FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12553903
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7670766.110259096314509No Hit
CAACGGAATCCCAAAAGCAGCTG7175625.715847892085832No Hit
AACATTCAACGCTGTCGGTGAGT3207972.5553566886728376No Hit
TAGCAGCACGTAAATATTGGCG3005932.3944186919398693No Hit
TTCAAGTAATCCAGGATAGGCT2518012.005758687158886No Hit
AAGCTGCCAGTTGAAGAACTGT2476861.9729800365671137No Hit
CCCCCACTGCTAAATTTGACTG1940781.5459574604009607No Hit
TAGCTTATCAGACTGATGTTGAC1759851.4018349512498225No Hit
TAGCTTATCAGACTGATGTTGACT1643911.309481202778132No Hit
AACATTCAACGCTGTCGGTGAG1610601.2829476219467364No Hit
TATTGCACTTGTCCCGGCCTGTAA1439351.1465358621936141No Hit
TATTGCACTTGTCCCGGCCTGTAT1228360.9784686085275631No Hit
TAGCTTATCAGACTGATGTTGA983170.7831588311619104No Hit
TGTAAACATCCTTGACTGGAAGCT803450.6400001656855243No Hit
AGCAGCATTGTACAGGGCTATGA737850.5877455003435983No Hit
TATTGCACTTGTCCCGGCCTGT708790.5645973208491415No Hit
CCCCCACTGCTAAATTTGACTGGCTT674470.5372592093470852No Hit
TGTAAACATCCCCGACTGGAAGCT627470.4998206533856443No Hit
ACTGGACTTGGAGTCAGAAGGC575920.4587577265811278No Hit
TATTGCACTTGTCCCGGCCTGTA573750.4570291804867379No Hit
CAACGGAATCCCAAAAGCAGCTGT498740.3972788383023192No Hit
TGAGGTAGTAGGTTGTATAGTT478040.3807899423788761No Hit
CCCCCCACTGCTAAATTTGACTGGCT459600.366101283401664No Hit
TTCAAGTAATTCAGGATAGGTT453750.36144137803199533No Hit
GGAATACCGGGTGCTGTAGGCTTT451550.3596889349869917No Hit
AACATTCAACGCTGTCGGTGAGTT334600.26653065584464053No Hit
CAAAGAATTCTCCTTTTGGGCT300280.23919254434258413No Hit
GCCTGTCTGAGCGTCGCT299680.23871460533031041No Hit
TAGCTTATCAGACTGATGTTGACA296910.23650812022364678No Hit
CATAAAGTAGAAAGCACTACT289390.23051795126981628No Hit
CTTCTCACTACTGCACTTGACTAG288720.2299842527061106No Hit
CAACGGAATCCCAAAAGCAGCT279900.22295854922568703No Hit
GGAATACCGGGTGCTGTAGGCTT276490.2202422625059314No Hit
CTCCCACTGCTTCACTTGACTAG276210.2200192243002037No Hit
TGAGGTAGTAGATTGTATAGTT270990.2158611548934224No Hit
ACTTGACTTGGAGTCAGAAGGC266590.21235626880341515No Hit
CCCATAAAGTAGAAAGCACTAC261620.20839734065174792No Hit
TTTAAACATCCTTGACTGGAAGCT248160.1976755754764076No Hit
CCCCCCACTGCTAAATTTGACTGG247870.1974445716204753No Hit
TGAATACCGGGTGCTGTAGGCTTT247270.1969666326082016No Hit
CTCCCACTGCTTCACTTGACTAGCCT246720.1965285218469507No Hit
TTCTCACTACTGCACTTGACTAG237920.18951874966693624No Hit
TGTAAACATCCTACACTCTCAGCT227450.18117871390275997No Hit
TTCAAGTAATCCAGGATAGGCTT207680.16543062344834114No Hit
CAAAGAATTCTCCTTTTGGGCTT204340.16277009628001746No Hit
CGCCCCACTGCTAAATTTGACTG202540.16133627924319632No Hit
CATTGCACTTGTCTCGGTCTGA202200.1610654471362412No Hit
CACCCGGCTGTGTGCACATGTGC195200.1554894919930479No Hit
TAGCAGCACGTAAATATTGGC193250.15393619020315832No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC192640.15345028554068005No Hit
ACCCCCCACTGCTAAATTTGACTG191550.1525820296683828No Hit
TTTAAACATCCCCGACTGGAAGCT191070.15219967845856386No Hit
GTCAGGATGGCCGAGCGGTCTAAGG183450.1461298530026877No Hit
TCCTGTCTGAGCGTCGCT177240.14118318422565476No Hit
CGCCTGTCTGAGCGTCGCT176210.14036272225458488No Hit
TATTGCACTTGTCCCGGCCTGTT167630.1335281943790708No Hit
CCCCCACTGCTAAATTTGACTGGCTA160580.12791241098485467No Hit
TATTGCACTTGTCCCGGCCTGTAG159100.1267334947545795No Hit
TGTAAACATCCCCGACTGGAAGC157700.12561830372594085No Hit
TGATGTAGTAGGTTGTATAGTT154800.1233082651666179No Hit
AGCTCGGTCTGAGGCCCCTCAGT154750.1232684369155951No Hit
CTACGCCTGTCTGAGCGTCGCT154430.12301353610904911No Hit
TGAATACCGGGTGCTGTAGGCTT150200.11964406607251944No Hit
AGCAGCATTGTACAGGGCTATGT149430.11903071100676818No Hit
CCCCCACTGCTAAATTTGACTGGCT144540.11513550805673742No Hit
CACTAGATTGTGAGCTCCTGGAA141460.11268208779373234No Hit
CCCGCCACTGCTAAATTTGACTG139930.1114633433124344No Hit
TCGTTTCCCGGCCAATGCACCA139760.11132792725895684No Hit
TTAATGCTAATCGTGATAGGGGTT133220.10611839202517336No Hit
CCCATAAAGTAGAAAGCACT132860.10583162861780913No Hit
TATTGCACTTGTCCCGGCCTGTAAT131900.10506692619817119No Hit
CTACGGGGATGATTTTACGAACTGAA131160.10447746808303361No Hit
AACCGGGCGGAAACACCA130520.10396766646994166No Hit
TCGTACGACTCTTAGCGG128980.10274095633843915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)