FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9278458
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7605428.196857710623899No Hit
CCCCCCACTGCTAAATTTGACTG7196707.756353480287349No Hit
GCCTGTCTGAGCGTCGCT3386533.6498844958936063No Hit
AACATTCAACGCTGTCGGTGAGT3061873.2999772160417176No Hit
CCCCCACTGCTAAATTTGACTG2755062.9693080466603394No Hit
AACATTCAACGCTGTCGGTGAG2396882.5832740742050024No Hit
TTCAAGTAATCCAGGATAGGCT2030652.1885640911453175No Hit
AAGCTGCCAGTTGAAGAACTGT1737771.8729081922879858No Hit
TAGCAGCACGTAAATATTGGCG1726741.8610204411120899No Hit
TCCTGTCTGAGCGTCGCT1708401.8412542256482705No Hit
TATTGCACTTGTCCCGGCCTGTAA1571541.6937512677214253No Hit
TATTGCACTTGTCCCGGCCTGTAT1372671.4794160840087869No Hit
TAGCTTATCAGACTGATGTTGAC1290091.3904142261569756No Hit
TAGCTTATCAGACTGATGTTGACT1264411.3627372134464584No Hit
TGAGGTAGTAGGTTGTATAGTT1120651.2077976750016004No Hit
TAGCTTATCAGACTGATGTTGA1036871.1175024987988307No Hit
TATTGCACTTGTCCCGGCCTGT980961.0572446412970777No Hit
TCGTACGACTCTTAGCGG761730.820966156229839No Hit
TATTGCACTTGTCCCGGCCTGTA694010.747979890624067No Hit
TGAGGTAGTAGATTGTATAGTT580350.6254810874824244No Hit
TTAATGCTAATCGTGATAGGGGTT538300.5801610569342449No Hit
AGCAGCATTGTACAGGGCTATGA499910.5387856473564896No Hit
CACCCGGCTGTGTGCACATGTGC486450.5242789265199024No Hit
TGTAAACATCCTTGACTGGAAGCT454210.4895317734908106No Hit
CATAAAGTAGAAAGCACTACT452280.487451686476352No Hit
TGAGAACTGAATTCCATAGGCTGT434780.4685907938582036No Hit
GGAATACCGGGTGCTGTAGGCTTT429040.46240442107945096No Hit
TTCAAGTAATTCAGGATAGGTT423890.45685392982325296No Hit
ACTGGACTTGGAGTCAGAAGGC418160.4506783346974249No Hit
TGTAAACATCCCCGACTGGAAGCT417300.44975145654590454No Hit
CATTGCACTTGTCTCGGTCTGA394250.42490896655457183No Hit
CAACGGAATCCCAAAAGCAGCTGT357640.3854519791974054No Hit
CAACGGAATCCCAAAAGCAGCT357450.38524720379183697No Hit
CAACACCAGTCGATGGGCTGT309390.33344980383593914No Hit
GGAATACCGGGTGCTGTAGGCTT290730.3133387034785306No Hit
TGAGAACTGAATTCCATGGGTT288510.3109460645292569No Hit
TGATGTAGTAGGTTGTATAGTT237250.2556998156374691No Hit
ACCGGGTGCTGTAGGCTT233270.2514103097734559No Hit
TGAATACCGGGTGCTGTAGGCTTT222090.23936089380369022No Hit
CACTAGATTGTGAGCTCCTGGA221360.2385741251401903No Hit
CCCATAAAGTAGAAAGCACT219490.2365587040432796No Hit
CCCATAAAGTAGAAAGCACTAC201340.21699726398502855No Hit
AACATTCAACGCTGTCGGTGAGTT199030.21450762615943297No Hit
TCGTTTCCCGGCCAATGCACCA196080.21132821854665937No Hit
TGAGGTAGTAGTTTGTACAGTT191500.20639205350716683No Hit
TATTGCACTTGTCCCGGCCTGTAG181050.19512940620090105No Hit
AACGGAATCCCAAAAGCAGCTG174560.18813470945279914No Hit
CGCGACCTCAGATCAGACGTGGCG172030.18540796326286113No Hit
CACTAGATTGTGAGCTCCTGGAA170720.18399609072973117No Hit
TATTGCACTTGTCCCGGCCTGTT169100.18225011095593685No Hit
CCCCCACTGCTAAATTTGACTGGCTT167120.18011613567685492No Hit
ACCCCCCACTGCTAAATTTGACTG165830.17872581844957428No Hit
TTCTCACTACTGCACTTGACTAG165360.17821926876211544No Hit
CTCCCACTGCTTCACTTGACTAG165040.17787438386852641No Hit
CGCGACCTCAGATCAGACGTGG161030.17355254504573928No Hit
TACCACCCTGAACGCGCCCGA159350.17174189935439702No Hit
TAGCTTATCAGACTGATGTTGACA156020.16815294093048652No Hit
ACCGGGTGCTGTAGGCTTT156000.1681313856246372No Hit
TGAGGTAGTAGTTTGTGCTGTT151320.1630874440558981No Hit
TGAATACCGGGTGCTGTAGGCTT151290.16305511109712412No Hit
TAGCAGCACGTAAATATTGGC149380.16099657938851478No Hit
CTTCTCACTACTGCACTTGACTAG146430.15781717177574117No Hit
CGCCTGTCTGAGCGTCGCT141760.1527840078599267No Hit
TTATAAAGCAATGAGACTGATT140720.15166313195576248No Hit
ACCGGGTGCTGTAGGCTTA138650.14943215780035865No Hit
CTCCCACTGCTTCACTTGACTAGCCT133630.14402177603218122No Hit
TATTGCACTTGTCCCGGCCTGTAAT126360.13618642235595613No Hit
TGTAAACATCCTACACTCTCAGCT123430.13302857004903185No Hit
TGATGTAGTAGATTGTATAGTT123250.13283457229638804No Hit
CCCCCCACTGCTAAATTTGACTGG121800.13127181262231288No Hit
ACTGGACTTGGAGTCAGAAGGCT121320.13075448528192937No Hit
TTCAAGTAATCCAGGATAGGCTT121250.13067904171145678No Hit
CAAAGAATTCTCCTTTTGGGCT121100.13051737691758694No Hit
ATCCCGGACGAGCCCCCA113150.12194914284248526No Hit
CACCCGTAGAACCGACCTTGCG111610.1202893842920882No Hit
AATGCACCCGGGCAAGGATTCT109980.11853262686536922No Hit
ACTGGACTTGGAGTCAGAAGGCA109870.118414072683198No Hit
TTAGGTAGTAGGTTGTATAGTT108560.11700220015006804No Hit
AGCAGCATTGTACAGGGCTATGT104430.11255102949218501No Hit
ACCACAGGGTAGAACCACGGAC104300.11241092000416449No Hit
TATTGCACTTGTCCCGGCCTGTAAA104180.11228158816906861No Hit
CGCCCCACTGCTAAATTTGACTG104170.11227081051614395No Hit
CCCTGTCTGAGCGTCGCT102890.11089127094178795No Hit
CAAAGAATTCTCCTTTTGGGCTT101280.10915606882091831No Hit
CCCCCCACTGCTAAATTTGACTGGCT96150.10362713287056967No Hit
TGTAAACATCCCCGACTGGAAGC93980.10128838218591925No Hit
ACTTGACTTGGAGTCAGAAGGC92810.10002739679373447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)