FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9092999
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6267906.893105344012465No Hit
CAACGGAATCCCAAAAGCAGCTG6252106.875729338582354No Hit
AAGCTGCCAGTTGAAGAACTGT3363403.6988896622555436No Hit
TTCAAGTAATCCAGGATAGGCT3065323.371077023103159No Hit
TAGCAGCACGTAAATATTGGCG3049843.354052936770366No Hit
AACATTCAACGCTGTCGGTGAGT2775183.051996376552994No Hit
CCCCCACTGCTAAATTTGACTG1987392.185626546313268No Hit
TAGCTTATCAGACTGATGTTGAC1809901.9904324194910832No Hit
AACATTCAACGCTGTCGGTGAG1437771.5811835017247884No Hit
TAGCTTATCAGACTGATGTTGACT1345401.4795998547893825No Hit
TAGCTTATCAGACTGATGTTGA1263181.3891786417220546No Hit
GCCTGTCTGAGCGTCGCT1074981.1822062226114838No Hit
TTAATGCTAATCGTGATAGGGGTT949711.0444408934829972No Hit
TATTGCACTTGTCCCGGCCTGTAA923711.0158474668258513No Hit
TGAGGTAGTAGGTTGTATAGTT896470.9858903536665955No Hit
TATTGCACTTGTCCCGGCCTGTAT861680.9476301493049762No Hit
TGTAAACATCCTTGACTGGAAGCT787240.8657649692912096No Hit
AGCAGCATTGTACAGGGCTATGA704170.7744089711216288No Hit
TATTGCACTTGTCCCGGCCTGT696940.7664577990165841No Hit
CATAAAGTAGAAAGCACTACT619850.6816782889781469No Hit
TTCAAGTAATTCAGGATAGGTT606320.6667987096446398No Hit
GGAATACCGGGTGCTGTAGGCTTT555130.6105026515454361No Hit
TGAGGTAGTAGATTGTATAGTT518310.5700099604102014No Hit
TATTGCACTTGTCCCGGCCTGTA510910.561871831284706No Hit
TCCTGTCTGAGCGTCGCT479650.5274937344653837No Hit
TGTAAACATCCCCGACTGGAAGCT460200.5061036518314804No Hit
AACCGGGCGGAAACACCA432200.47531073081609265No Hit
CAACGGAATCCCAAAAGCAGCTGT407980.44867485413778224No Hit
TTCTCACTACTGCACTTGACTAG391610.4306719928155716No Hit
CCCATAAAGTAGAAAGCACTAC386400.4249423100123512No Hit
GGAATACCGGGTGCTGTAGGCTT344410.3787639259610608No Hit
ACTGGACTTGGAGTCAGAAGGC339920.37382606112680755No Hit
CGCCTGTCTGAGCGTCGCT337320.37096671846109297No Hit
CCCCCACTGCTAAATTTGACTGGCTT311490.3425602488243978No Hit
TGAATACCGGGTGCTGTAGGCTTT305490.3359617657496718No Hit
TGTAAACATCCTACACTCTCAGCT296310.3258660866453411No Hit
TGATGTAGTAGGTTGTATAGTT292560.3217420347236374No Hit
TTCACAGTGGCTAAGTTCCG258430.28420766350023796No Hit
AACATTCAACGCTGTCGGTGAGTT257440.28311891379290816No Hit
TAGCTTATCAGACTGATGTTGACA253030.2782690287329846No Hit
CTTCTCACTACTGCACTTGACTAG252160.2773122486871493No Hit
CAAAGAATTCTCCTTTTGGGCT242930.26716158222386255No Hit
CTACGCCTGTCTGAGCGTCGCT238380.26215773255886204No Hit
AGCTCGGTCTGAGGCCCCTCAGT228930.25176512171616866No Hit
TGAGGTAGTAGTTTGTACAGTT215220.23668758789041985No Hit
CCCCCCACTGCTAAATTTGACTGGCT208520.22931928179030925No Hit
CATTGCACTTGTCTCGGTCTGA201840.221972970633781No Hit
TGAATACCGGGTGCTGTAGGCTT190310.20929288565851595No Hit
CTCCCACTGCTTCACTTGACTAG183580.20189158714303168No Hit
TAGCAGCACGTAAATATTGGC183120.201385703440636No Hit
CCCCCCACTGCTAAATTTGACTGG180370.19836139869805328No Hit
CAACGGAATCCCAAAAGCAGCT173480.19078414063390967No Hit
TGATGTAGTAGATTGTATAGTT164700.18112836040122737No Hit
ACCCCCCACTGCTAAATTTGACTG159410.1753106978236773No Hit
CCCATAAAGTAGAAAGCACT156310.17190148156840224No Hit
TACGCCTGTCTGAGCGTCGCT155740.17127462567630328No Hit
TGAGGTAGTAGTTTGTGCTGTT153720.16905313637447889No Hit
TTCAAGTAATCCAGGATAGGCTT150170.16514903388859936No Hit
CAAAGAATTCTCCTTTTGGGCTT145680.1602111690543461No Hit
CAACACCAGTCGATGGGCTGT140900.15495437753814775No Hit
TATTGCACTTGTCCCGGCCTGTT139900.15385463035902677No Hit
TTCAAGTAATCCAGGATAGGC135940.14949963152970763No Hit
TGTAAACATCCCCGACTGGAAGC132470.1456835088181578No Hit
CCCCCCACTGCTAAATTTGACTGTA130990.14405588299305874No Hit
TCGTTTCCCGGCCAATGCACCA130590.14361598412141033No Hit
AGCAGCATTGTACAGGGCTATGT130070.1430441155882674No Hit
ACGCCTGTCTGAGCGTCGCT125290.13778732407206906No Hit
TGTAAACATCCTACACTCAGCT124580.1370065035748932No Hit
TAGCAGCACGTAAATATTGGCGT122930.13519192072934352No Hit
AGCTACATCTGGCTACTGGGTCTC122700.1349389788781457No Hit
CTCCCACTGCTTCACTTGACTAGCCT121530.13365227467857416No Hit
ACCGGGTGCTGTAGGCTT120840.13289344912498066No Hit
AGCAGCATTGTACAGGGCTATG118650.13048500280270567No Hit
TTAATGCTAATCGTGATAGGGGTTT117660.12939625309537592No Hit
ACTTGACTTGGAGTCAGAAGGC116670.1283075033880461No Hit
AGCTACATTGTCTGCTGGGTTT114680.12611900650159535No Hit
TATTGCACTTGTCCCGGCCTGTAG113810.12516222645576008No Hit
TGTCAGTTTGTCAAATACCCCA111070.12214891918496858No Hit
TTCACAGTGGCTAAGTTCTGC110340.12134610374421025No Hit
ATCCCGGACGAGCCCCCA109640.12057628071882555No Hit
ACCGGGTGCTGTAGGCTTT107900.11866272062715502No Hit
TGTCAGTTTGTCAAATACCCCAA106620.11725504423788016No Hit
AACGGAATCCCAAAAGCAGCTG105400.11591335267935254No Hit
CCCCCCACTGCTAAATTTGACTGT104220.11461565100798977No Hit
TGAGGTAGTAGGTTGTGTGGTT103680.11402178753126445No Hit
GGGCTACGCCTGTCTGAGCGTCGCT103550.1138788203979787No Hit
CCCCCACTGCTAAATTTGACTGGCT102050.11222919962929723No Hit
TTAATGCTAATCGTGATAGGGGT100290.11029364459404427No Hit
CAAAGTGCTGTTCGTGCAGGTAG99540.10946883420970353No Hit
ACTGGACTTGGAGTCAGAAGGCA98600.1084350718613298No Hit
CAAAGTGCTGTTCGTGCAGGT97770.10752228170265937No Hit
ATCACATTGCCAGGGATTTCCA96900.10656550165682412No Hit
TTATAAAGCAATGAGACTGATT95270.1047729137548569No Hit
AACATTCAACGCTGTCGGTGAGA93650.10299132332468089No Hit
TCGTACGACTCTTAGCGG92190.10138569244316424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)