FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9215794
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7955188.632115691822104No Hit
CCCCCCACTGCTAAATTTGACTG7165827.7755861296378805No Hit
AACATTCAACGCTGTCGGTGAGT4978415.4020413216701675No Hit
AACATTCAACGCTGTCGGTGAG3491193.7882682707534476No Hit
CCCCCACTGCTAAATTTGACTG3242993.518948014680016No Hit
AAGCTGCCAGTTGAAGAACTGT2575112.7942356350413213No Hit
TTCAAGTAATCCAGGATAGGCT2051962.226568866448187No Hit
GCCTGTCTGAGCGTCGCT1869502.0285826701421494No Hit
TAGCAGCACGTAAATATTGGCG1693981.8381270241066585No Hit
TGAGGTAGTAGGTTGTATAGTT1569231.7027615851656408No Hit
TATTGCACTTGTCCCGGCCTGTAA1521381.6508398516720317No Hit
TAGCTTATCAGACTGATGTTGACT1372141.488900468044316No Hit
TATTGCACTTGTCCCGGCCTGTAT1285011.394356254056894No Hit
TAGCTTATCAGACTGATGTTGAC1204141.306604726624749No Hit
TATTGCACTTGTCCCGGCCTGT1100761.1944277400297794No Hit
TAGCTTATCAGACTGATGTTGA959421.041060596623579No Hit
TGAGGTAGTAGATTGTATAGTT911880.9894752421766372No Hit
TCCTGTCTGAGCGTCGCT800480.8685958041162813No Hit
TATTGCACTTGTCCCGGCCTGTA726580.7884073797656501No Hit
AGCAGCATTGTACAGGGCTATGA676870.7344673719920389No Hit
GGAATACCGGGTGCTGTAGGCTTT630760.6844337015345612No Hit
ACTGGACTTGGAGTCAGAAGGC626650.6799739664319754No Hit
TGTAAACATCCCCGACTGGAAGCT570730.6192955267880337No Hit
TCGTACGACTCTTAGCGG557670.6051242030800602No Hit
TGTAAACATCCTTGACTGGAAGCT524270.5688820735359319No Hit
CGCCTGTCTGAGCGTCGCT464970.5045360171896204No Hit
CAACACCAGTCGATGGGCTGT446180.4841471065868009No Hit
CATTGCACTTGTCTCGGTCTGA445780.48371306910723044No Hit
TTCAAGTAATTCAGGATAGGTT437240.4744463689184025No Hit
TGAGGTAGTAGTTTGTACAGTT422110.45802890125365214No Hit
CATAAAGTAGAAAGCACTACT418460.45406830925257224No Hit
GGAATACCGGGTGCTGTAGGCTT416200.45161599749299947No Hit
CAACGGAATCCCAAAAGCAGCTGT382140.41465770610758007No Hit
TTAATGCTAATCGTGATAGGGGTT362850.39372624865529765No Hit
TGATGTAGTAGGTTGTATAGTT332350.3606308908380548No Hit
CCCATAAAGTAGAAAGCACT320590.34787018893868504No Hit
CACCCGGCTGTGTGCACATGTGC316860.3438227894416911No Hit
AACATTCAACGCTGTCGGTGAGTT288130.31264804747154723No Hit
TGAGAACTGAATTCCATAGGCTGT285670.30997871697218926No Hit
TGAATACCGGGTGCTGTAGGCTTT270090.29307295714292225No Hit
TGAGGTAGTAGTTTGTGCTGTT237980.2582305984704085No Hit
ACTGGACTTGGAGTCAGAAGGCT232810.25262066404696115No Hit
CAACGGAATCCCAAAAGCAGCT232560.2523493906222296No Hit
TGTCAGTTTGTCAAATACCCCAA231140.25080855756975473No Hit
TGAGAACTGAATTCCATGGGTT207420.22507013503123008No Hit
CCCATAAAGTAGAAAGCACTAC202970.2202414680710094No Hit
AACGGAATCCCAAAAGCAGCTG198460.21534769548885316No Hit
TATTGCACTTGTCCCGGCCTGTAG190100.20627631216583184No Hit
TGATGTAGTAGATTGTATAGTT189910.2060701443630359No Hit
TATTGCACTTGTCCCGGCCTGTT179750.19504559238194777No Hit
TGAATACCGGGTGCTGTAGGCTT179420.1946875114613022No Hit
AACCGGGCGGAAACACCA167740.18201361705784655No Hit
ATCCCGGACGAGCCCCCA165810.1799193862189194No Hit
ACTGGACTTGGAGTCAGAAGGCA164550.17855216815827266No Hit
TGAGGTAGTAGGTTGTGTGGTT162290.17609985639869988No Hit
TTCAAGTAATCCAGGATAGGCTT153610.16668124309202223No Hit
AGCAGCATTGTACAGGGCTATGT150870.16370808635696502No Hit
ACCACAGGGTAGAACCACGGAC150470.1632740488773946No Hit
TCGTTTCCCGGCCAATGCACCA148480.16111471241653186No Hit
TTATAAAGCAATGAGACTGATT146170.15860814597201284No Hit
ACTTGACTTGGAGTCAGAAGGC143330.155526479867063No Hit
TAGCAGCACGTAAATATTGGC142770.15491882739566445No Hit
TAGCTTATCAGACTGATGTTGACA141610.15366011870491028No Hit
TGAGGTAGTAGGTTGTATAGT140200.15213013658942465No Hit
CACTAGATTGTGAGCTCCTGGA133090.1444151203900608No Hit
ACCGGGTGCTGTAGGCTT132440.1437098094857589No Hit
AACATTCAACGCTGTCGGTGA132340.1436013001158663No Hit
TGTCAGTTTGTCAAATACCCCA130910.14204961612640213No Hit
AGCTCGGTCTGAGGCCCCTCAGT130210.14129005053715393No Hit
ACTGGACTTGGAGTCAGAAGG124870.13549565018488913No Hit
TATTGCACTTGTCCCGGCCTGTAAT124050.1346058733517698No Hit
TGTAAACATCCTACACTCTCAGCT119190.12933231797498945No Hit
AGCAGCATTGTACAGGGCTATG117540.12754191337176157No Hit
CCCCCACTGCTAAATTTGACTGGCTT114790.12455790569971509No Hit
ATCCCACCGCTGCCACCA113770.12345111012681056No Hit
TTCTCACTACTGCACTTGACTAG113330.12297366889928313No Hit
ACCCCCCACTGCTAAATTTGACTG108290.11750479665669611No Hit
AATGCACCCGGGCAAGGATTCT107450.11659331794959826No Hit
TGAGGTAGTAAGTTGTATTGTT107150.11626778983992046No Hit
TGTAAACATCCCCGACTGGAAGC106470.1155299261246508No Hit
CACTAGATTGTGAGCTCCTGGAA105130.11407590056808996No Hit
AACATTCAACGCTGTCGGTGAGA101210.10982233326830004No Hit
TATTGCACTTGTCCCGGCCTGTAAA100880.10946425234765447No Hit
CACCCGTAGAACCGACCTTGCG98230.10658875404550058No Hit
TTCAAGTAATCCAGGATAGGC96970.10522153598485383No Hit
TCCTGTACTGAGCTGCCCCGAGT94340.10236773955667845No Hit
CAAAGAATTCTCCTTTTGGGCT93810.10179263989624768No Hit
TTCACAGTGGCTAAGTTCTG93700.10167327958936583No Hit
TTCACAGTGGCTAAGTTCCGC93020.10093541587409614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)