FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11181991
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7908877.072863857608184No Hit
CCCCCCACTGCTAAATTTGACTG6278745.615046551191107No Hit
AACATTCAACGCTGTCGGTGAGT4950314.427038082931743No Hit
TTCAAGTAATCCAGGATAGGCT4477354.00407226226528No Hit
GGAATACCGGGTGCTGTAGGCTTT3983683.562585589632473No Hit
AAGCTGCCAGTTGAAGAACTGT3354603.000002414596828No Hit
TAGCAGCACGTAAATATTGGCG3292632.9445829459172344No Hit
AACATTCAACGCTGTCGGTGAG2717802.430515281223174No Hit
GGAATACCGGGTGCTGTAGGCTT2578022.305510709139365No Hit
CCCCCACTGCTAAATTTGACTG2047961.8314806370350327No Hit
TATTGCACTTGTCCCGGCCTGTAA2039151.8236018970145835No Hit
TATTGCACTTGTCCCGGCCTGTAT1792621.6031313207102387No Hit
TGAGGTAGTAGGTTGTATAGTT1551411.387418394452294No Hit
TAGCTTATCAGACTGATGTTGACT1372001.2269729067032875No Hit
TAGCTTATCAGACTGATGTTGAC1113870.9961285069894977No Hit
TGAATACCGGGTGCTGTAGGCTTT1080980.9667151404432359No Hit
TATTGCACTTGTCCCGGCCTGT1036020.9265076317804226No Hit
TGAGGTAGTAGATTGTATAGTT825850.7385536260939577No Hit
TAGCTTATCAGACTGATGTTGA791980.7082638503286222No Hit
TGTAAACATCCTTGACTGGAAGCT784640.7016997241367838No Hit
TTAATGCTAATCGTGATAGGGGTT781660.6990347246747024No Hit
TGAATACCGGGTGCTGTAGGCTT699940.6259529273454074No Hit
CATAAAGTAGAAAGCACTACT680520.6085857160858026No Hit
GGAATACCGGGTGCTGTAGGCTTA665200.5948851148243636No Hit
TGTAAACATCCCCGACTGGAAGCT655910.5865771131455927No Hit
TATTGCACTTGTCCCGGCCTGTA638280.5708106901534798No Hit
CAACGGAATCCCAAAAGCAGCTGT580870.5194692072279435No Hit
CCCATAAAGTAGAAAGCACTAC565270.5055182033324834No Hit
ACTGGACTTGGAGTCAGAAGGC528460.47259919990992655No Hit
AGCAGCATTGTACAGGGCTATGA511200.4571636661127701No Hit
CGCCTGTCTGAGCGTCGCT471430.4215975491305618No Hit
TTCAAGTAATTCAGGATAGGTT446770.3995442314342768No Hit
CAACGGAATCCCAAAAGCAGCT445490.39839953367875186No Hit
TGAGGTAGTAGTTTGTACAGTT377530.33762323722134996No Hit
CACCCGGCTGTGTGCACATGTGC371810.3325078691263479No Hit
CCCATAAAGTAGAAAGCACT367440.32859979944537604No Hit
TGTAAACATCCTACACTCTCAGCT362220.32393157891112595No Hit
GGAATACCGGGTGCTGTAGGCT361830.32358280381373944No Hit
CGCGACCTCAGATCAGAC322290.28822237470947704No Hit
TCGTTTCCCGGCCAATGCACCA309560.276837997812733No Hit
AACATTCAACGCTGTCGGTGAGTT304060.2719193746444618No Hit
TTCTCACTACTGCACTTGACTAG297530.26607962750104164No Hit
CAAAGAATTCTCCTTTTGGGCT295990.2647024130139257No Hit
CATTGCACTTGTCTCGGTCTGA279930.2503400333625738No Hit
TCGTACGACTCTTAGCGG270430.2418442297082872No Hit
TGAGAACTGAATTCCATGGGTT267010.23878574039274403No Hit
ATCCCGGACGAGCCCCCA266120.2379898177346056No Hit
CCCCCACTGCTAAATTTGACTGGCTT260900.23332159720035545No Hit
CTCCCACTGCTTCACTTGACTAG238750.2135129602590451No Hit
ACCGGGTGCTGTAGGCTTA236280.2113040513089306No Hit
TATTGCACTTGTCCCGGCCTGTAG229410.2051602438241991No Hit
CTTCTCACTACTGCACTTGACTAG225480.20164566399668896No Hit
CTACGCCTGTCTGAGCGTCGCT216300.19343603478128357No Hit
TCCACCACGTTCCCGTGG212050.1896352805148922No Hit
ACCGGGTGCTGTAGGCTT202310.18092484603144468No Hit
TTCAAGTAATCCAGGATAGGC200940.1796996617149844No Hit
AACGGAATCCCAAAAGCAGCTG200480.17928828595909263No Hit
CCCCCCACTGCTAAATTTGACTGGCT194550.17398511588857477No Hit
CAAAGAATTCTCCTTTTGGGCTT194310.17377048505941384No Hit
TGAGGTAGTAGTTTGTGCTGTT194260.17372577030333863No Hit
TGAATACCGGGTGCTGTAGGCTTA193660.17318919323043636No Hit
CACTAGATTGTGAGCTCCTGGA189720.16966567045171116No Hit
CACTAGATTGTGAGCTCCTGGAA189100.16911120747637876No Hit
CAACACCAGTCGATGGGCTGT186740.16700067098962967No Hit
TTCACAGTGGCTAAGTTCTGC185360.16576654372195435No Hit
TGTAAACATCCCCGACTGGAAGC184380.1648901345028806No Hit
TATTGCACTTGTCCCGGCCTGTT183120.16376332264978571No Hit
AACATTCAACGCTGTCGGTGA181490.16230562160173442No Hit
TGAGGTAGTAGGTTGTGTGGTT178100.15927396113983638No Hit
TTCAAGTAATCCAGGATAGGCTT178030.1592113604813311No Hit
AGCTCGGTCTGAGGCCCCTCAGT176910.15820974994524678No Hit
ACCGGGTGCTGTAGGCTTT172070.15388136155716814No Hit
TAGCAGCACGTAAATATTGGC170100.15211960016780554No Hit
TGTCAGTTTGTCAAATACCCCAA167140.14947248660815413No Hit
TACGCCTGTCTGAGCGTCGCT162550.14536767200045145No Hit
ACTGGACTTGGAGTCAGAAGGCT158720.14194252168509167No Hit
TAGCAGCACATCATGGTTTACA156960.1403685622712449No Hit
TATTGCACTTGTCCCGGCCTGTTT154270.13796290839439954No Hit
CCCCCCACTGCTAAATTTGACTGG153980.13770356280916343No Hit
TGTAAACATCCTACACTCAGCT153870.137605190345798No Hit
TGAGAACTGAATTCCATAGGCTGT151290.13529790893231805No Hit
TATTGCACTTGTCCCGGCCTGTAAT151290.13529790893231805No Hit
TAGCTTATCAGACTGATGTTGACA148000.13235567798257036No Hit
TGAGGTAGTAGGTTGTATAGT143280.12813460500907217No Hit
TTCACAGTGGCTAAGTTCCG139890.1251029445471741No Hit
ACCCCCCACTGCTAAATTTGACTG138920.12423547827931539No Hit
ACTGGACTTGGAGTCAGAAGGCA137230.12272411952397387No Hit
TTCACAGTGGCTAAGTTCCGC136410.1219907975243407No Hit
AACATTCAACGCTGTCGGTGAGA136170.12177616669517978No Hit
ACGCCTGTCTGAGCGTCGCT135130.12084609976881577No Hit
TATTGCACTTGTCCCGGCCTGTAAA131710.11778761045327257No Hit
CAACGGAATCCCAAAAGCAGCTGA131530.11762663733140191No Hit
CACCCGTAGAACCGACCTTGCG127090.11365596699192478No Hit
GCCTGTCTGAGCGTCGCT123290.11025764553021014No Hit
TGATGTAGTAGGTTGTATAGTT123200.11017715896927478No Hit
AATGCACCCGGGCAAGGATTCT122040.10913977662833031No Hit
AGAGGTAGTAGGTTGCATAGTT119650.10700241128793611No Hit
TTCACAGTGGCTAAGTTCTG118910.10634063289802326No Hit
ATCACATTGCCAGGGATTTCC116380.10407806624061851No Hit
GTCAGGATGGCCGAGCGGTCTAAGG115070.10290653963144845No Hit
GGAATACCGGGTGCTGTAGGCTA114740.1026114222413522No Hit
CAAAGTGCTGTTCGTGCAGGT114070.1020122445099446No Hit
AACCGGGCGGAAACACCA112450.10056348641310836No Hit
ACCACAGGGTAGAACCACGGAC112210.10034885558394743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)