FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7270885
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG92618612.73828426663329No Hit
CAACGGAATCCCAAAAGCAGCTG4725566.499291351740538No Hit
CCCCCACTGCTAAATTTGACTG2782893.8274432892282024No Hit
AACATTCAACGCTGTCGGTGAGT2289523.1488876526035No Hit
TTCAAGTAATCCAGGATAGGCT1753322.4114258443091865No Hit
TAGCAGCACGTAAATATTGGCG1689032.3230046961270876No Hit
AAGCTGCCAGTTGAAGAACTGT1646872.265020007880746No Hit
AACATTCAACGCTGTCGGTGAG1303651.7929729324559527No Hit
TATTGCACTTGTCCCGGCCTGTAA1173151.6134899671773106No Hit
TAGCTTATCAGACTGATGTTGACT1037071.4263325578660644No Hit
TAGCTTATCAGACTGATGTTGAC1018671.4010261474359722No Hit
TATTGCACTTGTCCCGGCCTGTAT936121.287491137598793No Hit
TAGCTTATCAGACTGATGTTGA622610.8563056629282405No Hit
TATTGCACTTGTCCCGGCCTGT621380.8546139844049245No Hit
TGAGGTAGTAGGTTGTATAGTT588490.809378775761135No Hit
TGTAAACATCCTTGACTGGAAGCT503740.6928179994594881No Hit
ACCGGGTGCTGTAGGCTTA470900.6476515582353455No Hit
TATTGCACTTGTCCCGGCCTGTA457110.6286855038967057No Hit
ACCGGGTGCTGTAGGCTT414340.5698618531306712No Hit
ACCGGGTGCTGTAGGCTTT366700.5043402556910197No Hit
ACTGGACTTGGAGTCAGAAGGC361180.4967483325619921No Hit
TGTAAACATCCCCGACTGGAAGCT343550.47250094039446366No Hit
TTAATGCTAATCGTGATAGGGGTT343230.4720608289087229No Hit
TCGTACGACTCTTAGCGG342220.4706717270318538No Hit
AGCAGCATTGTACAGGGCTATGA340450.4682373603763504No Hit
CATAAAGTAGAAAGCACTACT333670.4589124982722186No Hit
TGAGGTAGTAGATTGTATAGTT330880.4550752762559166No Hit
CAACGGAATCCCAAAAGCAGCTGT303120.4168956048679081No Hit
CCCATAAAGTAGAAAGCACTAC295020.4057552828850958No Hit
TTCAAGTAATTCAGGATAGGTT290120.39901607575969084No Hit
CACCCGGCTGTGTGCACATGTGC259620.35706794977502737No Hit
TTCTCACTACTGCACTTGACTAG242370.33334318999681606No Hit
CCCCCACTGCTAAATTTGACTGGCTT217050.29851936868758067No Hit
CTCCCACTGCTTCACTTGACTAG207810.2858111495368171No Hit
CAACGGAATCCCAAAAGCAGCT198780.27339175354857076No Hit
AACATTCAACGCTGTCGGTGAGTT191430.26328294286046333No Hit
CATTGCACTTGTCTCGGTCTGA190140.261508743433571No Hit
CTTCTCACTACTGCACTTGACTAG186120.2559798428939531No Hit
ACCCCCCACTGCTAAATTTGACTG181430.24952945893106548No Hit
TCGTTTCCCGGCCAATGCACCA167300.2300957861388263No Hit
TAGCTTATCAGACTGATGTTGACA156460.21518700955935902No Hit
CCCCCCACTGCTAAATTTGACTGG150270.20667360300706175No Hit
TGTAAACATCCTACACTCTCAGCT140930.19382784901700412No Hit
CCCCCCACTGCTAAATTTGACTGGCT140790.19363530024199255No Hit
CAAAGAATTCTCCTTTTGGGCT140120.1927138168187229No Hit
TATTGCACTTGTCCCGGCCTGTAG135120.18583707485402395No Hit
TGAGGTAGTAGTTTGTACAGTT134770.18535570291649506No Hit
CCCATAAAGTAGAAAGCACT132760.18259125264668608No Hit
CGCGACCTCAGATCAGACGTGG128510.17674602197669198No Hit
CTCCCACTGCTTCACTTGACTAGCCT126200.17356896718900106No Hit
CACTAGATTGTGAGCTCCTGGA125710.17289504647646056No Hit
CACTAGATTGTGAGCTCCTGGAA124160.1707632564674039No Hit
CGCCTGTCTGAGCGTCGCT123590.16997930788342822No Hit
CAACACCAGTCGATGGGCTGT118590.1631025659187293No Hit
GGAATACCGGGTGCTGTAGGCTTT117470.16156217571863674No Hit
TATTGCACTTGTCCCGGCCTGTT114950.15809629776842846No Hit
CGCGACCTCAGATCAGACGTGGCG112890.1552630800789725No Hit
ATCCCGGACGAGCCCCCA105430.1450029810676417No Hit
AACGGAATCCCAAAAGCAGCTG105270.14478292532477133No Hit
TAGCAGCACGTAAATATTGGC102260.14064312666202258No Hit
ACTGGACTTGGAGTCAGAAGGCA101780.13998295943341146No Hit
GGAATACCGGGTGCTGTAGGCTT99450.13677839767786176No Hit
CTACGCCTGTCTGAGCGTCGCT97130.13358758940624146No Hit
AGCTCGGTCTGAGGCCCCTCAGT96270.13240478978831324No Hit
CAAAGAATTCTCCTTTTGGGCTT95970.1319921852704313No Hit
GACCCGCGGGCGCTCTCCAGTCCT93930.12918647454883417No Hit
TGTCAGTTTGTCAAATACCCCAA93100.12804493538269413No Hit
TGAGGTAGTAGTTTGTGCTGTT92480.12719221937907146No Hit
GGGAATACCGGGTGCTGTAGGCTTT91340.1256243222111201No Hit
ACTGGACTTGGAGTCAGAAGGCT90990.12514295027359118No Hit
GGGAATACCGGGTGCTGTAGGCTT88590.12184211413053568No Hit
TTCAAGTAATCCAGGATAGGCTT86000.11827996179282164No Hit
TATTGCACTTGTCCCGGCCTGTAAT85680.11783985030708091No Hit
CCCCCCACTGCTAAATTTGACTGTA85330.11735847836955197No Hit
TATTGCACTTGTCCCGGCCTGTAAA82950.11408514919435529No Hit
TGTAAACATCCCCGACTGGAAGC80450.11064677821200583No Hit
CCCCCCACTGCTAAATTTGACTGT79980.11000036446732413No Hit
TCGTTTCCCGGGCGGCGCACCA78260.1076347652314677No Hit
TATTGCACTTGTCCCGGCCTGTTT76310.10495283586523511No Hit
ATGCACCTGGGCAAGGATTCTGA73180.10064799539533359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)