FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6358557
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4258016.696503624957676No Hit
TTCAAGTAATCCAGGATAGGCT2909644.5759438816071No Hit
CCCCCCACTGCTAAATTTGACTG2724614.284950186024911No Hit
AACATTCAACGCTGTCGGTGAGT2646544.162170756666961No Hit
AAGCTGCCAGTTGAAGAACTGT2217373.4872220222292576No Hit
TAGCAGCACGTAAATATTGGCG1992333.1333052451995003No Hit
AACATTCAACGCTGTCGGTGAG1782822.803812248596655No Hit
CCCCCACTGCTAAATTTGACTG1092401.717999854369474No Hit
TGAGGTAGTAGGTTGTATAGTT1082451.7023516499105065No Hit
TATTGCACTTGTCCCGGCCTGTAA749991.1794971720785077No Hit
TATTGCACTTGTCCCGGCCTGTAT650721.0233768447778324No Hit
TATTGCACTTGTCCCGGCCTGT644391.0134217559109715No Hit
CATAAAGTAGAAAGCACTACT633130.9957133355885619No Hit
TAGCTTATCAGACTGATGTTGACT564190.8872925099200967No Hit
TAGCTTATCAGACTGATGTTGAC560050.880781598718074No Hit
TGAGGTAGTAGATTGTATAGTT542340.8529293674649767No Hit
TAGCTTATCAGACTGATGTTGA519750.8174024389495919No Hit
TTAATGCTAATCGTGATAGGGGTT509360.8010622535899261No Hit
ACCGGGTGCTGTAGGCTT432030.6794466102922408No Hit
ACCGGGTGCTGTAGGCTTA398840.6272492328054934No Hit
CGCCTGTCTGAGCGTCGCT380400.5982489423307835No Hit
TGTAAACATCCTTGACTGGAAGCT378370.595056394084381No Hit
TATTGCACTTGTCCCGGCCTGTA359890.5659931962550623No Hit
TTCAAGTAATTCAGGATAGGTT358930.5644834197444483No Hit
ACCGGGTGCTGTAGGCTTT343150.5396664683512312No Hit
CCCATAAAGTAGAAAGCACTAC269670.42410565793465405No Hit
CAACGGAATCCCAAAAGCAGCTGT260850.41023458624338827No Hit
AGCAGCATTGTACAGGGCTATGA255980.4025756158197528No Hit
TGAGGTAGTAGTTTGTACAGTT253320.39839227673825994No Hit
TCGTACGACTCTTAGCGG252610.3972756711939517No Hit
TGTAAACATCCCCGACTGGAAGCT249440.3922902633411952No Hit
CCCATAAAGTAGAAAGCACT244560.3846155660789075No Hit
TTCTCACTACTGCACTTGACTAG243160.3824138086675955No Hit
ACTGGACTTGGAGTCAGAAGGC239700.3769723224939243No Hit
CATTGCACTTGTCTCGGTCTGA193810.30480185991884634No Hit
CTACGCCTGTCTGAGCGTCGCT190180.2990930174880873No Hit
ATCCCGGACGAGCCCCCA182090.28637000501843424No Hit
TTCACAGTGGCTAAGTTCCG177490.279135659238409No Hit
CAACACCAGTCGATGGGCTGT172530.27133514726690344No Hit
AACATTCAACGCTGTCGGTGAGTT169480.26653846147797366No Hit
TGAGAACTGAATTCCATGGGTT157810.2481852407708227No Hit
CACCCGGCTGTGTGCACATGTGC153640.24162714905284327No Hit
TGTAAACATCCTACACTCTCAGCT146330.23013082999806403No Hit
CTCCCACTGCTTCACTTGACTAG145820.22932876122680035No Hit
TTCAAGTAATCCAGGATAGGC142190.22361991879604132No Hit
TGAGGTAGTAGTTTGTGCTGTT135930.21377491779974608No Hit
TACGCCTGTCTGAGCGTCGCT133830.210472281682778No Hit
TGAGGTAGTAGGTTGTGTGGTT131280.2064619378264597No Hit
CTTCTCACTACTGCACTTGACTAG128510.2021056035197923No Hit
ACGCCTGTCTGAGCGTCGCT127320.20023410972017708No Hit
CAAAGAATTCTCCTTTTGGGCT124300.19548460444720397No Hit
AGCTCGGTCTGAGGCCCCTCAGT123050.1935187496156754No Hit
TGATGTAGTAGGTTGTATAGTT119160.18740100937995838No Hit
AACGGAATCCCAAAAGCAGCTG117520.18482180784099286No Hit
TAGCAGCACGTAAATATTGGC115930.18232124049528847No Hit
TGAGGTAGTAGGTTGTATAGT115860.18221115262472287No Hit
TTCACAGTGGCTAAGTTCTG113690.17879842863718923No Hit
TATTGCACTTGTCCCGGCCTGTT107060.16837153461076154No Hit
CCCCCACTGCTAAATTTGACTGGCTT106660.1677424610646724No Hit
AACATTCAACGCTGTCGGTGA94790.14907470358447678No Hit
TTCACAGTGGCTAAGTTCTGC93580.1471717561075571No Hit
GCCTGTCTGAGCGTCGCT91580.14402638837711135No Hit
TTCAAGTAATCCAGGATAGGCTT91410.14375903212002347No Hit
TGTAAACATCCTACACTCAGCT89370.14055075703496878No Hit
GGAATACCGGGTGCTGTAGGCTTT85700.13477900724960082No Hit
TATTGCACTTGTCCCGGCCTGTAG85280.13411848002620722No Hit
TTCACAGTGGCTAAGTTCCGC84870.1334736796414658No Hit
CAAAGTGCTGTTCGTGCAGGT84430.13278169874076776No Hit
CCCCCCACTGCTAAATTTGACTGG81900.12880280856175388No Hit
AGAGGTAGTAGGTTGCATAGTT81300.12785919824262015No Hit
CCCCCCACTGCTAAATTTGACTGGCT79680.12531145038095906No Hit
TAGCAGCACATCATGGTTTACA77770.12230762419838338No Hit
CACTAGATTGTGAGCTCCTGGA77720.12222899000512222No Hit
TGTCAGTTTGTCAAATACCCCAA77710.12221326316647001No Hit
CAACGGAATCCCAAAAGCAGCT76020.11955542743424333No Hit
GGAATACCGGGTGCTGTAGGCTT74820.11766820679597588No Hit
TTATAAAGCAATGAGACTGATT70700.11118874927125763No Hit
TGAGGTAGTAAGTTGTATTGTT70500.11087421249821304No Hit
ATCCCACCGCTGCCACCA70500.11087421249821304No Hit
TGTAAACATCCCCGACTGGAAGC70110.11026086579077612No Hit
ACTGGACTTGGAGTCAGAAGGCT69900.10993060217907931No Hit
ACCACAGGGTAGAACCACGGAC69580.109427343342208No Hit
CAAAGAATTCTCCTTTTGGGCTT68600.10788611315428957No Hit
ACTGGACTTGGAGTCAGAAGG67480.10612470722523994No Hit
AACATTCAACGCTGTCGGTGAGA67000.10536981896993297No Hit
AGCTACATTGTCTGCTGGGTTT66420.10445766232810368No Hit
ACCCCCCACTGCTAAATTTGACTG66370.10437902813484254No Hit
CACTAGATTGTGAGCTCCTGGAA65810.10349832517031773No Hit
TATTGCACTTGTCCCGGCCTG65470.10296361265614196No Hit
AACCGGGCGGAAACACCA65130.10242890014196618No Hit
CCCATAAAGTAGAAAGCACTA64850.10198854865970378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)