FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9519787
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105171211.047642137371351No Hit
CAACGGAATCCCAAAAGCAGCTG6640936.975922885669606No Hit
CCCCCACTGCTAAATTTGACTG3151643.3106202901388446No Hit
AACATTCAACGCTGTCGGTGAGT2517042.6440087367500977No Hit
TAGCAGCACGTAAATATTGGCG2443222.5664649849833827No Hit
TTCAAGTAATCCAGGATAGGCT2317432.4343296756534576No Hit
AAGCTGCCAGTTGAAGAACTGT2044782.1479262088532023No Hit
AACATTCAACGCTGTCGGTGAG1672161.7565098882989714No Hit
TAGCTTATCAGACTGATGTTGACT1512571.5888695829013821No Hit
TATTGCACTTGTCCCGGCCTGTAA1427681.4996974197006718No Hit
TAGCTTATCAGACTGATGTTGAC1408001.479024688262458No Hit
TATTGCACTTGTCCCGGCCTGTAT1153481.2116657652109233No Hit
TAGCTTATCAGACTGATGTTGA928870.9757256123482595No Hit
TGAGGTAGTAGGTTGTATAGTT860290.9036861854157032No Hit
TGTAAACATCCTTGACTGGAAGCT785750.8253861142061267No Hit
TATTGCACTTGTCCCGGCCTGT705410.7409934697068328No Hit
GGAATACCGGGTGCTGTAGGCTTT570350.599120547550066No Hit
TCGTACGACTCTTAGCGG556800.5848870358128812No Hit
TTCAAGTAATTCAGGATAGGTT522800.5491719510110888No Hit
TATTGCACTTGTCCCGGCCTGTA522480.5488358090364837No Hit
TGAGGTAGTAGATTGTATAGTT515100.5410835347471535No Hit
CATAAAGTAGAAAGCACTACT508410.5340560665905656No Hit
AGCAGCATTGTACAGGGCTATGA495240.5202217234482243No Hit
TGTAAACATCCCCGACTGGAAGCT470730.49447534908081453No Hit
CAACGGAATCCCAAAAGCAGCTGT419200.4403459867326863No Hit
GGAATACCGGGTGCTGTAGGCTT402790.42310820609746835No Hit
ACCGGGTGCTGTAGGCTTA399110.4192425733895096No Hit
ACTGGACTTGGAGTCAGAAGGC390670.41037682880930004No Hit
CCCATAAAGTAGAAAGCACTAC335290.35220325832920424No Hit
ACCGGGTGCTGTAGGCTT334730.35161500987364525No Hit
CCCCCACTGCTAAATTTGACTGGCTT333500.3503229641587569No Hit
TTCTCACTACTGCACTTGACTAG332820.3496086624627211No Hit
ACCGGGTGCTGTAGGCTTT308410.32396733246237547No Hit
CACCCGGCTGTGTGCACATGTGC296400.3113515039779777No Hit
CAACGGAATCCCAAAAGCAGCT269350.28293700268713995No Hit
CATTGCACTTGTCTCGGTCTGA265910.2793234764601351No Hit
CTTCTCACTACTGCACTTGACTAG261490.2746805154359021No Hit
ACCCCCCACTGCTAAATTTGACTG242230.25444897033935737No Hit
CTCCCACTGCTTCACTTGACTAG230900.2425474435509954No Hit
TGAATACCGGGTGCTGTAGGCTTT229280.24084572480455707No Hit
TAGCTTATCAGACTGATGTTGACA226800.23824062450136752No Hit
GCCTGTCTGAGCGTCGCT218140.2291437823136169No Hit
CGCGACCTCAGATCAGACGTGGCG214600.22542521171954794No Hit
CAAAGAATTCTCCTTTTGGGCT214380.2251941141120069No Hit
TGAGGTAGTAGTTTGTACAGTT211820.22250497831516608No Hit
CGCGACCTCAGATCAGACGTGG206320.21672753812664083No Hit
AACATTCAACGCTGTCGGTGAGTT197840.20781977579960562No Hit
CAACACCAGTCGATGGGCTGT191880.20155913152258556No Hit
TCGTTTCCCGGCCAATGCACCA181510.1906660306580389No Hit
CCCATAAAGTAGAAAGCACT180220.18931095832291206No Hit
CTCCCACTGCTTCACTTGACTAGCCT178990.1880189126080237No Hit
TAGCAGCACGTAAATATTGGC178280.1872730976018686No Hit
TGTAAACATCCTACACTCTCAGCT175880.18475203279233035No Hit
CCCCCCACTGCTAAATTTGACTGGCT175800.18466799729867905No Hit
TGATGTAGTAGGTTGTATAGTT170860.17947880556571277No Hit
TGTCAGTTTGTCAAATACCCCAA170040.1786174417557872No Hit
CCCCCCACTGCTAAATTTGACTGG168480.1769787496295873No Hit
TGAATACCGGGTGCTGTAGGCTT162620.17082314971963133No Hit
CACTAGATTGTGAGCTCCTGGA157300.16523478939182148No Hit
CACTAGATTGTGAGCTCCTGGAA155970.163837699309869No Hit
CTACGCCTGTCTGAGCGTCGCT154870.16268221127216398No Hit
TATTGCACTTGTCCCGGCCTGTAG149590.15713586869117976No Hit
CAAAGAATTCTCCTTTTGGGCTT144750.15205172132527756No Hit
TACGCCTGTCTGAGCGTCGCT138590.1455809883141293No Hit
AACGGAATCCCAAAAGCAGCTG135610.14245066617561927No Hit
TATTGCACTTGTCCCGGCCTGTT132590.13927832629028358No Hit
TGAGGTAGTAGTTTGTGCTGTT126660.13304919532338277No Hit
TGTCAGTTTGTCAAATACCCCA123050.12925709367236893No Hit
TATTGCACTTGTCCCGGCCTGTAAT120470.12654694900211527No Hit
TGTAAACATCCCCGACTGGAAGC116460.12233466988284508No Hit
CGCCTGTCTGAGCGTCGCT113730.11946695866199526No Hit
ACTGGACTTGGAGTCAGAAGGCA112870.11856357710524407No Hit
ATGCACCTGGGCAAGGATTCTGA110600.1161790699728891No Hit
TATTGCACTTGTCCCGGCCTGTAAA110450.11602150342229296No Hit
TTCAAGTAATCCAGGATAGGCTT110020.11556981264391736No Hit
ACTGGACTTGGAGTCAGAAGGCT109890.11543325496673401No Hit
TACCACCCTGAACGCGCCCGA109200.11470844883399176No Hit
TGAGAACTGAATTCCATGGGTT107560.11298572121414062No Hit
TTATAAAGCAATGAGACTGATT102460.10762845849387177No Hit
AGCAGCATTGTACAGGGCTATGT102320.10748139637998202No Hit
AACCGGGCGGAAACACCA102160.10731332539267947No Hit
TGATGTAGTAGATTGTATAGTT101300.10640994383592826No Hit
CGCGACCTCAGATCAGACGTGGC100740.10582169538036933No Hit
ACCACCCTGAACGCGCCCGA100330.10539101347540655No Hit
CCCCCCACTGCTAAATTTGACTGTA99500.10451914522877455No Hit
GGAATACCGGGTGCTGTAGGCTTA99090.10408846332381178No Hit
GGGAATACCGGGTGCTGTAGGCTTT99060.10405695001369253No Hit
TTCAAGTAATCCAGGATAGGC98770.10375232134920666No Hit
GGGAATACCGGGTGCTGTAGGCTT98070.10301701077975799No Hit
TTCACAGTGGCTAAGTTCCGC97610.10253380669126315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)