FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8450009
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG112533913.317607117341531No Hit
CAACGGAATCCCAAAAGCAGCTG6742987.979849488917703No Hit
AACATTCAACGCTGTCGGTGAGT3347763.961841934132851No Hit
TAGCAGCACGTAAATATTGGCG3074513.6384694974881095No Hit
AAGCTGCCAGTTGAAGAACTGT3041053.598871906526963No Hit
CCCCCACTGCTAAATTTGACTG2700483.1958309156830484No Hit
TTCAAGTAATCCAGGATAGGCT2625863.107523317430786No Hit
AACATTCAACGCTGTCGGTGAG1862502.2041396642299436No Hit
TATTGCACTTGTCCCGGCCTGTAT1317451.5591107654441552No Hit
TATTGCACTTGTCCCGGCCTGTAA1270111.503087156475218No Hit
TATTGCACTTGTCCCGGCCTGT1054471.247892162008348No Hit
TGAGGTAGTAGGTTGTATAGTT858001.015383533911029No Hit
TAGCTTATCAGACTGATGTTGACT854691.0114663783198337No Hit
GGAATACCGGGTGCTGTAGGCTTT767250.9079871985935163No Hit
TAGCTTATCAGACTGATGTTGAC756650.8954428332561539No Hit
TAGCTTATCAGACTGATGTTGA739230.8748274705979603No Hit
TGTAAACATCCTTGACTGGAAGCT613440.7259637238256196No Hit
TATTGCACTTGTCCCGGCCTGTA571040.6757862624761701No Hit
TGAGGTAGTAGATTGTATAGTT561140.6640702986233505No Hit
TTCAAGTAATTCAGGATAGGTT556590.6586856889738224No Hit
GGAATACCGGGTGCTGTAGGCTT552720.6541058121949929No Hit
TGTAAACATCCCCGACTGGAAGCT548380.6489697229908276No Hit
AGCAGCATTGTACAGGGCTATGA502860.5950999578817017No Hit
ACTGGACTTGGAGTCAGAAGGC443520.5248751806063165No Hit
CAACGGAATCCCAAAAGCAGCTGT418920.49576278557809816No Hit
CTTCTCACTACTGCACTTGACTAG398740.47188115420942156No Hit
CATAAAGTAGAAAGCACTACT386350.4572184479330141No Hit
CCCCCCACTGCTAAATTTGACTGG376380.44541964393174016No Hit
TTCTCACTACTGCACTTGACTAG343550.406567614306683No Hit
CTCCCACTGCTTCACTTGACTAG338380.4004492776279883No Hit
TGAATACCGGGTGCTGTAGGCTTT318910.3774078820507765No Hit
CATTGCACTTGTCTCGGTCTGA292360.3459877971727604No Hit
CGCCTGTCTGAGCGTCGCT276790.3275617812951442No Hit
TGAGGTAGTAGTTTGTACAGTT262060.31012984719897935No Hit
CACCCGGCTGTGTGCACATGTGC261310.3092422741798263No Hit
TGATGTAGTAGGTTGTATAGTT238090.2817630135068495No Hit
ACCCCCCACTGCTAAATTTGACTG231950.274496749056717No Hit
TGAATACCGGGTGCTGTAGGCTT222400.2631949859461688No Hit
CCCATAAAGTAGAAAGCACT220750.26124232530403224No Hit
AACATTCAACGCTGTCGGTGAGTT199620.2362364347777618No Hit
CCCCCCACTGCTAAATTTGACTGGCT196020.2319760842858274No Hit
CCCATAAAGTAGAAAGCACTAC180000.21301752459671935No Hit
CAACGGAATCCCAAAAGCAGCT177510.21007078217313144No Hit
TAGCAGCACGTAAATATTGGC176670.20907670039168003No Hit
TGTAAACATCCTACACTCTCAGCT176520.20889918578784944No Hit
TATTGCACTTGTCCCGGCCTGTT176280.20861516242172048No Hit
TGTAAACATCCCCGACTGGAAGC172040.20359741628677555No Hit
GCCTGTCTGAGCGTCGCT166170.1966506781235381No Hit
TGAGGTAGTAGTTTGTGCTGTT161790.1914672516916846No Hit
AGCTCGGTCTGAGGCCCCTCAGT161020.19055601005868752No Hit
TTCAAGTAATCCAGGATAGGCTT158160.18717139827898407No Hit
CAACACCAGTCGATGGGCTGT156760.18551459530989847No Hit
TATTGCACTTGTCCCGGCCTGTAG156730.18547909238913238No Hit
TGATGTAGTAGATTGTATAGTT155010.18344359159854148No Hit
CAAAGAATTCTCCTTTTGGGCT149340.17673353957374482No Hit
CCCCCACTGCTAAATTTGACTGGCTT146430.17328975625943122No Hit
AACGGAATCCCAAAAGCAGCTG144230.1706862087365824No Hit
AACCGGGCGGAAACACCA143350.1696447897274429No Hit
ACTGGACTTGGAGTCAGAAGGCT133400.15786965434001313No Hit
CTCCCACTGCTTCACTTGACTAGCCT132210.15646137181629038No Hit
CACTAGATTGTGAGCTCCTGGA129140.1528282395912241No Hit
CAAAGAATTCTCCTTTTGGGCTT127870.15132528261212502No Hit
ATCCCGGACGAGCCCCCA122410.1448637510326912No Hit
GGAATACCGGGTGCTGTAGGCTTA122130.1445323904388741No Hit
ACTTGACTTGGAGTCAGAAGGC121930.1442957043004333No Hit
TTCAAGTAATCCAGGATAGGC119970.14197618014371347No Hit
TATTGCACTTGTCCCGGCCTGTTT119780.1417513283121947No Hit
TGAGGTAGTAGGTTGTGTGGTT112740.13341997623907856No Hit
TGTCAGTTTGTCAAATACCCCAA111410.13184601341844723No Hit
GACCCGCGGGCGCTCTCCAGTCCT109910.13007086738014126No Hit
CTACGCCTGTCTGAGCGTCGCT108080.12790518921340793No Hit
TGAGAACTGAATTCCATAGGCTGT107600.12733714248115002No Hit
TATTGCACTTGTCCCGGCCTGTAAT106000.12544365337362362No Hit
CACTAGATTGTGAGCTCCTGGAA105290.12460341758215877No Hit
AACATTCAACGCTGTCGGTGA104820.12404720515682291No Hit
ACTGGACTTGGAGTCAGAAGGCA103820.12286377446461892No Hit
CAAAGTGCTGTTCGTGCAGGT102390.1211714685747672No Hit
AGCAGCATTGTACAGGGCTATGT101950.12065075907019744No Hit
CCCCCCACTGCTAAATTTGACTGT100440.11886377872496941No Hit
TAGCTTATCAGACTGATGTTGACA100050.11840224075500985No Hit
ATCCCACCGCTGCCACCA99740.1180353772404266No Hit
AATGACACGATCACTCCCGTTG96150.11378686105541426No Hit
ACCACAGGGTAGAACCACGGAC95980.11358567783773957No Hit
TGTCAGTTTGTCAAATACCCCA94160.1114318339779283No Hit
AGTTCTACAGTCCGACGATC93970.11120698214640956No Hit
TCGTACGACTCTTAGCGG92070.10895846383122194No Hit
ACTGGACTTGGAGTCAGAAGG90620.10724248932752617No Hit
AGCAGCATTGTACAGGGCTATG90110.10663893967450212No Hit
TATTGCACTTGTCCCGGCCTGA88560.10480462210158592No Hit
TTCACAGTGGCTAAGTTCCG88230.10441408997315861No Hit
TGAGGTAGTAGGTTGTATAGT85980.10175137091569962No Hit
GGAATACCGGGTGCTGTAGGCT85350.1010058095796111No Hit
CCCCCCACTGCTAAATTTGACTGTA85060.10066261467887194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)