FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7297151
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5483267.514247683787824No Hit
AACATTCAACGCTGTCGGTGAGT3472744.7590354098469385No Hit
CCCCCCACTGCTAAATTTGACTG3117984.272873070599744No Hit
TTCAAGTAATCCAGGATAGGCT2610843.5778895078366886No Hit
TAGCAGCACGTAAATATTGGCG2544253.486634715384127No Hit
AAGCTGCCAGTTGAAGAACTGT2464993.378016982244166No Hit
AACATTCAACGCTGTCGGTGAG2144372.9386400254016944No Hit
TATTGCACTTGTCCCGGCCTGTAT1651622.26337648761825No Hit
TATTGCACTTGTCCCGGCCTGTAA1648902.2596490054817284No Hit
TGAGGTAGTAGGTTGTATAGTT1337101.8323589576260653No Hit
TAGCTTATCAGACTGATGTTGACT1210641.6590584462347018No Hit
TATTGCACTTGTCCCGGCCTGT1115441.5285965714564493No Hit
TAGCTTATCAGACTGATGTTGAC1082601.4835927062493295No Hit
CCCCCACTGCTAAATTTGACTG1055931.4470441957415985No Hit
GCCTGTCTGAGCGTCGCT1001601.37259048086027No Hit
TGAGGTAGTAGATTGTATAGTT894021.2251630807694673No Hit
TAGCTTATCAGACTGATGTTGA867551.1888886498305984No Hit
CATAAAGTAGAAAGCACTACT766631.0505880993828962No Hit
TATTGCACTTGTCCCGGCCTGTA697380.955688048664472No Hit
TGTAAACATCCTTGACTGGAAGCT684090.9374754613136003No Hit
AGCAGCATTGTACAGGGCTATGA500690.686144496667261No Hit
TTCAAGTAATTCAGGATAGGTT499520.6845411311894191No Hit
TGTAAACATCCCCGACTGGAAGCT470380.6446077380062438No Hit
ACTGGACTTGGAGTCAGAAGGC450580.6174738606889182No Hit
ATCCCGGACGAGCCCCCA438070.600330183656608No Hit
CCCATAAAGTAGAAAGCACTAC404370.554147776303382No Hit
TGAGGTAGTAGTTTGTACAGTT388810.5328243858459281No Hit
CGCCTGTCTGAGCGTCGCT377330.5170922185932565No Hit
TCGTACGACTCTTAGCGG339890.46578452330231346No Hit
CATTGCACTTGTCTCGGTCTGA338170.4634274390101013No Hit
CAACGGAATCCCAAAAGCAGCTGT318300.43619763384367405No Hit
CCCATAAAGTAGAAAGCACT315580.4324701517071526No Hit
CACCCGGCTGTGTGCACATGTGC267750.3669240228138351No Hit
ACCGGGTGCTGTAGGCTTA239470.328169171776766No Hit
CAACACCAGTCGATGGGCTGT231480.3172196930007341No Hit
AACATTCAACGCTGTCGGTGAGTT229920.3150818723636115No Hit
CACTAGATTGTGAGCTCCTGGA225050.30840803486182483No Hit
AGCTCGGTCTGAGGCCCCTCAGT222300.30463944078997407No Hit
TCCTGTCTGAGCGTCGCT216550.29675965318519515No Hit
TATTGCACTTGTCCCGGCCTGTT203600.2790130011013887No Hit
TATTGCACTTGTCCCGGCCTGTAG203270.2785607698127667No Hit
CACTAGATTGTGAGCTCCTGGAA199550.2734628898319358No Hit
TTCTCACTACTGCACTTGACTAG196950.2698998554367314No Hit
TGAGGTAGTAGTTTGTGCTGTT182680.25034427819843663No Hit
TGAGAACTGAATTCCATGGGTT182640.25028946228466425No Hit
TGTAAACATCCTACACTCTCAGCT179550.2460549329457483No Hit
TGTCAGTTTGTCAAATACCCCAA175450.24043630178407985No Hit
CTCCCACTGCTTCACTTGACTAG169210.23188501923558932No Hit
ACCGGGTGCTGTAGGCTT157500.21583766047872655No Hit
TTCAAGTAATCCAGGATAGGCTT157180.21539913316854756No Hit
AACGGAATCCCAAAAGCAGCTG152330.2087527036236471No Hit
ACCGGGTGCTGTAGGCTTT141890.1944457501290572No Hit
TATTGCACTTGTCCCGGCCTGTTT141400.19377425518534563No Hit
CTTCTCACTACTGCACTTGACTAG140580.19265052895301193No Hit
TTCAAGTAATCCAGGATAGGC134350.18411295038296452No Hit
ATCCCACCGCTGCCACCA133400.18281107243087064No Hit
TTATAAAGCAATGAGACTGATT132870.18208476157338666No Hit
TAGCAGCACGTAAATATTGGC128430.17600019514465304No Hit
TGTAAACATCCCCGACTGGAAGC128130.1755890757913602No Hit
CAAAGAATTCTCCTTTTGGGCT126430.17325939945603427No Hit
TGAGGTAGTAGGTTGTATAGT125620.17214937720214368No Hit
TAGCTTATCAGACTGATGTTGACA120720.16543442776502773No Hit
CTACGCCTGTCTGAGCGTCGCT119390.16361179863209627No Hit
CGCGACCTCAGATCAGACGTGGCG116770.16002135628000572No Hit
TATTGCACTTGTCCCGGCCTGTAAT116460.1595965329482698No Hit
TGAGGTAGTAGGTTGTGTGGTT115550.15834947090994828No Hit
ACTGGACTTGGAGTCAGAAGGCT115070.15769167994467978No Hit
CGCGACCTCAGATCAGACGTGG113280.155238667803366No Hit
ACTGGACTTGGAGTCAGAAGGCA112370.15399160576504448No Hit
TTCACAGTGGCTAAGTTCTG112340.15395049382971518No Hit
TTCACAGTGGCTAAGTTCCG111130.15229231243810085No Hit
AGCAGCATTGTACAGGGCTATGT110340.15120969814109644No Hit
CAAAGAATTCTCCTTTTGGGCTT107910.14787963137942464No Hit
TACGCCTGTCTGAGCGTCGCT107080.14674220116864786No Hit
ACCACAGGGTAGAACCACGGAC106090.1453855073027816No Hit
CCCCCACTGCTAAATTTGACTGGCTT105760.1449332760141595No Hit
TTCACAGTGGCTAAGTTCTGC105110.1440425174153584No Hit
CCCCCCACTGCTAAATTTGACTGGCT104170.1427543434417076No Hit
ACTGGACTTGGAGTCAGAAGG103290.14154839333871536No Hit
TGTCAGTTTGTCAAATACCCCA101210.13869796582255184No Hit
TGATGTAGTAGGTTGTATAGTT98810.13540901099620933No Hit
AGAGGTAGTAGGTTGCATAGTT96450.1321748720836392No Hit
TTCACAGTGGCTAAGTTCCGC94750.1298451957483133No Hit
AACATTCAACGCTGTCGGTGA94740.1298314917698702No Hit
TATTGCACTTGTCCCGGCCTGA92590.12688513640460503No Hit
TATTGCACTTGTCCCGGCCTGTAAA92550.12683032049083265No Hit
AACCGGGCGGAAACACCA91550.12545992264652328No Hit
TTCACCACCTTCTCCACCCAGC90720.1243224924357465No Hit
TGAGGTAGTAAGTTGTATTGTT90400.12388396512556751No Hit
CCCATAAAGTAGAAAGCACTA89480.12262319910880286No Hit
AACATTCAACGCTGTCGGTGAGA89390.12249986330281502No Hit
CCCCCCACTGCTAAATTTGACTGG88790.12167762459622941No Hit
TGAGGTAGTAGATTGTATAGT87470.11986869944174101No Hit
CAAAGTGCTGTTCGTGCAGGT85430.1170730878393499No Hit
CAAAGTGCTGTTCGTGCAGGTAG84810.1162234411758781No Hit
TCGTTTCCCGGGCGGCGCACCA84470.1157575059088129No Hit
AGCAGCATTGTACAGGGCTATG81920.11226299140582399No Hit
ACGCCTGTCTGAGCGTCGCT76110.10430097993038653No Hit
TGAGGGGCAGAGAGCGAGACTTT74380.1019301916597313No Hit
CAACGGAATCCCAAAAGCAGCT73600.10086128134117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)