FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8521099
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103767212.177678020170871No Hit
CAACGGAATCCCAAAAGCAGCTG7311498.580454234835202No Hit
CCCCCACTGCTAAATTTGACTG3694844.3361073495332No Hit
AACATTCAACGCTGTCGGTGAGT2971713.487472683981256No Hit
AACATTCAACGCTGTCGGTGAG2262462.6551269971162172No Hit
TTCAAGTAATCCAGGATAGGCT2192372.5728723489775205No Hit
TAGCTTATCAGACTGATGTTGACT1804162.1172855754873874No Hit
TAGCAGCACGTAAATATTGGCG1735732.036979032868882No Hit
TATTGCACTTGTCCCGGCCTGTAA1684731.9771275982123901No Hit
AAGCTGCCAGTTGAAGAACTGT1657081.9446787321682333No Hit
TAGCTTATCAGACTGATGTTGAC1603281.8815413363933455No Hit
TATTGCACTTGTCCCGGCCTGTAT1434371.6833157319261283No Hit
TAGCTTATCAGACTGATGTTGA1298451.5238057907788654No Hit
TGAGGTAGTAGGTTGTATAGTT1189611.3960757878766579No Hit
TATTGCACTTGTCCCGGCCTGT665720.7812607270494101No Hit
TCGTACGACTCTTAGCGG651720.7648309214574317No Hit
TGAGGTAGTAGATTGTATAGTT611420.7175365525033801No Hit
TATTGCACTTGTCCCGGCCTGTA575510.675394101159956No Hit
TTAATGCTAATCGTGATAGGGGTT493010.578575603921513No Hit
AGCAGCATTGTACAGGGCTATGA473500.555679496271549No Hit
TTCAAGTAATTCAGGATAGGTT469480.5509617949515667No Hit
TGTAAACATCCTTGACTGGAAGCT425350.4991727006105668No Hit
GCCTGTCTGAGCGTCGCT398190.4672988777621291No Hit
ACTGGACTTGGAGTCAGAAGGC380540.4465855871408136No Hit
CAACGGAATCCCAAAAGCAGCTGT370290.434556622332401No Hit
CATAAAGTAGAAAGCACTACT362100.42494518606109377No Hit
GGAATACCGGGTGCTGTAGGCTTT348650.40916083711737183No Hit
TGTAAACATCCCCGACTGGAAGCT346630.40679025088195786No Hit
CAACGGAATCCCAAAAGCAGCT322330.3782728026044528No Hit
CCCCCACTGCTAAATTTGACTGGCTT262670.3082583596317799No Hit
CATTGCACTTGTCTCGGTCTGA261760.3071904222683013No Hit
TAGCTTATCAGACTGATGTTGACA248400.29151169350338496No Hit
ACCCCCCACTGCTAAATTTGACTG232840.27325113814544344No Hit
CCCATAAAGTAGAAAGCACTAC215470.2528664436359676No Hit
CAACACCAGTCGATGGGCTGT215460.2528547080605448No Hit
CACCCGTAGAACCGACCTTGCG209560.2459307185610682No Hit
TATTGCACTTGTCCCGGCCTGTAG203660.23900672906159173No Hit
CACCCGGCTGTGTGCACATGTGC201480.23644837361941223No Hit
GGAATACCGGGTGCTGTAGGCTT200830.23568556121692755No Hit
TCGTTTCCCGGCCAATGCACCA194390.22812785064461757No Hit
CCCCCCACTGCTAAATTTGACTGT184440.2164509530988902No Hit
CCCATAAAGTAGAAAGCACT177550.20836514163255232No Hit
AACATTCAACGCTGTCGGTGAGTT171750.2015585078873042No Hit
TTCTCACTACTGCACTTGACTAG168910.1982256044672172No Hit
TGAGGTAGTAGTTTGTACAGTT167600.19668824408682495No Hit
TATTGCACTTGTCCCGGCCTGTAAT158820.18638440886557003No Hit
TGATGTAGTAGGTTGTATAGTT156740.183943409177619No Hit
CTCCCACTGCTTCACTTGACTAG153190.17977727990251025No Hit
CCCCCCACTGCTAAATTTGACTGTA152790.17930785688559656No Hit
TAGCAGCACGTAAATATTGGC152210.17862719351107176No Hit
TGAGAACTGAATTCCATGGGTT151760.17809909261704387No Hit
AACGGAATCCCAAAAGCAGCTG150590.17672603029257142No Hit
CAAAGAATTCTCCTTTTGGGCT147370.17294717500641643No Hit
TTCAAGTAATCCAGGATAGGCTT141400.16594103647898No Hit
TATTGCACTTGTCCCGGCCTGTAAA140990.16545987788664349No Hit
CTTCTCACTACTGCACTTGACTAG139100.16324185413172643No Hit
CGCCCCACTGCTAAATTTGACTG135190.15865324414139537No Hit
CCCCCCACTGCTAAATTTGACTGG132090.1550152157603145No Hit
TATTGCACTTGTCCCGGCCTGTT129350.1517996680944559No Hit
ACCGGGTGCTGTAGGCTT126200.1481029618362608No Hit
CTCCCACTGCTTCACTTGACTAGCCT118520.13909003991151844No Hit
TGAGAACTGAATTCCATAGGCTGT116800.1370715209387897No Hit
TGAGGTAGTAGTTTGTGCTGTT116300.13648474216764764No Hit
CCCCCCACTGCTAAATTTGACTGGCT114380.13423151168646202No Hit
AGCTCGGTCTGAGGCCCCTCAGT113470.13316357432298345No Hit
ACCGGGTGCTGTAGGCTTT111510.1308634015401065No Hit
TGTAAACATCCTACACTCTCAGCT110890.13013579586389032No Hit
ACCGGGTGCTGTAGGCTTA107270.12588751756082167No Hit
CACTAGATTGTGAGCTCCTGGA105970.12436189275585227No Hit
AGCAGCATTGTACAGGGCTATGT104820.12301230158222548No Hit
CAAAGAATTCTCCTTTTGGGCTT103520.12148667677725608No Hit
TCCTGTCTGAGCGTCGCT99450.11671029758015955No Hit
CAACGGAATCCCAAAAGCAGCTGA99040.11622913898782306No Hit
TTCACAGTGGCTAAGTTCTG98820.11597095632852053No Hit
TATTGCACTTGTCCCGGCCTGTTT97430.11433971134474556No Hit
CCCGCCACTGCTAAATTTGACTG97230.11410499983628873No Hit
CACTAGATTGTGAGCTCCTGGAA96990.11382334602614051No Hit
TTCAAGTAATCCAGGATAGGC94950.11142928863988083No Hit
TTATAAAGCAATGAGACTGATT92970.10910564470615822No Hit
TGAGGTAGTAAGTTGTATTGTT91320.10716927476138935No Hit
AATGCACCCGGGCAAGGATTCT90870.10664117386736148No Hit
GCCCCCACTGCTAAATTTGACTG90410.10610133739791076No Hit
TGAGGTAGTAGGTTGTGTGGTT89930.10553802977761437No Hit
TGAATACCGGGTGCTGTAGGCTTT89340.10484563082766671No Hit
ACTGGACTTGGAGTCAGAAGGCT89020.10447009241413578No Hit
TTAGGTAGTAGGTTGTATAGTT88770.10417670302856473No Hit
ATGCACCTGGGCAAGGATTCTGA87850.10309703008966331No Hit
TTCACAGTGGCTAAGTTCTGC85440.10026875641275848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)