FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10491109
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9831569.371325757839328No Hit
CCCCCCACTGCTAAATTTGACTG6246125.953727103588381No Hit
AAGCTGCCAGTTGAAGAACTGT5423155.169281912903583No Hit
TTCAAGTAATCCAGGATAGGCT3822153.6432278036573638No Hit
TAGCAGCACGTAAATATTGGCG3780893.6038992636526794No Hit
AACATTCAACGCTGTCGGTGAGT3392103.2333092716890084No Hit
CCCCCACTGCTAAATTTGACTG2008811.9147737384103052No Hit
AACATTCAACGCTGTCGGTGAG1911301.8218283691457213No Hit
TAGCTTATCAGACTGATGTTGAC1430481.3635164785724752No Hit
TATTGCACTTGTCCCGGCCTGT1226011.168618112727644No Hit
TATTGCACTTGTCCCGGCCTGTAA1192181.1363717601256456No Hit
TAGCTTATCAGACTGATGTTGACT1154921.1008559724238876No Hit
TATTGCACTTGTCCCGGCCTGTAT1100191.0486879890390997No Hit
TGAGGTAGTAGGTTGTATAGTT1084801.034018424553591No Hit
TTAATGCTAATCGTGATAGGGGTT1083751.0330175770740728No Hit
TAGCTTATCAGACTGATGTTGA1028550.9804015952936911No Hit
AGCAGCATTGTACAGGGCTATGA856080.8160052478722698No Hit
GCCTGTCTGAGCGTCGCT836500.797341825349446No Hit
TGTAAACATCCTTGACTGGAAGCT833300.7942916235071049No Hit
TATTGCACTTGTCCCGGCCTGTA694530.662017714237837No Hit
CAACGGAATCCCAAAAGCAGCTGT660250.6293424270017594No Hit
CATAAAGTAGAAAGCACTACT616050.5872115140544245No Hit
TTCAAGTAATTCAGGATAGGTT553680.5277611737710475No Hit
ACTGGACTTGGAGTCAGAAGGC535510.5104417464350052No Hit
TGAGGTAGTAGATTGTATAGTT534090.5090882193674663No Hit
TGTAAACATCCCCGACTGGAAGCT517610.4933796798794103No Hit
GGAATACCGGGTGCTGTAGGCTTT463240.4415548442018856No Hit
CGCCTGTCTGAGCGTCGCT423360.4035417037417112No Hit
CCCATAAAGTAGAAAGCACTAC417550.3980036810217109No Hit
TGATGTAGTAGGTTGTATAGTT401040.38226654589138287No Hit
CATTGCACTTGTCTCGGTCTGA384670.36666285709165736No Hit
TGTAAACATCCTACACTCTCAGCT330390.3149238083409485No Hit
GGAATACCGGGTGCTGTAGGCTT322170.3070886023584351No Hit
AACATTCAACGCTGTCGGTGAGTT304200.28995981263753906No Hit
TCCTGTCTGAGCGTCGCT276790.26383292748173715No Hit
CAACGGAATCCCAAAAGCAGCT276740.26378526807795055No Hit
CCCATAAAGTAGAAAGCACT274070.2612402559157473No Hit
AGCTCGGTCTGAGGCCCCTCAGT265350.25292845589536816No Hit
CCCCCACTGCTAAATTTGACTGGCTT254760.242834194173371No Hit
TGAGGTAGTAGTTTGTACAGTT254740.24281513041185637No Hit
TTCACAGTGGCTAAGTTCCG248200.236581280396572No Hit
AACCGGGCGGAAACACCA235510.22448532371553853No Hit
TATTGCACTTGTCCCGGCCTGTT227040.2164118207140923No Hit
ACTTGACTTGGAGTCAGAAGGC225830.21525846314245708No Hit
TAGCAGCACGTAAATATTGGC225080.21454357208565844No Hit
TGAGGTAGTAGTTTGTGCTGTT216950.2067941530299609No Hit
CTACGCCTGTCTGAGCGTCGCT213550.2035533135724736No Hit
CACCCGGCTGTGTGCACATGTGC213370.2033817397188419No Hit
CAAAGAATTCTCCTTTTGGGCT208770.19899707457047677No Hit
TGATGTAGTAGATTGTATAGTT199350.19001804289708552No Hit
AAGCTGCCAGTTGAAGCACTGT192030.18304070618273055No Hit
TTCAAGTAATCCAGGATAGGCTT191370.1824116020527477No Hit
TTCTCACTACTGCACTTGACTAG188860.1800190999826615No Hit
TTCAAGTAATCCAGGATAGGC188510.17968548415615546No Hit
TGAATACCGGGTGCTGTAGGCTTT188500.17967595227539815No Hit
TGAGAACTGAATTCCATAGGCTGT182880.17431903528978682No Hit
CCCCCCACTGCTAAATTTGACTGGCT181900.17338491097556988No Hit
CTCCCACTGCTTCACTTGACTAG176770.168495056147067No Hit
TAGCTTATCAGACTGATGTTGACA176270.16801846210920124No Hit
AACGGAATCCCAAAAGCAGCTG165520.15777169029508703No Hit
CAACACCAGTCGATGGGCTGT162400.15479774349880457No Hit
CTTCTCACTACTGCACTTGACTAG159320.15186192422555136No Hit
CCCCCCACTGCTAAATTTGACTGG158070.15067043913088693No Hit
AGCAGCATTGTACAGGGCTATGT155950.14864968041033602No Hit
AGCAGCATTGTACAGGGCTATG155720.14843044715291778No Hit
TGCAAGTAATCCAGGATAGGCT151830.144722545538322No Hit
TGTAAACATCCTACACTCAGCT151080.14400765448152336No Hit
GCGTTCGCGCTTTCCCCTG149220.14223472466066267No Hit
TTAATGCTAATCGTGATAGGGGT149020.14204408704551635No Hit
TACGCCTGTCTGAGCGTCGCT148410.1414626423193201No Hit
TGAGGTAGTAGGTTGTGTGGTT148370.14142451479629084No Hit
ACCACAGGGTAGAACCACGGAC143280.13657278749081722No Hit
TGAGAACTGAATTCCATGGGTT141160.13455202877026634No Hit
CAAAGTGCTGTTCGTGCAGGTAG140260.13369415950210792No Hit
ACGCCTGTCTGAGCGTCGCT139910.13336054367560188No Hit
TAGCAGCACGTAAATATTGGCGT139130.13261705697653126No Hit
ACCCCCCACTGCTAAATTTGACTG138860.13235969619608376No Hit
CACTAGATTGTGAGCTCCTGGA137590.13114914733990465No Hit
AGCTACATTGTCTGCTGGGTTT137120.13070114894431084No Hit
TGTAAACATCCCCGACTGGAAGC136890.13048191568689257No Hit
CAAAGTGCTGTTCGTGCAGGT135540.129195111784655No Hit
ATCCCGGACGAGCCCCCA134160.12787971224014544No Hit
TGAATACCGGGTGCTGTAGGCTT132130.12594474044641038No Hit
TTAATGCTAATCGTGATAGGGGTTT123280.11750902597618612No Hit
TTCACAGTGGCTAAGTTCTGC117980.11245712917480888No Hit
TCGTTTCCCGGCCAATGCACCA117970.11244759729405157No Hit
TATTGCACTTGTCCCGGCCTGTAG117250.11176130187952485No Hit
CACTAGATTGTGAGCTCCTGGAA116250.1108081138037933No Hit
CAAAGAATTCTCCTTTTGGGCTT115910.11048402985804456No Hit
ATCACATTGCCAGGGATTTCC113860.10852999430279488No Hit
TGAGGTAGTAGGTTGTATAGT111230.10602310966362088No Hit
ACTGGACTTGGAGTCAGAAGGCT109690.10455520002699428No Hit
TTCACAGTGGCTAAGTTCTG109060.10395469153928341No Hit
TATTGCACTTGTCCCGGCCTGTTT105120.10019913052090108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)