FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620190
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5442327.141974150250848No Hit
CCCCCCACTGCTAAATTTGACTG4366485.730145836258676No Hit
AACATTCAACGCTGTCGGTGAGT2812493.6908397297180255No Hit
TTCAAGTAATCCAGGATAGGCT2293623.009924949377903No Hit
TAGCAGCACGTAAATATTGGCG2203132.891174629503989No Hit
AAGCTGCCAGTTGAAGAACTGT2010992.6390286856364473No Hit
AACATTCAACGCTGTCGGTGAG1799012.360846645556082No Hit
CCCCCACTGCTAAATTTGACTG1453121.9069340790715192No Hit
GGAATACCGGGTGCTGTAGGCTTT1331411.7472136521530304No Hit
GGAATACCGGGTGCTGTAGGCTT944511.2394835299382299No Hit
TATTGCACTTGTCCCGGCCTGT932701.2239852287147694No Hit
TATTGCACTTGTCCCGGCCTGTAA907991.1915582157400275No Hit
TGAGGTAGTAGGTTGTATAGTT860611.1293812883930716No Hit
TATTGCACTTGTCCCGGCCTGTAT852501.118738509144785No Hit
TAGCTTATCAGACTGATGTTGAC740540.9718130387824976No Hit
TAGCTTATCAGACTGATGTTGACT671830.8816446834002827No Hit
TAGCTTATCAGACTGATGTTGA662290.8691253105237533No Hit
TTCAAGTAATTCAGGATAGGTT551490.7237221119158447No Hit
TGAATACCGGGTGCTGTAGGCTTT540740.7096148521231098No Hit
TGAGGTAGTAGATTGTATAGTT519020.6811116258256028No Hit
TATTGCACTTGTCCCGGCCTGTA449460.5898278126923344No Hit
TGTAAACATCCTTGACTGGAAGCT446110.5854315968499473No Hit
CATAAAGTAGAAAGCACTACT430590.5650646506189478No Hit
TGAATACCGGGTGCTGTAGGCTT385400.5057616673600002No Hit
AGCAGCATTGTACAGGGCTATGA381070.5000793943458103No Hit
CGCCTGTCTGAGCGTCGCT347950.45661591115182165No Hit
TGTAAACATCCCCGACTGGAAGCT324260.4255274474783437No Hit
TTAATGCTAATCGTGATAGGGGTT316790.4157245423014387No Hit
TGAGAACTGAATTCCATGGGTT293350.3849641544370941No Hit
CACCCGGCTGTGTGCACATGTGC285710.37493815770997835No Hit
CAACGGAATCCCAAAAGCAGCTGT278490.3654633283422067No Hit
TTCTCACTACTGCACTTGACTAG272440.35752389376117916No Hit
ACTGGACTTGGAGTCAGAAGGC268420.35224843475031464No Hit
CATTGCACTTGTCTCGGTCTGA261770.34352161822736704No Hit
ACCGGGTGCTGTAGGCTT234640.3079188314202139No Hit
TGATGTAGTAGGTTGTATAGTT227310.29829964869642356No Hit
CTCCCACTGCTTCACTTGACTAG226210.2968561151362368No Hit
TCGTACGACTCTTAGCGG220570.289454724882188No Hit
ATCCCGGACGAGCCCCCA219910.2885886047460759No Hit
CTTCTCACTACTGCACTTGACTAG214800.2818827352073899No Hit
TGAGGTAGTAGTTTGTACAGTT209160.27448134495334103No Hit
CGCGACCTCAGATCAGACGTGGCG203740.26736866141132964No Hit
CCCATAAAGTAGAAAGCACT201940.26500651558556937No Hit
CAACACCAGTCGATGGGCTGT192090.2520803287057147No Hit
CCCATAAAGTAGAAAGCACTAC179730.23586026070216096No Hit
GCCTGTCTGAGCGTCGCT179020.2349285254042222No Hit
GGAATACCGGGTGCTGTAGGCTTA176270.23131969150375514No Hit
ACCGGGTGCTGTAGGCTTT174160.22855073167466952No Hit
AACATTCAACGCTGTCGGTGAGTT170050.22315716537251695No Hit
CAACGGAATCCCAAAAGCAGCT168310.2208737577409487No Hit
CCCCCCACTGCTAAATTTGACTGG163740.21487653194999073No Hit
AGCTCGGTCTGAGGCCCCTCAGT159660.20952233474493417No Hit
GGAATACCGGGTGCTGTAGGCT154540.20280334217388282No Hit
ACCGGGTGCTGTAGGCTTA151830.1992470004028771No Hit
CGCGACCTCAGATCAGACGTGG149160.19574315076133272No Hit
AACCGGGCGGAAACACCA141170.18525784790143027No Hit
TGAGGTAGTAGTTTGTGCTGTT140730.18468043447735555No Hit
TGATGTAGTAGATTGTATAGTT136770.179483713660683No Hit
TAGCAGCACGTAAATATTGGC135190.17741027454696012No Hit
TGAGAACTGAATTCCATAGGCTGT134180.1760848482780613No Hit
TATTGCACTTGTCCCGGCCTGTT133360.17500875962410387No Hit
CTACGCCTGTCTGAGCGTCGCT129860.1704156982962367No Hit
AACGGAATCCCAAAAGCAGCTG122330.16053405492513967No Hit
TTCAAGTAATCCAGGATAGGCTT120920.15868370736162749No Hit
TACCACCCTGAACGCGCCCGA120850.15859184613507013No Hit
TGTAAACATCCTACACTCTCAGCT119350.15662339128026992No Hit
CACTAGATTGTGAGCTCCTGGA111720.14661051758551952No Hit
ACCCCCCACTGCTAAATTTGACTG111620.14647928726186618No Hit
TTCAAGTAATCCAGGATAGGC106720.14004900140285215No Hit
TGTAAACATCCCCGACTGGAAGC104200.13674199724678782No Hit
TATTGCACTTGTCCCGGCCTGTAG102920.13506224910402495No Hit
CCCCCACTGCTAAATTTGACTGGCTT101480.13317253244341676No Hit
AACATTCAACGCTGTCGGTGA99110.13006237377283242No Hit
TCGTTTCCCGGCCAATGCACCA98640.12944559125166172No Hit
CAAAGTGCTGTTCGTGCAGGT98460.1292093766690857No Hit
CAAAGAATTCTCCTTTTGGGCT95130.12483940689142921No Hit
ACGCCTGTCTGAGCGTCGCT94520.1240389019171438No Hit
TGAGGTAGTAGGTTGTGTGGTT92660.12159801789719153No Hit
TTATAAAGCAATGAGACTGATT92300.12112558873203949No Hit
CTCCCACTGCTTCACTTGACTAGCCT91310.11982640852787134No Hit
CCCCCCACTGCTAAATTTGACTGGCT91260.11976079336604467No Hit
TACGCCTGTCTGAGCGTCGCT89050.1168606032133057No Hit
ACCACAGGGTAGAACCACGGAC86840.11396041306056673No Hit
TAGCTTATCAGACTGATGTTGACA86180.11309429292445464No Hit
CGCGACCTCAGATCAGACGTG85240.11186072788211318No Hit
CAAAGAATTCTCCTTTTGGGCTT84900.11141454478169178No Hit
TGAGGTAGTAGGTTGTATAGT84250.11056154767794504No Hit
CCACCCTGAACGCGCCCGA83120.10907864502066221No Hit
GAATACCGGGTGCTGTAGGCTTT81490.10693959074511265No Hit
CACTAGATTGTGAGCTCCTGGAA80980.10627031609448058No Hit
ACTTGACTTGGAGTCAGAAGGC80430.10554854931438717No Hit
TTCACAGTGGCTAAGTTCCG78500.10301580406787757No Hit
GGGAATACCGGGTGCTGTAGGCTT78280.10272709735584021No Hit
CGCGACCTCAGATCAGACGTGGC77900.1022284221259575No Hit
AATGACACGATCACTCCCGTTG77510.10171662386370944No Hit
CGCGACCTCAGATCAGACGT77420.10159851657242142No Hit
ACCACCCTGAACGCGCCCGA76310.10014185997986927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)