FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8728868
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8303259.512401837214172No Hit
AACATTCAACGCTGTCGGTGAGT3906694.475597523069428No Hit
CCCCCCACTGCTAAATTTGACTG3541384.057089647821459No Hit
TTCAAGTAATCCAGGATAGGCT3305673.7870546329718815No Hit
TAGCAGCACGTAAATATTGGCG2680783.0711656998364507No Hit
AACATTCAACGCTGTCGGTGAG2577052.952330130321595No Hit
AAGCTGCCAGTTGAAGAACTGT2506362.871345975216947No Hit
TATTGCACTTGTCCCGGCCTGTAA1783042.0426932793576444No Hit
TATTGCACTTGTCCCGGCCTGTAT1652911.8936132382801527No Hit
CCCCCACTGCTAAATTTGACTG1177001.3483993571675044No Hit
TGAGGTAGTAGGTTGTATAGTT1161431.3305619926890864No Hit
TATTGCACTTGTCCCGGCCTGT1153041.320950207976567No Hit
TAGCTTATCAGACTGATGTTGACT1102441.2629816374815153No Hit
TAGCTTATCAGACTGATGTTGAC1069811.2255999288796668No Hit
TAGCTTATCAGACTGATGTTGA796640.9126498418809862No Hit
TATTGCACTTGTCCCGGCCTGTA699890.8018107273474635No Hit
TGTAAACATCCTTGACTGGAAGCT625400.7164732013360724No Hit
TGAGGTAGTAGATTGTATAGTT583240.6681736967496816No Hit
CATAAAGTAGAAAGCACTACT576760.660750053729762No Hit
AGCAGCATTGTACAGGGCTATGA567690.6503592447497202No Hit
TTCAAGTAATTCAGGATAGGTT522720.5988405369401851No Hit
TTAATGCTAATCGTGATAGGGGTT491660.5632574578971752No Hit
CAACGGAATCCCAAAAGCAGCTGT481980.5521678183242088No Hit
TGTAAACATCCCCGACTGGAAGCT451120.5168138640657644No Hit
ACTGGACTTGGAGTCAGAAGGC443290.5078436287500281No Hit
CACCCGGCTGTGTGCACATGTGC406500.46569612462921883No Hit
CAACGGAATCCCAAAAGCAGCT403630.462408183970705No Hit
CCCATAAAGTAGAAAGCACTAC384490.4404809420877942No Hit
CATTGCACTTGTCTCGGTCTGA368390.42203639693027784No Hit
CGCCTGTCTGAGCGTCGCT356190.40805978507178714No Hit
GCCTGTCTGAGCGTCGCT336830.38588050592585427No Hit
TCGTACGACTCTTAGCGG306840.3515232444802694No Hit
ATCCCGGACGAGCCCCCA303860.3481092851902446No Hit
CTACGCCTGTCTGAGCGTCGCT293160.3358511092159946No Hit
CCCATAAAGTAGAAAGCACT284230.3256206875851485No Hit
TGAGGTAGTAGTTTGTACAGTT282310.32342108965332045No Hit
TACGCCTGTCTGAGCGTCGCT275090.3151496849305087No Hit
AACATTCAACGCTGTCGGTGAGTT242180.27744720163026865No Hit
TGATGTAGTAGGTTGTATAGTT226280.25923178125731766No Hit
TGTAAACATCCTACACTCTCAGCT218450.2502615459415814No Hit
AGCTCGGTCTGAGGCCCCTCAGT217520.2491961156933522No Hit
TTCTCACTACTGCACTTGACTAG216640.24818796664126436No Hit
TATTGCACTTGTCCCGGCCTGTT207700.23794608877119006No Hit
TATTGCACTTGTCCCGGCCTGTAG207110.23727017065672207No Hit
CTCCCACTGCTTCACTTGACTAG205570.2355059098155683No Hit
AACGGAATCCCAAAAGCAGCTG192480.22050969266576145No Hit
ACCGGGTGCTGTAGGCTTA192360.22037221779502222No Hit
CACTAGATTGTGAGCTCCTGGA189700.2173248581603021No Hit
CAACACCAGTCGATGGGCTGT184940.21187168828764508No Hit
ACCGGGTGCTGTAGGCTT177190.20299310288573502No Hit
TGAGAACTGAATTCCATGGGTT176230.201893303919821No Hit
CAAAGAATTCTCCTTTTGGGCT174370.19976244342336255No Hit
CACTAGATTGTGAGCTCCTGGAA167330.19169725100665974No Hit
TCGTTTCCCGGCCAATGCACCA166450.1906891019545719No Hit
TGAGGTAGTAGTTTGTGCTGTT164610.18858115393657002No Hit
TGAGGTAGTAGGTTGTGTGGTT162340.1859805876317525No Hit
TAGCAGCACGTAAATATTGGC160190.18351749619767418No Hit
CCCCCACTGCTAAATTTGACTGGCTT159880.1831623527815978No Hit
TTCACAGTGGCTAAGTTCTGC154850.17739986444977746No Hit
TTCAAGTAATCCAGGATAGGC154320.17679268377067908No Hit
TAGCTTATCAGACTGATGTTGACA152880.17514298532180805No Hit
CTTCTCACTACTGCACTTGACTAG152740.17498259797261226No Hit
TATTGCACTTGTCCCGGCCTGTTT147270.16871603511474798No Hit
CAACGGAATCCCAAAAGCAGCTGA145000.16611546880993044No Hit
TTCAAGTAATCCAGGATAGGCTT143120.1639616958350155No Hit
TTCACAGTGGCTAAGTTCCGC140510.16097161739643673No Hit
ACCGGGTGCTGTAGGCTTT140110.16051336782730588No Hit
CGCGACCTCAGATCAGACGTGGCG134880.1545217547109201No Hit
CGCGACCTCAGATCAGACGTGG126660.14510472606528133No Hit
TCCTGTCTGAGCGTCGCT125300.14354667753023645No Hit
ACTGGACTTGGAGTCAGAAGGCA123310.1412668859238105No Hit
AGCAGCATTGTACAGGGCTATGT122990.14090028626850581No Hit
TGTAAACATCCCCGACTGGAAGC122470.14030456182863574No Hit
TTCACAGTGGCTAAGTTCCG121750.13947971260420022No Hit
TATTGCACTTGTCCCGGCCTGA120580.13813933261449252No Hit
AACATTCAACGCTGTCGGTGAGA119990.1374634145000245No Hit
ACTGGACTTGGAGTCAGAAGGCT119700.13713118356240467No Hit
CGTTCGCGCTTTCCCCTG118800.13610012203186028No Hit
TATTGCACTTGTCCCGGCCTGTAAT115180.13195296343122614No Hit
TAGCAGCACATCATGGTTTACA115100.13186131351739996No Hit
ATCCCACCGCTGCCACCA113830.13040637113540954No Hit
TGATGTAGTAGATTGTATAGTT112570.1289628849926474No Hit
TGAGGTAGTAGGTTGTATAGT110340.12640814364474295No Hit
CAAAGAATTCTCCTTTTGGGCTT107550.12321185290005532No Hit
CCCCCCACTGCTAAATTTGACTGGCT103930.11906469429942118No Hit
ACCACAGGGTAGAACCACGGAC103150.11817110763961605No Hit
TATTGCACTTGTCCCGGCCTGTAAA100450.11507792304798285No Hit
ACCCCCCACTGCTAAATTTGACTG99330.1137948242544165No Hit
TTCACAGTGGCTAAGTTCTG98730.11310744990072022No Hit
GTCAGGATGGCCGAGCGGTCTAAGG97850.11209930084863239No Hit
AGAGGTAGTAGGTTGCATAGTT95100.10894883506085784No Hit
TCGTTTCCCGGGCGGCGCACCA94910.10873116651552069No Hit
AACATTCAACGCTGTCGGTGA94770.10857077916632488No Hit
AACCGGGCGGAAACACCA93510.10712729302356273No Hit
TGTCAGTTTGTCAAATACCCCAA93160.10672632465057325No Hit
AATGCACCCGGGCAAGGATTCT92690.10618788140684451No Hit
CCCCCCACTGCTAAATTTGACTGG92440.10590147542613773No Hit
ATCACATTGCCAGGGATTTCC91030.10428614569495151No Hit
CAAAGTGCTGTTCGTGCAGGTAG90570.10375915869045105No Hit
ACTTGACTTGGAGTCAGAAGGC89850.10293430946601552No Hit
TGTAAACATCCTACACTCAGCT88760.10168557939013398No Hit
CAAAGTGCTGTTCGTGCAGGT88080.10090655512261155No Hit
ACTGGACTTGGAGTCAGAAGG87980.10079199273032884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)