FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7859998
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG108754513.836453902405573No Hit
CAACGGAATCCCAAAAGCAGCTG6988958.891796155673322No Hit
CCCCCACTGCTAAATTTGACTG3192584.0618076493149236No Hit
AACATTCAACGCTGTCGGTGAGT3143343.9991613229418124No Hit
AACATTCAACGCTGTCGGTGAG2180962.774758975765643No Hit
TTCAAGTAATCCAGGATAGGCT1688462.148168485539055No Hit
TATTGCACTTGTCCCGGCCTGTAA1679362.1365908744506044No Hit
TAGCAGCACGTAAATATTGGCG1513131.9251022710183898No Hit
TATTGCACTTGTCCCGGCCTGTAT1315811.6740589501422265No Hit
AAGCTGCCAGTTGAAGAACTGT1290701.6421123771278314No Hit
TAGCTTATCAGACTGATGTTGACT1256741.5989062592636791No Hit
TAGCTTATCAGACTGATGTTGAC1140121.4505347202378422No Hit
TAGCTTATCAGACTGATGTTGA809281.0296185826001483No Hit
GGAATACCGGGTGCTGTAGGCTTT802671.0212089112490867No Hit
TGAGGTAGTAGGTTGTATAGTT782070.9950002531807259No Hit
GCCTGTCTGAGCGTCGCT621950.7912851886221854No Hit
TATTGCACTTGTCCCGGCCTGT594580.7564632968099991No Hit
TATTGCACTTGTCCCGGCCTGTA554530.7055090853712686No Hit
GGAATACCGGGTGCTGTAGGCTT520190.661819506824302No Hit
TGAATACCGGGTGCTGTAGGCTTT519850.6613869367396785No Hit
TGAGGTAGTAGATTGTATAGTT502830.6397329872094115No Hit
TTAATGCTAATCGTGATAGGGGTT485410.6175701316972345No Hit
TGTAAACATCCTTGACTGGAAGCT457140.581603201425751No Hit
AGCAGCATTGTACAGGGCTATGA450100.5726464561441365No Hit
TCCTGTCTGAGCGTCGCT434690.5530408531910568No Hit
TGTAAACATCCCCGACTGGAAGCT425200.540967058770244No Hit
TTCAAGTAATTCAGGATAGGTT388400.49414770843453143No Hit
CAACGGAATCCCAAAAGCAGCTGT381280.48508918195653483No Hit
ACTGGACTTGGAGTCAGAAGGC355560.4523665273197271No Hit
TGAATACCGGGTGCTGTAGGCTT336930.42866423121227254No Hit
CATAAAGTAGAAAGCACTACT326300.4151400547430165No Hit
CACCCGGCTGTGTGCACATGTGC319310.40624692270914065No Hit
CAACGGAATCCCAAAAGCAGCT310730.3953308893971729No Hit
CATTGCACTTGTCTCGGTCTGA283270.3603944937390569No Hit
TGATGTAGTAGGTTGTATAGTT280240.35653953092608925No Hit
AACATTCAACGCTGTCGGTGAGTT204210.25980922641456144No Hit
CACTAGATTGTGAGCTCCTGGAA196180.24959293882771982No Hit
TAGCTTATCAGACTGATGTTGACA194750.24777359994239187No Hit
CACTAGATTGTGAGCTCCTGGA193530.24622143669756658No Hit
TATTGCACTTGTCCCGGCCTGTAG193050.2456107495192747No Hit
CCCATAAAGTAGAAAGCACTAC192490.24489828114460083No Hit
CAAAGAATTCTCCTTTTGGGCT191020.24302805166108182No Hit
TGAGGTAGTAGTTTGTACAGTT182520.2322137995454961No Hit
TGATGTAGTAGATTGTATAGTT181360.2307379721979573No Hit
TCGTACGACTCTTAGCGG180550.2297074375845897No Hit
ACCCCCCACTGCTAAATTTGACTG176760.22488555340599323No Hit
CCCCCACTGCTAAATTTGACTGGCTT170710.21718835042960571No Hit
CCCATAAAGTAGAAAGCACT170470.21688300684045975No Hit
CTCCCCACTGCTAAATTTGACTG168820.21478376966508136No Hit
CTTCTCACTACTGCACTTGACTAG168340.21417308248678946No Hit
TTCTCACTACTGCACTTGACTAG162070.20619598122035143No Hit
TATTGCACTTGTCCCGGCCTGTAAT161250.2051527239574361No Hit
CGCCCCACTGCTAAATTTGACTG155140.19737918508376212No Hit
CAACACCAGTCGATGGGCTGT154850.19701022824687742No Hit
TGAGAACTGAATTCCATAGGCTGT154500.1965649355127062No Hit
TATTGCACTTGTCCCGGCCTGTAAA150330.19125959065129533No Hit
TCGTTTCCCGGCCAATGCACCA149620.19035628253340522No Hit
ACTTGACTTGGAGTCAGAAGGC140850.17919851888003024No Hit
CCCGCCACTGCTAAATTTGACTG138000.17557256375892208No Hit
CAAAGAATTCTCCTTTTGGGCTT137830.17535627871661036No Hit
CCCCCCACTGCTAAATTTGACTGG129350.16456747190012008No Hit
TGAGAACTGAATTCCATGGGTT126220.16058528259167495No Hit
ACTGGACTTGGAGTCAGAAGGCA124210.1580280300325776No Hit
TGTCAGTTTGTCAAATACCCCAA121830.1550000394402136No Hit
AACGGAATCCCAAAAGCAGCTG121390.15444024286011268No Hit
TAGCAGCACGTAAATATTGGC121230.15423668046734873No Hit
TGTAAACATCCTACACTCTCAGCT118260.15045805355166755No Hit
GGAATACCGGGTGCTGTAGGCTTA118010.15013998731297387No Hit
TGAGGTAGTAGTTTGTGCTGTT116250.14790080099257025No Hit
TATTGCACTTGTCCCGGCCTGTT114170.14525448988663867No Hit
CCCCCCACTGCTAAATTTGACTGT112030.14253184288342058No Hit
CCCCCCACTGCTAAATTTGACTGTA111700.1421119954483449No Hit
CTCCCACTGCTTCACTTGACTAG108890.1385369309254277No Hit
TTCAAGTAATCCAGGATAGGCTT105960.13480919460793758No Hit
ACTGGACTTGGAGTCAGAAGGCT105880.13470741341155557No Hit
CCCCCCACTGCTAAATTTGACTGGCT102550.13047077111215552No Hit
TTATAAAGCAATGAGACTGATT100980.12847331513315907No Hit
CAACGGAATCCCAAAAGCAGCTGA99700.12684481599104733No Hit
TGTAAACATCCCCGACTGGAAGC94790.12059799506310308No Hit
AGCAGCATTGTACAGGGCTATGT92550.11774812156440753No Hit
ATGCACCTGGGCAAGGATTCTGA92330.11746822327435708No Hit
AACCGGGCGGAAACACCA90280.11486008011706873No Hit
TGCAAGTAATCCAGGATAGGCT86710.1103180942285227No Hit
TATTGCACTTGTCCCGGCCTGTTT86050.10947839935837134No Hit
TTAATGCTAATCGTGATAGGGGTTT85050.10820613440359654No Hit
AGCTACATCTGGCTACTGGGTCTC84210.10713743184158572No Hit
CGCCTGTCTGAGCGTCGCT83910.10675575235515326No Hit
TGTCAGTTTGTCAAATACCCCA83730.1065267446632938No Hit
CTCCCACTGCTTCACTTGACTAGCCT82010.10433844894108116No Hit
CACCCGTAGAACCGACCTTGCG81830.10410944124922168No Hit
GCCCCCACTGCTAAATTTGACTG80930.10296440278992439No Hit
GGAATACCGGGTGCTGTAGGCT79980.10175575108288831No Hit
AACATTCAACGCTGTCGGTGAGA78880.10035625963263604No Hit
TGAATACCGGGTGCTGTAGGCTTA78760.10020358783806307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)