FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10077647
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8587908.52173131287492No Hit
CAACGGAATCCCAAAAGCAGCTG5654195.610625178675141No Hit
AACATTCAACGCTGTCGGTGAGT4209044.176609877285839No Hit
TAGCAGCACGTAAATATTGGCG3216123.191340200743289No Hit
TTCAAGTAATCCAGGATAGGCT3032833.009462427092356No Hit
AAGCTGCCAGTTGAAGAACTGT2635972.6156601833741546No Hit
CCCCCACTGCTAAATTTGACTG2274802.257272952704138No Hit
GCCTGTCTGAGCGTCGCT2161312.14465737885044No Hit
AACATTCAACGCTGTCGGTGAG1944801.9298155611126289No Hit
TAGCTTATCAGACTGATGTTGAC1400421.3896299404017625No Hit
TAGCTTATCAGACTGATGTTGACT1169481.160469303995268No Hit
TATTGCACTTGTCCCGGCCTGTAA1109071.100524755431501No Hit
TAGCTTATCAGACTGATGTTGA1044571.0365217198022514No Hit
TGAGGTAGTAGGTTGTATAGTT924570.9174463046780662No Hit
TATTGCACTTGTCCCGGCCTGTAT884110.877298043878695No Hit
CATAAAGTAGAAAGCACTACT849730.843182937445616No Hit
TATTGCACTTGTCCCGGCCTGT829930.8235354939501255No Hit
GGAATACCGGGTGCTGTAGGCTTT805380.7991746486059692No Hit
CGCCTGTCTGAGCGTCGCT801550.7953741582732556No Hit
TGTAAACATCCTTGACTGGAAGCT752390.7465929298773811No Hit
TCCTGTCTGAGCGTCGCT736170.7304979029330954No Hit
AGCAGCATTGTACAGGGCTATGA681620.6763682038078929No Hit
GGAATACCGGGTGCTGTAGGCTT643890.6389289087025969No Hit
TATTGCACTTGTCCCGGCCTGTA551780.5475286046435244No Hit
ACTGGACTTGGAGTCAGAAGGC507750.5038378502442088No Hit
TGAGGTAGTAGATTGTATAGTT507220.5033119338274104No Hit
TTCAAGTAATTCAGGATAGGTT477410.4737316161203107No Hit
TGTAAACATCCCCGACTGGAAGCT448370.4449153656602578No Hit
AACATTCAACGCTGTCGGTGAGTT384910.38194431696208453No Hit
TTCTCACTACTGCACTTGACTAG367860.36502568506318983No Hit
CAACGGAATCCCAAAAGCAGCTGT362280.35948867825991526No Hit
CCCATAAAGTAGAAAGCACTAC354450.35171900742306217No Hit
CTACGCCTGTCTGAGCGTCGCT344050.34139913811229944No Hit
TACGCCTGTCTGAGCGTCGCT325600.32309129303695594No Hit
CTTCTCACTACTGCACTTGACTAG320290.3178222059177107No Hit
TGTAAACATCCTACACTCTCAGCT316600.31416063690264207No Hit
CTCCCACTGCTTCACTTGACTAG306130.30377130693305693No Hit
TGAATACCGGGTGCTGTAGGCTTT298010.2957138705096537No Hit
CCCCCACTGCTAAATTTGACTGGCTT297780.2954856426306657No Hit
CATTGCACTTGTCTCGGTCTGA283590.28140497479223076No Hit
TCGTACGACTCTTAGCGG275630.27350630558899314No Hit
CAACGGAATCCCAAAAGCAGCT267000.26494279865131215No Hit
ACGCCTGTCTGAGCGTCGCT256990.25500992443970305No Hit
TTCACAGTGGCTAAGTTCCG245410.24351914688021917No Hit
TGAATACCGGGTGCTGTAGGCTT237710.2358784744097506No Hit
TGAGGTAGTAGTTTGTACAGTT234210.23240544146862852No Hit
CCCCCCACTGCTAAATTTGACTGGCT232630.23083761516949344No Hit
CCCCCCACTGCTAAATTTGACTGG231420.22963693806699123No Hit
TAGCAGCACATCATGGTTTACA225730.22399077879985277No Hit
GTCAGGATGGCCGAGCGGTCTAAGG225330.22359386074943885No Hit
GCGTTCGCGCTTTCCCCTG208910.20730037477994617No Hit
CCCATAAAGTAGAAAGCACT197670.19614697756331415No Hit
TGTCAGTTTGTCAAATACCCCAA190170.18870476411805256No Hit
TCGTTTCCCGGCCAATGCACCA182810.18140147199043585No Hit
TAGCTTATCAGACTGATGTTGACA181780.18037940801061994No Hit
TGATGTAGTAGGTTGTATAGTT181160.17976418503247832No Hit
TTCACAGTGGCTAAGTTCCGC177930.17655907177538566No Hit
CACCCGGCTGTGTGCACATGTGC177150.17578508157707845No Hit
AACCGGGCGGAAACACCA176400.1750408602325523No Hit
CAAAGAATTCTCCTTTTGGGCT175090.1737409536174466No Hit
TAGCAGCACGTAAATATTGGC164730.1634607761117253No Hit
TGAGGTAGTAGTTTGTGCTGTT164720.16345085316046495No Hit
ACTGGACTTGGAGTCAGAAGGCA152360.15118608540267386No Hit
TATTGCACTTGTCCCGGCCTGTT148170.14702836882458772No Hit
CAACACCAGTCGATGGGCTGT147960.1468199868481204No Hit
TGTAAACATCCTACACTCAGCT146420.14529185235402667No Hit
TTCAAGTAATCCAGGATAGGCTT145810.1446865523271454No Hit
TTCAAGTAATCCAGGATAGGC139940.13886177993732068No Hit
CCAAGTCCTTCTGATCGAGGCCC139830.13875262747345685No Hit
AGCAGCATTGTACAGGGCTATGT136560.1355078224113228No Hit
TGAGGTAGTAGGTTGTGTGGTT134250.13321562067018225No Hit
TCAGGCTCAGTCCCCTCCCGAT134140.1331064682063184No Hit
TAGCAGCACGTAAATATTGGCGT133620.13259047474078026No Hit
TTATAAAGCAATGAGACTGATT133600.13257062883825957No Hit
ACTGGACTTGGAGTCAGAAGG133510.13248132227691642No Hit
AGCTCGGTCTGAGGCCCCTCAGT133360.13233247800801118No Hit
ATCACATTGCCAGGGATTTCC133260.1322332484954077No Hit
TGTAAACATCCCCGACTGGAAGC130210.12920674836100135No Hit
CAAAGAATTCTCCTTTTGGGCTT128210.1272221581089316No Hit
TGTCAGTTTGTCAAATACCCCA126570.1255947941022344No Hit
CAAAGTGCTGTTCGTGCAGGT126110.12513833834425836No Hit
ACCCCCCACTGCTAAATTTGACTG123990.12303467267706439No Hit
TTCACAGTGGCTAAGTTCTGC123310.12235991199136068No Hit
AACGGAATCCCAAAAGCAGCTG122760.1218141496720415No Hit
CTCCCACTGCTTCACTTGACTAGCCT122180.12123861849894127No Hit
TATTGCACTTGTCCCGGCCTGTAG119750.11882734134267652No Hit
AACATTCAACGCTGTCGGTGAGA119200.11828157902335733No Hit
ACTTGACTTGGAGTCAGAAGGC118870.11795412163176583No Hit
CAAAGTGCTGTTCGTGCAGGTAG118130.11721982323850003No Hit
AGCAGCATTGTACAGGGCTATG118070.11716028553093792No Hit
ATCACATTGCCAGGGATTTCCA117320.11641606418641176No Hit
GGAATACCGGGTGCTGTAGGCTTA115210.11432232147047817No Hit
GACCCGCGGGCGCTCTCCAGTCCT112020.11115690001842693No Hit
ACCACAGGGTAGAACCACGGAC111260.11040275572264041No Hit
CCTCGACACAAGGGTTTGT106670.10584812109414032No Hit
TTCACAGTGGCTAAGTTCTG104730.10392306854963268No Hit
ACTGGACTTGGAGTCAGAAGGCT103110.10231555044545616No Hit
AACATTCAACGCTGTCGGTGA102170.10138279302698337No Hit
TGATGTAGTAGATTGTATAGTT102130.10134310122194198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)