FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10449631
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT131212412.55665391438224No Hit
CAACGGAATCCCAAAAGCAGCTG5549925.311115770499456No Hit
TCCTGTCTGAGCGTCGCT4813514.606392321413072No Hit
CCCCCCACTGCTAAATTTGACTG4709594.5069438337104915No Hit
AAGCTGCCAGTTGAAGAACTGT2693062.577181911973734No Hit
TTCAAGTAATCCAGGATAGGCT2646762.5328741273256443No Hit
TAGCAGCACGTAAATATTGGCG2465332.359250771630118No Hit
AACATTCAACGCTGTCGGTGAGT2196332.10182541373949No Hit
CCCCCACTGCTAAATTTGACTG1427591.366163073126697No Hit
AACATTCAACGCTGTCGGTGAG1276171.2212584348672217No Hit
TAGCTTATCAGACTGATGTTGAC1170371.120010840574179No Hit
TATTGCACTTGTCCCGGCCTGT943030.9024529191509251No Hit
TGAGGTAGTAGGTTGTATAGTT862070.8249764991701621No Hit
TAGCTTATCAGACTGATGTTGA859520.8225362216139499No Hit
TTAATGCTAATCGTGATAGGGGTT847000.810554937298743No Hit
TATTGCACTTGTCCCGGCCTGTAA820240.7849463775323742No Hit
TAGCTTATCAGACTGATGTTGACT790420.7564094846985506No Hit
GGAATACCGGGTGCTGTAGGCTTT780440.7468589082236492No Hit
TATTGCACTTGTCCCGGCCTGTAT772480.7392414143619043No Hit
CGCCTGTCTGAGCGTCGCT651930.6238784891064575No Hit
TGTAAACATCCTTGACTGGAAGCT560310.5362007519691365No Hit
CATAAAGTAGAAAGCACTACT557240.533262849185775No Hit
GGAATACCGGGTGCTGTAGGCTT552840.5290521741868206No Hit
AGCAGCATTGTACAGGGCTATGA552040.5282865969142834No Hit
TATTGCACTTGTCCCGGCCTGTA541490.5181905466326994No Hit
AACCGGGCGGAAACACCA511450.4894431200489281No Hit
TTCAAGTAATTCAGGATAGGTT459270.43950834244769027No Hit
TGAGGTAGTAGATTGTATAGTT455120.4355369103464036No Hit
CAACGGAATCCCAAAAGCAGCTGT339440.3248344367375269No Hit
TGAATACCGGGTGCTGTAGGCTTT315920.3023264649249337No Hit
TGTAAACATCCCCGACTGGAAGCT312980.29951296844835956No Hit
TCCGGTCTGAGCGTCGCT308000.2947472499268156No Hit
ACTGGACTTGGAGTCAGAAGGC302560.28954132447356273No Hit
CCCATAAAGTAGAAAGCACTAC283240.2710526333417898No Hit
ACCGGGTGCTGTAGGCTT281970.269837279421637No Hit
CATTGCACTTGTCTCGGTCTGA273400.2616360328895824No Hit
CCCTGTCTGAGCGTCGCT264070.2527074879486175No Hit
GCCGGTCTGAGCGTCGCT241860.23145314891980395No Hit
TTCTCACTACTGCACTTGACTAG234880.22477348721691706No Hit
TGATGTAGTAGGTTGTATAGTT234780.2246777900578499No Hit
CTCCCACTGCTTCACTTGACTAG230050.22015131443397382No Hit
TTCACAGTGGCTAAGTTCCG227550.21775888545729508No Hit
AACATTCAACGCTGTCGGTGAGTT221220.21170125528834463No Hit
TGAATACCGGGTGCTGTAGGCTT220600.2111079329021283No Hit
CTACGCCTGTCTGAGCGTCGCT219370.2099308578456024No Hit
ATCCCGGACGAGCCCCCA217240.20789250835747214No Hit
TGTAAACATCCTACACTCTCAGCT210160.20111714949551807No Hit
CACCCGGCTGTGTGCACATGTGC208240.1992797640414288No Hit
AGCTCGGTCTGAGGCCCCTCAGT192580.18429358893151349No Hit
ACGCCTGTCTGAGCGTCGCT184350.1764177127402872No Hit
TACGCCTGTCTGAGCGTCGCT184180.17625502756987305No Hit
CCCCCACTGCTAAATTTGACTGGCTT180100.1723505834799334No Hit
TGAGGTAGTAGTTTGTACAGTT179710.17197736455957152No Hit
CTTCTCACTACTGCACTTGACTAG177000.16938397154885182No Hit
ACCGGGTGCTGTAGGCTTT176210.16862796399222135No Hit
CAACGGAATCCCAAAAGCAGCT173100.16565178234523306No Hit
GCCTGTCTGAGCGTCGCA163810.156761516267895No Hit
TATTGCACTTGTCCCGGCCTGTT162610.15561315035908924No Hit
CCCATAAAGTAGAAAGCACT162010.15503896740468637No Hit
TGAGGTAGTAGTTTGTGCTGTT161490.15454134217753718No Hit
TCGTACGACTCTTAGCGG159320.15246471382578006No Hit
CAACACCAGTCGATGGGCTGT157110.1503498066103961No Hit
CCCCCCACTGCTAAATTTGACTGGCT147820.14145954053305806No Hit
TAGCAGCACGTAAATATTGGC143060.13690435576146182No Hit
CCCCCCACTGCTAAATTTGACTGG141740.13564115326177548No Hit
TAGCTTATCAGACTGATGTTGACA137350.13144004797872766No Hit
TCGTTTCCCGGCCAATGCACCA136630.13075102843344422No Hit
CAAAGAATTCTCCTTTTGGGCT128990.1234397654807141No Hit
ACCTGTCTGAGCGTCGCT128830.12328665002620666No Hit
TTCAAGTAATCCAGGATAGGC127770.12227226014009492No Hit
GCCTGTCTGAGCGTCGCTT127380.12189904121973302No Hit
CTCCCACTGCTTCACTTGACTAGCCT126250.12081766332227425No Hit
GCGTTCGCGCTTTCCCCTG125890.12047315354963252No Hit
TGATGTAGTAGATTGTATAGTT125110.11972671570890878No Hit
ACCGGGTGCTGTAGGCTTA124860.11948747281124089No Hit
ACCCCCCACTGCTAAATTTGACTG121450.11622419968705115No Hit
GCCTGTCTGAGCGTCGCG118630.11352553980135759No Hit
CGCGACCTCAGATCAGACGTGGCG117760.1126929745174734No Hit
TTAATGCTAATCGTGATAGGGGT115220.11026226667716783No Hit
TTCAAGTAATCCAGGATAGGCTT111720.10691286610981765No Hit
GGGCTACGCCTGTCTGAGCGTCGCT111200.10641524088266849No Hit
CAAAGTGCTGTTCGTGCAGGTAG106070.1015059766225238No Hit
GGAATACCGGGTGCTGTAGGCTTA105790.1012380245771358No Hit
CAAAGTGCTGTTCGTGCAGGT105720.10117103656578877No Hit
AACGGAATCCCAAAAGCAGCTG105390.10085523594086719No Hit
AGCAGCATTGTACAGGGCTATG104630.10012793753195687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)