FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7060559
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82584711.69662345431856No Hit
CAACGGAATCCCAAAAGCAGCTG80129611.348903110929319No Hit
AAGCTGCCAGTTGAAGAACTGT2567283.6360860379468534No Hit
TTCAAGTAATCCAGGATAGGCT2332513.3035769547425353No Hit
AACATTCAACGCTGTCGGTGAGT2324643.2924305285176434No Hit
CCCCCACTGCTAAATTTGACTG2268333.2126776364307696No Hit
TAGCAGCACGTAAATATTGGCG2163323.0639500356841434No Hit
AACATTCAACGCTGTCGGTGAG1625922.3028204990568026No Hit
TATTGCACTTGTCCCGGCCTGTAA1123621.5914037401287915No Hit
TATTGCACTTGTCCCGGCCTGTAT1103041.5622559063666206No Hit
TATTGCACTTGTCCCGGCCTGT825631.1693550043275611No Hit
TGAGGTAGTAGGTTGTATAGTT806211.141850099970838No Hit
TAGCTTATCAGACTGATGTTGACT733101.0383030578740295No Hit
TAGCTTATCAGACTGATGTTGAC650820.9217683755634646No Hit
TAGCTTATCAGACTGATGTTGA586240.8303025298705102No Hit
TATTGCACTTGTCCCGGCCTGTA490670.6949449753199428No Hit
CAACGGAATCCCAAAAGCAGCTGT453700.642583682113555No Hit
TGAGGTAGTAGATTGTATAGTT440020.6232084456768933No Hit
TGTAAACATCCTTGACTGGAAGCT401750.5690059384816415No Hit
TTCAAGTAATTCAGGATAGGTT386580.547520387550051No Hit
ACTGGACTTGGAGTCAGAAGGC375590.5319550477518848No Hit
AGCAGCATTGTACAGGGCTATGA346580.49086764943115696No Hit
TGTAAACATCCCCGACTGGAAGCT324570.4596944802812355No Hit
CACCCGGCTGTGTGCACATGTGC276400.39147042040155744No Hit
CATAAAGTAGAAAGCACTACT267720.3791767762297575No Hit
GGAATACCGGGTGCTGTAGGCTTT260330.3687101828622918No Hit
CATTGCACTTGTCTCGGTCTGA247340.3503122061581809No Hit
CAACGGAATCCCAAAAGCAGCT240490.3406104247553204No Hit
CTCCCACTGCTTCACTTGACTAG229030.32437941528425723No Hit
CCCCCACTGCTAAATTTGACTGGCTT224840.3184450409663031No Hit
TGAGAACTGAATTCCATGGGTT222020.3144510229289211No Hit
TTAATGCTAATCGTGATAGGGGTT221660.313941148285851No Hit
TTCTCACTACTGCACTTGACTAG194720.27578552916277593No Hit
GGAATACCGGGTGCTGTAGGCTT186500.2641433914793432No Hit
ACCCCCCACTGCTAAATTTGACTG186200.26371849594345154No Hit
CTTCTCACTACTGCACTTGACTAG186110.263591027282684No Hit
TGAGGTAGTAGTTTGTACAGTT183660.26012104707290173No Hit
CCCATAAAGTAGAAAGCACTAC179890.254781526505196No Hit
CCCATAAAGTAGAAAGCACT172590.24444240179849783No Hit
TGAGAACTGAATTCCATAGGCTGT168270.23832390608165727No Hit
AACGGAATCCCAAAAGCAGCTG166390.23566122739006926No Hit
TAGCAGCACGTAAATATTGGC163230.23118566107867664No Hit
CCCCCCACTGCTAAATTTGACTGG160370.22713499030317572No Hit
TCGTACGACTCTTAGCGG150630.21334004857122502No Hit
TATTGCACTTGTCCCGGCCTGTT149780.21213617788619854No Hit
AACCGGGCGGAAACACCA147970.2095726414863186No Hit
CCCCCCACTGCTAAATTTGACTGGCT138150.1956643942781301No Hit
TGAGGTAGTAGTTTGTGCTGTT136900.19389399621191467No Hit
TGATGTAGTAGGTTGTATAGTT135000.1912029911512672No Hit
AACATTCAACGCTGTCGGTGAGTT134320.190239894603246No Hit
CTCCCACTGCTTCACTTGACTAGCCT131060.18562269644655613No Hit
AGCTCGGTCTGAGGCCCCTCAGT130960.1854810646012589No Hit
TATTGCACTTGTCCCGGCCTGTAG130750.18518363772613472No Hit
CACTAGATTGTGAGCTCCTGGA126970.17982995397389923No Hit
TTCAAGTAATCCAGGATAGGCTT124770.17671405337736007No Hit
ACTGGACTTGGAGTCAGAAGGCT122980.17417884334653955No Hit
CAAAGAATTCTCCTTTTGGGCT122200.17307411495322114No Hit
CAACACCAGTCGATGGGCTGT121210.1716719596847785No Hit
TGAATACCGGGTGCTGTAGGCTTT113450.1606813284897131No Hit
TTCAAGTAATCCAGGATAGGC109130.1545628327728725No Hit
TGTAAACATCCTACACTCTCAGCT107120.15171603268239808No Hit
CACTAGATTGTGAGCTCCTGGAA105430.14932245449687484No Hit
TCGTTTCCCGGCCAATGCACCA105020.14874176393115615No Hit
CGCCCCACTGCTAAATTTGACTG100800.14276490005961284No Hit
TGTAAACATCCCCGACTGGAAGC100300.14205674083312667No Hit
TATTGCACTTGTCCCGGCCTGTAAT98940.14013054773708428No Hit
TATTGCACTTGTCCCGGCCTGTTT98060.13888418749846862No Hit
CGCCTGTCTGAGCGTCGCT95900.13582493964004833No Hit
ACTGGACTTGGAGTCAGAAGGCA95660.13548502321133496No Hit
ACTTGACTTGGAGTCAGAAGGC93060.13180259523360685No Hit
TAGCTTATCAGACTGATGTTGACA91350.12938069067902414No Hit
AATGCACCCGGGCAAGGATTCT90320.12792188267246263No Hit
CAAAGAATTCTCCTTTTGGGCTT89160.1262789532670147No Hit
TGAGGTAGTAGGTTGTGTGGTT87460.12387121189696171No Hit
ATCCCGGACGAGCCCCCA86580.12262485165834604No Hit
CAACGGAATCCCAAAAGCAGCTGA85140.12058535308606584No Hit
CTACGCCTGTCTGAGCGTCGCT82280.11653468231056492No Hit
TCGTTTCCCGGGCGGCGCACCA81020.11475012105981977No Hit
TGAATACCGGGTGCTGTAGGCTT80540.11407028820239304No Hit
GACCCGCGGGCGCTCTCCAGTCCT79180.11214409510635064No Hit
CCCCCCACTGCTAAATTTGACTGT78330.11094022442132415No Hit
CCCGCCACTGCTAAATTTGACTG75310.10666294269334765No Hit
TATTGCACTTGTCCCGGCCTGTAAA74870.10603976257403981No Hit
TGAGGTAGTAGGTTGTATAGT74360.10531744016302391No Hit
TGATGTAGTAGATTGTATAGTT74120.10497752373431056No Hit
CCCCCCACTGCTAAATTTGACTGTA73610.10425520132329466No Hit
AGCAGCATTGTACAGGGCTATGT72440.102598108733317No Hit
TATTGCACTTGTCCCGGCCTGA71970.10193243906042No Hit
ATGCACCTGGGCAAGGATTCTGA71770.10164917536982553No Hit
ACCACAGGGTAGAACCACGGAC71370.10108264798863659No Hit
TTCACAGTGGCTAAGTTCTGC71290.1009693425123988No Hit
CGACGGAATCCCAAAAGCAGCTG70960.10050195742291794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)