FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11649996
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG150132212.886888544854436No Hit
CAACGGAATCCCAAAAGCAGCTG5587764.796362161841086No Hit
CCCCCACTGCTAAATTTGACTG5224244.484327719940849No Hit
AACATTCAACGCTGTCGGTGAGT3399852.91832718225826No Hit
TTCAAGTAATCCAGGATAGGCT2658712.2821552900104No Hit
AACATTCAACGCTGTCGGTGAG2058441.7669018942152428No Hit
TAGCAGCACGTAAATATTGGCG1992871.7106186130879357No Hit
AAGCTGCCAGTTGAAGAACTGT1988181.706592860632742No Hit
ACCGGGTGCTGTAGGCTT1578691.3550991777164558No Hit
ACCGGGTGCTGTAGGCTTA1566671.3447815776074088No Hit
ACCGGGTGCTGTAGGCTTT1312781.126850172309072No Hit
TATTGCACTTGTCCCGGCCTGTAA1061880.9114852914970958No Hit
TGAGGTAGTAGGTTGTATAGTT965840.8290474949519295No Hit
TAGCTTATCAGACTGATGTTGACT916690.786858639264769No Hit
TAGCTTATCAGACTGATGTTGAC891590.7653135674896369No Hit
TATTGCACTTGTCCCGGCCTGTAT837040.7184895170779457No Hit
TATTGCACTTGTCCCGGCCTGT796030.6832877882533178No Hit
TAGCTTATCAGACTGATGTTGA599120.5142662709927112No Hit
TATTGCACTTGTCCCGGCCTGTA474420.40722760763179666No Hit
TGTAAACATCCTTGACTGGAAGCT473090.4060859763385326No Hit
CATAAAGTAGAAAGCACTACT463970.39825764747043696No Hit
TGAGGTAGTAGATTGTATAGTT459360.39430056456671747No Hit
ACTGGACTTGGAGTCAGAAGGC433440.37205162988897167No Hit
TTCTCACTACTGCACTTGACTAG421350.36167394392238417No Hit
CTCCCACTGCTTCACTTGACTAG380010.32618895319792385No Hit
CGCCTGTCTGAGCGTCGCT371510.31889281335375563No Hit
TGTAAACATCCCCGACTGGAAGCT370080.3176653451211485No Hit
CGGCCCCGCCGGGGTCGGC369200.31690998005492876No Hit
AGCAGCATTGTACAGGGCTATGA342710.29417177482292695No Hit
CAACGGAATCCCAAAAGCAGCTGT340350.29214602305442855No Hit
CCCCCACTGCTAAATTTGACTGGCTT309430.2656052414095249No Hit
TTAATGCTAATCGTGATAGGGGTT302050.25927047528600006No Hit
CCCCCCACTGCTAAATTTGACTGG295020.2532361384501763No Hit
CCCATAAAGTAGAAAGCACTAC275700.23665244176907874No Hit
CTTCTCACTACTGCACTTGACTAG270370.23207733290208857No Hit
ACCCCCCACTGCTAAATTTGACTG268470.2304464310545686No Hit
TTCAAGTAATTCAGGATAGGTT267560.22966531490654588No Hit
CGCGACCTCAGATCAGACGTGG266980.2291674606583556No Hit
CTACGCCTGTCTGAGCGTCGCT255140.21900436704012602No Hit
AACATTCAACGCTGTCGGTGAGTT246740.21179406413530097No Hit
TCGTACGACTCTTAGCGG241960.20769105843469818No Hit
GGAATACCGGGTGCTGTAGGCTTT240510.2064464228142224No Hit
CATTGCACTTGTCTCGGTCTGA236380.2029013572193501No Hit
CAACACCAGTCGATGGGCTGT228300.19596573252042315No Hit
CACCCGGCTGTGTGCACATGTGC227520.19529620439354656No Hit
GGAATACCGGGTGCTGTAGGCTT213770.18349362523386273No Hit
CTGGACGCGAGCCGGGCCCT212370.18229190808305856No Hit
CGCGACCTCAGATCAGACGTGGCG207240.17788847309475472No Hit
GGGAATACCGGGTGCTGTAGGCTT198810.17065241910812673No Hit
CCCCCCACTGCTAAATTTGACTGGCT198380.17028332026895115No Hit
GGGAATACCGGGTGCTGTAGGCTTT191960.1647725887631206No Hit
CCCATAAAGTAGAAAGCACT187190.1606781667564521No Hit
TGAGGTAGTAGTTTGTACAGTT187130.1606266645928462No Hit
GACCCGCGGGCGCTCTCCAGTCCT164750.1414163575678481No Hit
CTCCCACTGCTTCACTTGACTAGCCT162140.13917601345099173No Hit
CAAAGAATTCTCCTTTTGGGCT152970.13130476611322442No Hit
TGTAAACATCCTACACTCTCAGCT152450.13085841402864No Hit
TACGCCTGTCTGAGCGTCGCT149600.1284120612573601No Hit
AACGGAATCCCAAAAGCAGCTG145320.12473824025347303No Hit
TGAGGTAGTAGGTTGTGTGGTT138260.11867815233584629No Hit
TGAGGTAGTAGTTTGTGCTGTT135640.11642922452505564No Hit
ATACCGGGTGCTGTAGGCTTA133000.11416312932639634No Hit
ACCACCCTGAACGCGCCCGA132980.1141459619385277No Hit
GCGGCGTCCGGTGAGCTCTCGCT131410.11279832199084018No Hit
TCCACCACGTTCCCGTGG130380.11191420151560567No Hit
GAATACCGGGTGCTGTAGGCTTT128610.11039488768923184No Hit
ATCCCGGACGAGCCCCCA128010.10987986605317289No Hit
AGCTCGGTCTGAGGCCCCTCAGT125200.10746784805763024No Hit
CGCGACCTCAGATCAGACGTGGC124310.10670389929747616No Hit
TATTGCACTTGTCCCGGCCTGTT124050.10648072325518394No Hit
TAGCAGCACGTAAATATTGGC123430.10594853423125639No Hit
TACCACCCTGAACGCGCCCGA122150.104849821407664No Hit
TTCAAGTAATCCAGGATAGGC121180.10401720309603539No Hit
ACTGGACTTGGAGTCAGAAGGCT119740.10278115116949396No Hit
TATTGCACTTGTCCCGGCCTGTAG119660.10271248161801944No Hit
ACCGGGTGCTGTAGGCTA117210.10060947660411212No Hit
TCCCACTGCTTCACTTGACTAG117010.10043780272542584No Hit
CCACCCTGAACGCGCCCGA116850.10030046362247678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)