FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5475528
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG3902447.12705697057891No Hit
CAACGGAATCCCAAAAGCAGCTG3871887.071245001395299No Hit
AACATTCAACGCTGTCGGTGAGT2460434.493502727042944No Hit
TTCAAGTAATCCAGGATAGGCT1889453.450717446792346No Hit
TAGCAGCACGTAAATATTGGCG1808583.3030239275554796No Hit
AAGCTGCCAGTTGAAGAACTGT1415782.585650187525294No Hit
AACATTCAACGCTGTCGGTGAG1312552.397120423820315No Hit
CCCCCACTGCTAAATTTGACTG1263432.307412180158699No Hit
TGAGGTAGTAGGTTGTATAGTT870331.5894905477608734No Hit
TATTGCACTTGTCCCGGCCTGT756311.3812549218997694No Hit
GGAATACCGGGTGCTGTAGGCTTT746561.363448419951464No Hit
TATTGCACTTGTCCCGGCCTGTAA708681.294267876997433No Hit
TATTGCACTTGTCCCGGCCTGTAT689901.2599698147831588No Hit
TAGCTTATCAGACTGATGTTGAC548101.0009993556785757No Hit
GGAATACCGGGTGCTGTAGGCTT546870.998752996971251No Hit
TGAGGTAGTAGATTGTATAGTT541880.989639720589503No Hit
TAGCTTATCAGACTGATGTTGA518690.9472876405709185No Hit
TAGCTTATCAGACTGATGTTGACT502680.9180484512178553No Hit
GCCTGTCTGAGCGTCGCT389790.7118765532748623No Hit
TTCAAGTAATTCAGGATAGGTT381430.6966086192966231No Hit
TGTAAACATCCTTGACTGGAAGCT375300.6854133519178425No Hit
CATAAAGTAGAAAGCACTACT348460.6363952480929693No Hit
TATTGCACTTGTCCCGGCCTGTA347660.6349342017792623No Hit
AGCAGCATTGTACAGGGCTATGA306360.5595076858341332No Hit
CGCCTGTCTGAGCGTCGCT300080.5480384722715326No Hit
TGTAAACATCCCCGACTGGAAGCT290940.531346018137429No Hit
ACTGGACTTGGAGTCAGAAGGC270280.49361449708594307No Hit
TTCTCACTACTGCACTTGACTAG262750.47986239865817504No Hit
ACCGGGTGCTGTAGGCTT251480.45927990871382635No Hit
TGAGGTAGTAGTTTGTACAGTT230320.4206352337162736No Hit
CAACGGAATCCCAAAAGCAGCTGT215200.3930214583872094No Hit
CTCCCACTGCTTCACTTGACTAG211800.3868120115539542No Hit
CTTCTCACTACTGCACTTGACTAG208010.37989030464276685No Hit
CACCCGGCTGTGTGCACATGTGC207760.3794337276697334No Hit
CATTGCACTTGTCTCGGTCTGA202030.3689689834478063No Hit
ACCGGGTGCTGTAGGCTTT177910.3249184370895373No Hit
CCCCCCACTGCTAAATTTGACTGG176430.32221550140917915No Hit
CGCGACCTCAGATCAGACGTGGCG173850.31750362704747376No Hit
TGAGGTAGTAGTTTGTGCTGTT159920.2920631581100489No Hit
AACATTCAACGCTGTCGGTGAGTT158260.2890314870091067No Hit
ATCCCGGACGAGCCCCCA152320.2781832181298315No Hit
CCCATAAAGTAGAAAGCACT146800.2681019985652525No Hit
CAACACCAGTCGATGGGCTGT144850.26454069817559145No Hit
ACCGGGTGCTGTAGGCTTA139430.25464210940022586No Hit
CGCGACCTCAGATCAGACGTGG137170.2505146535640033No Hit
CCCATAAAGTAGAAAGCACTAC125890.22991390054073324No Hit
AACGGAATCCCAAAAGCAGCTG121060.22109283342172664No Hit
TGAGGTAGTAGGTTGTGTGGTT110700.20217228365921971No Hit
CAACGGAATCCCAAAAGCAGCT109990.20087560505580465No Hit
TATTGCACTTGTCCCGGCCTGTT108870.19883014021661471No Hit
GGAATACCGGGTGCTGTAGGCTTA100930.18432925555307178No Hit
TGTAAACATCCTACACTCTCAGCT99230.18122453213644418No Hit
TACGCCTGTCTGAGCGTCGCT98590.1800556950854785No Hit
AGCTCGGTCTGAGGCCCCTCAGT98380.1796721704281304No Hit
TAGCAGCACGTAAATATTGGC97450.1779737040884459No Hit
CTACGCCTGTCTGAGCGTCGCT97050.17724318093159233No Hit
TGTAAACATCCCCGACTGGAAGC95670.17472287604044762No Hit
CCCCCCACTGCTAAATTTGACTGGCT94130.17191036188656145No Hit
TGAGGTAGTAGGTTGTATAGT94060.17178252033411207No Hit
TCGTACGACTCTTAGCGG92070.16814816762876567No Hit
TTCAAGTAATCCAGGATAGGCTT91990.16800206299739495No Hit
CGCGACCTCAGATCAGACGT89480.16341803018813894No Hit
AACATTCAACGCTGTCGGTGA89060.1626509808734427No Hit
CGCGACCTCAGATCAGACGTG85480.15611279861960345No Hit
AACCGGGCGGAAACACCA84690.15467001538481767No Hit
CGCGACCTCAGATCAGACGTGGC84460.1542499645696269No Hit
ACCCCCCACTGCTAAATTTGACTG84180.1537385983598294No Hit
CACTAGATTGTGAGCTCCTGGA82980.15154702888926877No Hit
AATGACACGATCACTCCCGTTG81400.14866146241969724No Hit
TGAGAACTGAATTCCATGGGTT80900.1477483084736303No Hit
GGAATACCGGGTGCTGTAGGCT80490.14699952223785542No Hit
TTCAAGTAATCCAGGATAGGC78660.14365737879525042No Hit
TTATAAAGCAATGAGACTGATT78290.1429816448751609No Hit
TACCACCCTGAACGCGCCCGA78170.14276248792810484No Hit
ACGCCTGTCTGAGCGTCGCT77010.14064397077322954No Hit
TATTGCACTTGTCCCGGCCTGTAG76760.14018739380019607No Hit
AGTTCTACAGTCCGACGATC76390.13951165988010655No Hit
CAAAGTGCTGTTCGTGCAGGT75950.13870808440756763No Hit
CCCCCACTGCTAAATTTGACTGGCTT75290.13750272119875928No Hit
GAATACCGGGTGCTGTAGGCTTT74250.13560336099094006No Hit
CTCCCACTGCTTCACTTGACTAGCCT73200.1336857377041995No Hit
ATCCCACCGCTGCCACCA68510.12512035369009164No Hit
GGGAATACCGGGTGCTGTAGGCTT67840.12389672740236192No Hit
CAAAGAATTCTCCTTTTGGGCTT67060.12247220724649752No Hit
GTTCTACAGTCCGACGATC66540.12152252714258789Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCGTTTCCCGGCCAATGCACCA66390.12124858095876781No Hit
ACTGGACTTGGAGTCAGAAGGCT66090.12070068859112765No Hit
CAAAGAATTCTCCTTTTGGGCT65340.11933095767202725No Hit
TGTCAGTTTGTCAAATACCCCAA62370.11390682323238965No Hit
ACTGGACTTGGAGTCAGAAGGCA61770.11281103849710933No Hit
TATTGCACTTGTCCCGGCCTGA61180.11173351684075034No Hit
AGAGGTAGTAGGTTGCATAGTT61120.11162393836722231No Hit
TAGCTTATCAGACTGATGTTGACA60710.11087515213144741No Hit
AACATTCAACGCTGTCGGTGAGA60520.11052815363194197No Hit
ACTGGACTTGGAGTCAGAAGG60000.10957847352803236No Hit
TTCACAGTGGCTAAGTTCTGC59510.10868358266088676No Hit
TGAGGTAGTAGATTGTATAGT58820.10742343021531439No Hit
TTCACAGTGGCTAAGTTCCG58780.10735037789962903No Hit
ACCACAGGGTAGAACCACGGAC58580.10698511632120226No Hit
TGTCAGTTTGTCAAATACCCCA57840.1056336484810232No Hit
CACTAGATTGTGAGCTCCTGGAA57610.10521359766583241No Hit
GGGAATACCGGGTGCTGTAGGCTTT56580.10333250053693452No Hit
CCCCCACTGCTAAATTTGACTGG56550.10327771130017051No Hit
CCACCCTGAACGCGCCCGA55730.10178013882862072No Hit
TTCACAGTGGCTAAGTTCCGC55360.1011044049085312No Hit
AGCAGCATTGTACAGGGCTATG55330.10104961567176719No Hit
TATTGCACTTGTCCCGGCCTGTTT54870.1002095140413856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)