FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604102
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT3181056.909164914243863No Hit
CCCCCCACTGCTAAATTTGACTG2319225.0372906595032No Hit
CAACGGAATCCCAAAAGCAGCTG1916154.161832209625243No Hit
AACATTCAACGCTGTCGGTGAGT1631873.5443828134129087No Hit
TCCTGTCTGAGCGTCGCT1241462.6964215823194184No Hit
TTCAAGTAATCCAGGATAGGCT1215192.639363767353547No Hit
AAGCTGCCAGTTGAAGAACTGT1136292.4679948445972744No Hit
TAGCAGCACGTAAATATTGGCG1014592.203665340168398No Hit
CGCCTGTCTGAGCGTCGCT880741.9129463248207794No Hit
CCCCCACTGCTAAATTTGACTG872651.8953750373036913No Hit
AACATTCAACGCTGTCGGTGAG750281.629590308815921No Hit
TAGCTTATCAGACTGATGTTGAC503911.0944805306224754No Hit
TAGCTTATCAGACTGATGTTGA448730.9746308835034497No Hit
TAGCTTATCAGACTGATGTTGACT417310.9063873910699632No Hit
TGAGGTAGTAGGTTGTATAGTT397830.8640772945516845No Hit
TATTGCACTTGTCCCGGCCTGT389560.8461150513172819No Hit
CATAAAGTAGAAAGCACTACT363200.7888617584927528No Hit
TATTGCACTTGTCCCGGCCTGTAA329720.7161439950722204No Hit
TATTGCACTTGTCCCGGCCTGTAT286330.621901947437307No Hit
AGCAGCATTGTACAGGGCTATGA248550.5398446863253682No Hit
ACGCCTGTCTGAGCGTCGCT237420.5156705911380765No Hit
TACGCCTGTCTGAGCGTCGCT221730.4815922844454793No Hit
CTACGCCTGTCTGAGCGTCGCT217620.4726654622334605No Hit
TATTGCACTTGTCCCGGCCTGTA198050.4301598878565244No Hit
TGTAAACATCCTTGACTGGAAGCT194850.4232095639931522No Hit
ACTGGACTTGGAGTCAGAAGGC189270.4110899367563968No Hit
GGAATACCGGGTGCTGTAGGCTTT187530.4073106981556881No Hit
ACCGGGTGCTGTAGGCTT172150.37390570408735513No Hit
TGAGGTAGTAGATTGTATAGTT171080.37158168954554005No Hit
AACCGGGCGGAAACACCA166180.36093900612975127No Hit
TTCAAGTAATTCAGGATAGGTT146720.31867234913561865No Hit
CCCATAAAGTAGAAAGCACTAC139720.3034685156844918No Hit
GGAATACCGGGTGCTGTAGGCTT137290.2981906135007435No Hit
TGTAAACATCCCCGACTGGAAGCT136280.2959969175313666No Hit
AACATTCAACGCTGTCGGTGAGTT132790.2884167205678762No Hit
GCGTTCGCGCTTTCCCCTG131820.2863099036467915No Hit
CTCCCACTGCTTCACTTGACTAG130660.28379041124631904No Hit
TTCTCACTACTGCACTTGACTAG127320.27653601071392425No Hit
TCGTACGACTCTTAGCGG125860.2733649254512606No Hit
CAACGGAATCCCAAAAGCAGCTGT124620.27067167495420386No Hit
CCCCCACTGCTAAATTTGACTGGCTT116440.2529049095784585No Hit
CCCTGTCTGAGCGTCGCT111640.24247942378340012No Hit
ACCGGGTGCTGTAGGCTTT109640.23813547136879243No Hit
TTCACAGTGGCTAAGTTCCG107180.23279240989882502No Hit
CAACACCAGTCGATGGGCTGT106730.2318150206055383No Hit
ATCCCGGACGAGCCCCCA104330.22660227770800906No Hit
TCCACCACGTTCCCGTGG103450.2246909386455817No Hit
CATTGCACTTGTCTCGGTCTGA100630.21856596574098489No Hit
AGCTCGGTCTGAGGCCCCTCAGT95250.20688073374569024No Hit
CCCCCCACTGCTAAATTTGACTGGCT91660.1990833391614695No Hit
CAACGGAATCCCAAAAGCAGCT91430.1985837846337896No Hit
CCCATAAAGTAGAAAGCACT89980.19543441913319903No Hit
TGTAAACATCCTACACTCTCAGCT86220.18726778859373663No Hit
CTTCTCACTACTGCACTTGACTAG84400.18331479189644365No Hit
TGATGTAGTAGGTTGTATAGTT83620.18162065045474665No Hit
TCGTTTCCCGGCCAATGCACCA81200.17636446803307138No Hit
TGAATACCGGGTGCTGTAGGCTTT80970.1758649135053915No Hit
TAGCAGCACATCATGGTTTACA80300.17440968944649793No Hit
CCCCCCACTGCTAAATTTGACTGG80270.17434453016027882No Hit
GCTTGACTAGCTTGCTGTTT80190.1741707720636945No Hit
TGAGGTAGTAGGTTGTGTGGTT75950.16496159294472626No Hit
GTCAGGATGGCCGAGCGGTCTAAGG75200.16333261078924835No Hit
ACCGGGTGCTGTAGGCTTA74350.1614864310130401No Hit
TCAGGCTCAGTCCCCTCCCGAT70730.15362387714260023No Hit
TGAGGTAGTAGTTTGTACAGTT67550.14671699280337402No Hit
TTCACAGTGGCTAAGTTCCGC67210.14597852089289073No Hit
TTCAAGTAATCCAGGATAGGC63880.13874584012256896No Hit
TATTGCACTTGTCCCGGCCTGTT63030.1368996603463607No Hit
TACCTGGTTGATCCTGCC63010.1368562208222146No Hit
CCTCGACACAAGGGTTTGT62450.13563991414612447No Hit
TGAATACCGGGTGCTGTAGGCTT61570.1337285750836971No Hit
TCGTTCGCGCTTTCCCCTG60520.13144800006602808No Hit
TTCAAGTAATCCAGGATAGGCTT59220.1286244309965331No Hit
TTCACAGTGGCTAAGTTCTGC56800.12336824857485781No Hit
TAGCAGCACGTAAATATTGGC56790.12334652881278478No Hit
TAGCTTATCAGACTGATGTTGACA56360.12241257904364412No Hit
CCAAGTCCTTCTGATCGAGGCCC55990.12160894784694172No Hit
CAAAGTGCTGTTCGTGCAGGT54390.11813378591525557No Hit
AGCAGCATTGTACAGGGCTATGT54070.11743875352891835No Hit
TGAGGTAGTAGTTTGTGCTGTT52910.11491926112844589No Hit
AGCAGCATTGTACAGGGCTATG52840.11476722279393461No Hit
CTTGACTAGCTTGCTGTTT50980.11072734704834951No Hit
ACCACAGGGTAGAACCACGGAC50660.11003231466201226No Hit
ACCTGTCTGAGCGTCGCT48170.10462409390582571No Hit
CCCCCACTGCTAAATTTGACTGGCT46920.10190912364669592No Hit
TTATAAAGCAATGAGACTGATT46920.10190912364669592No Hit
TTCACAGTGGCTAAGTTCTG46500.10099689363962831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)