FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812738
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG95564410.843894371987457No Hit
CAACGGAATCCCAAAAGCAGCTG8298969.417005248539104No Hit
AACATTCAACGCTGTCGGTGAGT3258373.697341280314926No Hit
CCCCCACTGCTAAATTTGACTG2791683.1677782773072343No Hit
TTCAAGTAATCCAGGATAGGCT2780703.1553190393269377No Hit
TAGCAGCACGTAAATATTGGCG2570442.916732575052157No Hit
AACATTCAACGCTGTCGGTGAG2209002.5065989707171594No Hit
AAGCTGCCAGTTGAAGAACTGT2062352.34019211736466No Hit
TATTGCACTTGTCCCGGCCTGTAA1549371.7581028733635335No Hit
TATTGCACTTGTCCCGGCCTGTAT1370071.5546473751971295No Hit
TAGCTTATCAGACTGATGTTGACT1288521.4621108672469327No Hit
TAGCTTATCAGACTGATGTTGAC1116881.2673473329174203No Hit
TAGCTTATCAGACTGATGTTGA922751.0470639204297234No Hit
TGAGGTAGTAGGTTGTATAGTT897691.0186278089737832No Hit
TATTGCACTTGTCCCGGCCTGT743150.8432680059250599No Hit
TTCAAGTAATTCAGGATAGGTT609280.6913628885824133No Hit
TGTAAACATCCTTGACTGGAAGCT562710.6385189256732698No Hit
TTAATGCTAATCGTGATAGGGGTT557120.6321758345703685No Hit
TGAGGTAGTAGATTGTATAGTT536550.6088346209770448No Hit
TATTGCACTTGTCCCGGCCTGTA530470.6019355165216531No Hit
CAACGGAATCCCAAAAGCAGCTGT467820.5308452378818025No Hit
AGCAGCATTGTACAGGGCTATGA457850.5195320682403131No Hit
TGTAAACATCCCCGACTGGAAGCT440780.5001623785933498No Hit
CCCCCACTGCTAAATTTGACTGGCTT438130.4971553676053912No Hit
ACTGGACTTGGAGTCAGAAGGC382350.43386062311168216No Hit
GGAATACCGGGTGCTGTAGGCTTT368590.4182468603968483No Hit
TTCTCACTACTGCACTTGACTAG366960.4163972649589719No Hit
CATAAAGTAGAAAGCACTACT330990.3755813460016626No Hit
ACCCCCCACTGCTAAATTTGACTG295060.3348108158894546No Hit
CTTCTCACTACTGCACTTGACTAG286760.3253926305309428No Hit
CTCCCACTGCTTCACTTGACTAG248860.28238669979749764No Hit
TGATGTAGTAGGTTGTATAGTT246170.2793342999644378No Hit
CAACGGAATCCCAAAAGCAGCT242150.2747727210317611No Hit
CCCCCCACTGCTAAATTTGACTGGCT235030.26669350660373653No Hit
GGAATACCGGGTGCTGTAGGCTT233310.26474178626438233No Hit
CCCATAAAGTAGAAAGCACTAC232500.26382266215108174No Hit
CCCCCCACTGCTAAATTTGACTGG215600.24464587509579883No Hit
TGAGAACTGAATTCCATGGGTT203290.23067745801588566No Hit
CATTGCACTTGTCTCGGTCTGA198930.22573007389984817No Hit
CCCCCCACTGCTAAATTTGACTGT198210.22491307468802546No Hit
CTCCCACTGCTTCACTTGACTAGCCT195410.22173585553093714No Hit
TAGCAGCACGTAAATATTGGC190760.21645940228791552No Hit
AACATTCAACGCTGTCGGTGAGTT190190.21581261124522252No Hit
TAGCTTATCAGACTGATGTTGACA189980.2155743198084409No Hit
TGAGGTAGTAGTTTGTACAGTT186920.21210207315819443No Hit
TCGTACGACTCTTAGCGG186530.21165953191845713No Hit
CAAAGAATTCTCCTTTTGGGCT185630.21063828290367875No Hit
AGCTCGGTCTGAGGCCCCTCAGT182680.20729085557746074No Hit
TATTGCACTTGTCCCGGCCTGTAG182020.2065419396332899No Hit
CCCCCCACTGCTAAATTTGACTGTA177370.20126548639026826No Hit
CCCATAAAGTAGAAAGCACT163310.1853113073371749No Hit
TGAATACCGGGTGCTGTAGGCTTT149830.1700152665380498No Hit
TGTAAACATCCTACACTCTCAGCT147990.1679273796633918No Hit
AACGGAATCCCAAAAGCAGCTG147740.1676436993815089No Hit
TGATGTAGTAGATTGTATAGTT147360.1672125053530469No Hit
TTCAAGTAATCCAGGATAGGCTT146460.16619125633826853No Hit
CACCCGGCTGTGTGCACATGTGC145840.165487729239199No Hit
TCGTTTCCCGGCCAATGCACCA144700.16419414715381303No Hit
TATTGCACTTGTCCCGGCCTGTT138910.15762411182540545No Hit
TATTGCACTTGTCCCGGCCTGTAAT134910.15308522731527932No Hit
CAAAGAATTCTCCTTTTGGGCTT133200.1511448541872004No Hit
TGTAAACATCCCCGACTGGAAGC125690.14262309851943855No Hit
TATTGCACTTGTCCCGGCCTGTAAA123650.14030826741927424No Hit
CAACACCAGTCGATGGGCTGT120800.13707431220580937No Hit
TTCAAGTAATCCAGGATAGGC120780.13705161778325875No Hit
GGGAATACCGGGTGCTGTAGGCTTT120280.13648425721949298No Hit
TGAGGTAGTAGTTTGTGCTGTT117480.13330703806240465No Hit
ACTGGACTTGGAGTCAGAAGGCT117140.13292123287904395No Hit
TCGTTTCCCGGGCGGCGCACCA116240.13189998386426557No Hit
TATTGCACTTGTCCCGGCCTGTTT115660.13124184561029728No Hit
ACTTGACTTGGAGTCAGAAGGC115360.13090142927203782No Hit
AACCGGGCGGAAACACCA112320.12745187704434194No Hit
CAACGGAATCCCAAAAGCAGCTGA103930.11793156678435238No Hit
TGAGGTAGTAGGTTGTGTGGTT102450.11625217951560571No Hit
ACTGGACTTGGAGTCAGAAGGCA101410.1150720695429729No Hit
CCCGCCACTGCTAAATTTGACTG99590.11300687709086552No Hit
ATCCCGGACGAGCCCCCA99470.11287071055556172No Hit
CCCCCACTGCTAAATTTGACTGGCT99240.11260972469622947No Hit
TGAGAACTGAATTCCATAGGCTGT97480.11061261551177398No Hit
CGCCTGTCTGAGCGTCGCT97090.11017007427203668No Hit
AACATTCAACGCTGTCGGTGAGA95990.108921881031752No Hit
CCCCCACTGCTAAATTTGACTGGCTA94210.10690207742474586No Hit
AACATTCAACGCTGTCGGTGA92860.1053702039025783No Hit
TGTCAGTTTGTCAAATACCCCAA92450.10490496824029036No Hit
GGGAATACCGGGTGCTGTAGGCTT92200.10462128795840747No Hit
TGAATACCGGGTGCTGTAGGCTT90870.10311210885879053No Hit
TTCACAGTGGCTAAGTTCTGC90610.10281708136563235No Hit
ATGCACCTGGGCAAGGATTCTGA89000.10099018035030656No Hit
CACCCGTAGAACCGACCTTGCG88960.10094479150520531No Hit
AGCAGCATTGTACAGGGCTATGT88780.10074054170224962No Hit
ACCGGGTGCTGTAGGCTT88580.10051359747674334No Hit
CTACGCCTGTCTGAGCGTCGCT88280.10017318113848388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)