FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637505
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5182517.80791878876174No Hit
CCCCCCACTGCTAAATTTGACTG3873885.836349652467305No Hit
TAGCAGCACGTAAATATTGGCG2936594.424237721854824No Hit
AACATTCAACGCTGTCGGTGAGT2852584.297669078968679No Hit
TTCAAGTAATCCAGGATAGGCT2427293.6569313318784693No Hit
AAGCTGCCAGTTGAAGAACTGT2218013.3416321343637407No Hit
AACATTCAACGCTGTCGGTGAG1797862.7086382609127977No Hit
CCCCCACTGCTAAATTTGACTG1555042.342808028016551No Hit
CGCCTGTCTGAGCGTCGCT1189381.7919082546830474No Hit
TAGCTTATCAGACTGATGTTGA1013911.5275468719044278No Hit
TGAGGTAGTAGGTTGTATAGTT994171.497806781313159No Hit
TAGCTTATCAGACTGATGTTGAC974831.468669326802767No Hit
TAGCTTATCAGACTGATGTTGACT923061.3906731520352904No Hit
CATAAAGTAGAAAGCACTACT714901.0770613355470164No Hit
TATTGCACTTGTCCCGGCCTGTAA676411.019072678664649No Hit
TATTGCACTTGTCCCGGCCTGT646080.9733777978321675No Hit
TATTGCACTTGTCCCGGCCTGTAT530350.7990201137324944No Hit
TGTAAACATCCTTGACTGGAAGCT495520.7465455769901491No Hit
TGAGGTAGTAGATTGTATAGTT463090.6976868567330646No Hit
GCCTGTCTGAGCGTCGCT403420.6077886193682717No Hit
AGCAGCATTGTACAGGGCTATGA400830.6038865507445945No Hit
TTCAAGTAATTCAGGATAGGTT388020.5845871302545158No Hit
ACCGGGTGCTGTAGGCTT333400.5022971734107922No Hit
CATTGCACTTGTCTCGGTCTGA325960.4910881423064842No Hit
ACTGGACTTGGAGTCAGAAGGC320410.4827265666843189No Hit
TATTGCACTTGTCCCGGCCTGTA312460.47074917457689297No Hit
TGTAAACATCCCCGACTGGAAGCT305570.46036876808379057No Hit
CAACACCAGTCGATGGGCTGT297450.44813525564199197No Hit
CCCATAAAGTAGAAAGCACT288100.4340486372514974No Hit
TGAGGTAGTAGTTTGTACAGTT250510.3774159115511024No Hit
TCGTACGACTCTTAGCGG246580.3714950120564881No Hit
CAACGGAATCCCAAAAGCAGCTGT239490.3608132875229473No Hit
CACCCGGCTGTGTGCACATGTGC238690.3596080153611937No Hit
CCCATAAAGTAGAAAGCACTAC233450.35171348270170794No Hit
TACGCCTGTCTGAGCGTCGCT221920.3343424976704349No Hit
ACGCCTGTCTGAGCGTCGCT219390.33053082445888926No Hit
CTACGCCTGTCTGAGCGTCGCT205780.310026131807057No Hit
ACCGGGTGCTGTAGGCTTT199960.3012577768303No Hit
CAACGGAATCCCAAAAGCAGCT196980.29676813802776797No Hit
TTCACAGTGGCTAAGTTCCG186380.28079828188453343No Hit
ACCGGGTGCTGTAGGCTTA183080.2758265342173No Hit
AACATTCAACGCTGTCGGTGAGTT177950.26809772648005536No Hit
GCGTTCGCGCTTTCCCCTG168350.2536344605390128No Hit
TTCTCACTACTGCACTTGACTAG162620.24500169868045296No Hit
TTCACAGTGGCTAAGTTCTG152000.2290017107331746No Hit
TGAGGTAGTAGTTTGTGCTGTT142870.2152465421871622No Hit
AACGGAATCCCAAAAGCAGCTG141090.21256481162726057No Hit
TGTAAACATCCTACACTCTCAGCT139500.21016933320577535No Hit
ATCCCGGACGAGCCCCCA136080.20501679471427894No Hit
TAGCAGCACGTAAATATTGGC127060.19142735109050765No Hit
CACTAGATTGTGAGCTCCTGGA126250.19020701302673218No Hit
TTCAAGTAATCCAGGATAGGC125470.18903187266902247No Hit
TCGTTTCCCGGCCAATGCACCA119230.17963074980734478No Hit
TTCACAGTGGCTAAGTTCTGC113620.171178778773048No Hit
TTCAAGTAATCCAGGATAGGCTT113230.17059120859419316No Hit
TTATAAAGCAATGAGACTGATT112780.1699132430032068No Hit
CTTCTCACTACTGCACTTGACTAG111720.1683162573888833No Hit
TAGCTTATCAGACTGATGTTGACA108720.16379648678230752No Hit
CAAAGAATTCTCCTTTTGGGCT102950.15510346131566002No Hit
ACCACAGGGTAGAACCACGGAC102720.1547569455691559No Hit
TCCTGTCTGAGCGTCGCT100570.1515177766344432No Hit
TATTGCACTTGTCCCGGCCTGTT98710.1487155188583662No Hit
CTCCCACTGCTTCACTTGACTAG98230.14799235556131407No Hit
TGATGTAGTAGGTTGTATAGTT97390.14672681979147284No Hit
TGAGGTAGTAGGTTGTATAGT97050.1462145791227276No Hit
TGAGGTAGTAGGTTGTGTGGTT96210.14494904335288636No Hit
TTCACAGTGGCTAAGTTCCGC94670.14262889444151078No Hit
CACTAGATTGTGAGCTCCTGGAA92080.13872682581783366No Hit
CCCCCACTGCTAAATTTGACTGGCTT91760.13824471695313223No Hit
AGCTCGGTCTGAGGCCCCTCAGT91630.1380488602268473No Hit
ACTGGACTTGGAGTCAGAAGGCA89300.13453850505574005No Hit
ACTGGACTTGGAGTCAGAAGGCT89040.13414679160317017No Hit
TGTAAACATCCCCGACTGGAAGC88750.1337098804445345No Hit
AGCAGCATTGTACAGGGCTATG84520.1273370038892626No Hit
TAGCAGCACGTAAATATTGGCGT84410.1271712789670215No Hit
AGCAGCATTGTACAGGGCTATGT84160.1267946314164735No Hit
CAAAGTGCTGTTCGTGCAGGT83530.1258454795890926No Hit
AACATTCAACGCTGTCGGTGA83400.12564962286280762No Hit
TATTGCACTTGTCCCGGCCTGTAG75130.11319012189068031No Hit
ACTGGACTTGGAGTCAGAAGG74840.11275321073204464No Hit
AGAATTACTCTGAGACCT74660.11248202449565009No Hit
AATGACACGATCACTCCCGTTG73220.1103125346044937No Hit
CAAAGAATTCTCCTTTTGGGCTT73050.11005641427012107No Hit
AGAGGTAGTAGGTTGCATAGTT71770.1081279788113154No Hit
TAGTGGGTTATCAGAACT70500.10621460925453163No Hit
AACATTCAACGCTGTCGGTGAGA69740.10506960070086575No Hit
CCCCCCACTGCTAAATTTGACTGG69730.10505453479884383No Hit
ATCCCACCGCTGCCACCA69120.10413551477550675No Hit
CCCCCCACTGCTAAATTTGACTGGCT69040.1040149875593314No Hit
TGAGGTAGTAAGTTGTATTGTT68700.10350274689058614No Hit
CCAAGTCCTTCTGATCGAGGCCC67490.10167977274593389No Hit
TTAATGCTAATCGTGATAGGGGTT67340.10145378421560511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)