.8739238085523601No Hit
TATTGCACTTGTCCCGGCCTGTA738260.7747340124665161No Hit
TGTAAACATCCTTGACTGGAAGCT710540.7456444954595378No Hit
CATAAAGTAGAAAGCACTACT636400.6678415809250005No Hit
ACTGGACTTGGAGTCAGAAGGC611810.6420367027431246No Hit
AGCAGCATTGTACAGGGCTATGA593280.6225912211363674No Hit
TGTAAACATCCCCGACTGGAAGCT537630.5641918119935702No Hit
TTAATGCTAATCGTGATAGGGGTT499090.5237477288244162No Hit
TGAGGTAGTAGTTTGTACAGTT423860.44480096243065786No Hit
CATTGCACTTGTCTCGGTCTGA402020.42188194903122045No Hit
TTCTCACTACTGCACTTGACTAG385710.40476614735792255No Hit
CAACGGAATCCCAAAAGCAGCTGT382300.4011876750276991No Hit
CACCCGGCTGTGTGCACATGTGC336420.3530409564028734No Hit
TGAGAACTGAATTCCATGGGTT334120.3506273240393796No Hit
CTTCTCACTACTGCACTTGACTAG326350.342473444272272No Hit
CGCCTGTCTGAGCGTCGCT321620.3375097568464781No Hit
TGAGGTAGTAGTTTGTGCTGTT288630.30288987351097246No Hit
CTCCCACTGCTTCACTTGACTAG277060.29074825331722287No Hit
AACATTCAACGCTGTCGGTGAGTT263750.27678066777022137No Hit
CCCATAAAGTAGAAAGCACT249880.2622254152129779No Hit
TGTCAGTTTGTCAAATACCCCAA229490.24082803960791696No Hit
AACGGAATCCCAAAAGCAGCTG227400.23863478237326385No Hit
CCCATAAAGTAGAAAGCACTAC221810.23276860632459828No Hit
TCGTACGACTCTTAGCGG215530.2261783405668846No Hit
CAACACCAGTCGATGGGCTGT214360.224950536277629No Hit
CCCCCCACTGCTAAATTTGACTGG210860.22127761746361657No Hit
CAACGGAATCCCAAAAGCAGCT210560.22096279585098696No Hit
ATCCCGGACGAGCCCCCA205770.21593614410266712No Hit
GGAATACCGGGTGCTGTAGGCTTA200740.21065763506424354No Hit
TATTGCACTTGTCCCGGCCTGTT197190.20693224598145954No Hit
TATTGCACTTGTCCCGGCCTGTAG193150.20269264826471375No Hit
TGAGAACTGAATTCCATAGGCTGT191920.20140187965293227No Hit
CACTAGATTGTGAGCTCCTGGA190460.19986974780480138No Hit
TGTAAACATCCTACACTCTCAGCT171690.18017240890794048No Hit
TAGCAGCACGTAAATATTGGC170070.17847237219974046No Hit
TGAGGTAGTAGGTTGTGTGGTT169480.1778532230282355No Hit
ACTGGACTTGGAGTCAGAAGGCA168820.1771606154804503No Hit
TTCAAGTAATCCAGGATAGGCTT167230.17549206093351324No Hit
ACCCCCCACTGCTAAATTTGACTG165230.173393250182649No Hit
TGAGGTAGTAGGTTGTATAGT164730.17286854749493294No Hit
CCCCCACTGCTAAATTTGACTGGCTT162630.1706647962065255No Hit
AACCGGGCGGAAACACCA161560.16954193245481314No Hit
CAAAGAATTCTCCTTTTGGGCT159370.1672437346826168No Hit
ACTGGACTTGGAGTCAGAAGGCT159190.167054841715039No Hit
TTATAAAGCAATGAGACTGATT157350.1651239358242439No Hit
TGTAAACATCCCCGACTGGAAGC155960.16366526235239326No Hit
TAGCTTATCAGACTGATGTTGACA154870.16252141049317226No Hit
CACTAGATTGTGAGCTCCTGGAA152320.15984542678582037No Hit
GGAATACCGGGTGCTGTAGGCT150790.1582398365614092No Hit
GACCCGCGGGCGCTCTCCAGTCCT147420.15470334044620296No Hit
CCCCCCACTGCTAAATTTGACTGGCT146480.15371689939329677No Hit
ACTGGACTTGGAGTCAGAAGG143490.15057917732075474No Hit
CAAAGAATTCTCCTTTTGGGCTT141990.14900506925760656No Hit
TTCAAGTAATCCAGGATAGGC138450.14529017422857685No Hit
TATTGCACTTGTCCCGGCCTGTAAT137770.14457657857328302No Hit
TGTCAGTTTGTCAAATACCCCA137710.1445136142507571No Hit
ACCGGGTGCTGTAGGCTT131880.13839558091198784No Hit
CCCCCCACTGCTAAATTTGACTGT131650.13815421767563846No Hit
TTCACAGTGGCTAAGTTCTG128100.13442882859285443No Hit
AACATTCAACGCTGTCGGTGA127500.13379918536759516No Hit
CTACGCCTGTCTGAGCGTCGCT127120.13340041132493097No Hit
CCCCCCACTGCTAAATTTGACTGTA125650.13185778542304574No Hit
AGCTCGGTCTGAGGCCCCTCAGT122320.1283632655228568No Hit
TGAGGTAGTAGATTGTATAGT121700.12771263419008885No Hit
TCGTTTCCCGGCCAATGCACCA120880.12685212178223454No Hit
CTCCCACTGCTTCACTTGACTAGCCT119120.12500516832147401No Hit
TATTGCACTTGTCCCGGCCTGTTT115580.1212902732924443No Hit
TACGCCTGTCTGAGCGTCGCT115530.12123780302367271No Hit
TTCACAGTGGCTAAGTTCCG113660.11927541497161462No Hit
TGAGGTAGTAAGTTGTATTGTT113050.11863527769260104No Hit
AGCAGCATTGTACAGGGCTATGT110940.11642103235043927No Hit
TTCACAGTGGCTAAGTTCCGC110140.11558150805009357No Hit
TTCACAGTGGCTAAGTTCTGC109510.11492038266357134No Hit
TATTGCACTTGTCCCGGCCTGTAAA109330.11473148969599356No Hit
GAATACCGGGTGCTGTAGGCTTT107080.11237032760127129No Hit
AACATTCAACGCTGTCGGTGAGA106070.11131042817208485No Hit
AGCAGCATTGTACAGGGCTATG106020.11125795790331325No Hit
TATTGCACTTGTCCCGGCCTGA105160.11035546928044161No Hit
CAAAGTGCTGTTCGTGCAGGT103090.10818320015329715No Hit
AATGACACGATCACTCCCGTTG100840.10582203805857487No Hit
ATCCCACCGCTGCCACCA98670.10354482839388718No Hit
ACCGGGTGCTGTAGGCTTT96690.10146700575053157No Hit
AATGCACCCGGGCAAGGATTCT96340.10109971386913033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)