FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8167961
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG123541215.125096704061146No Hit
CAACGGAATCCCAAAAGCAGCTG4470985.473801846017629No Hit
CCCCCACTGCTAAATTTGACTG3871174.739457007691393No Hit
AACATTCAACGCTGTCGGTGAGT2452173.0021813277511975No Hit
TAGCAGCACGTAAATATTGGCG2022372.4759790111632514No Hit
TTCAAGTAATCCAGGATAGGCT1630081.9957000284403905No Hit
AACATTCAACGCTGTCGGTGAG1617841.980714648368179No Hit
AAGCTGCCAGTTGAAGAACTGT1552941.9012578537042475No Hit
TATTGCACTTGTCCCGGCCTGTAA1245341.5246644786869086No Hit
TAGCTTATCAGACTGATGTTGACT1050121.2856574609011968No Hit
TATTGCACTTGTCCCGGCCTGTAT1029921.2609266865990179No Hit
TAGCTTATCAGACTGATGTTGAC947381.1598733147721936No Hit
ACCGGGTGCTGTAGGCTTA840641.0291919856130558No Hit
TATTGCACTTGTCCCGGCCTGT751110.9195807864410713No Hit
TAGCTTATCAGACTGATGTTGA727990.8912750685268943No Hit
TGAGGTAGTAGGTTGTATAGTT664260.8132506998992771No Hit
ACCGGGTGCTGTAGGCTT642780.7869528270274552No Hit
TATTGCACTTGTCCCGGCCTGTA543080.6648905400013541No Hit
ACCGGGTGCTGTAGGCTTT525810.6437469522687486No Hit
TGTAAACATCCTTGACTGGAAGCT508770.6228849525603758No Hit
CATAAAGTAGAAAGCACTACT439220.5377351826239131No Hit
TGAGGTAGTAGATTGTATAGTT397650.4868412079832409No Hit
ACTGGACTTGGAGTCAGAAGGC388760.475957218698767No Hit
TGTAAACATCCCCGACTGGAAGCT363840.44544776842102946No Hit
AGCAGCATTGTACAGGGCTATGA363700.44527636701497475No Hit
GCCTGTCTGAGCGTCGCT327880.4014220929801207No Hit
TTCTCACTACTGCACTTGACTAG317480.3886894171017712No Hit
CTCCCACTGCTTCACTTGACTAG307730.3767525334658185No Hit
TTCAAGTAATTCAGGATAGGTT284170.34790812541832655No Hit
CGCCTGTCTGAGCGTCGCT275430.3372077804974828No Hit
CCCATAAAGTAGAAAGCACTAC262510.3213898793101485No Hit
TCGTACGACTCTTAGCGG239910.2937207951898889No Hit
CCCCCACTGCTAAATTTGACTGGCTT238730.29227612619599924No Hit
CATTGCACTTGTCTCGGTCTGA232590.28475895024474285No Hit
CAACGGAATCCCAAAAGCAGCTGT232520.2846732495417155No Hit
CTTCTCACTACTGCACTTGACTAG219580.26883086243923054No Hit
CACCCGGCTGTGTGCACATGTGC210650.2578979013244554No Hit
AACATTCAACGCTGTCGGTGAGTT200640.24564270079154393No Hit
GGAATACCGGGTGCTGTAGGCTTT199380.24410008813705156No Hit
CCCCCCACTGCTAAATTTGACTGG186610.22846583131334738No Hit
ACCCCCCACTGCTAAATTTGACTG175790.21521895121683368No Hit
CAACACCAGTCGATGGGCTGT174010.21303970476842385No Hit
CCCATAAAGTAGAAAGCACT172760.21150933507150682No Hit
GGAATACCGGGTGCTGTAGGCTT165220.2022781450597034No Hit
ATCCCGGACGAGCCCCCA161850.19815226835681513No Hit
TGAGGTAGTAGTTTGTACAGTT156600.19172471562976368No Hit
TATTGCACTTGTCCCGGCCTGTAG156020.19101462409039416No Hit
CCCCCCACTGCTAAATTTGACTGGCT153350.1877457544177794No Hit
GACCCGCGGGCGCTCTCCAGTCCT140430.17192785323044515No Hit
TAGCTTATCAGACTGATGTTGACA139050.17023832508504877No Hit
CTACGCCTGTCTGAGCGTCGCT136860.16755711737605014No Hit
TATTGCACTTGTCCCGGCCTGTT135810.16627160683063985No Hit
CACTAGATTGTGAGCTCCTGGA134210.1643127336185861No Hit
CACTAGATTGTGAGCTCCTGGAA132520.16224367378835428No Hit
CTCCCACTGCTTCACTTGACTAGCCT129780.15888910341271217No Hit
TGTCAGTTTGTCAAATACCCCAA125390.15351444503713962No Hit
CAAAGAATTCTCCTTTTGGGCT121090.1482499732797451No Hit
AGCTCGGTCTGAGGCCCCTCAGT114860.1406226107103107No Hit
AACGGAATCCCAAAAGCAGCTG114570.14026756494062595No Hit
TAGCAGCACGTAAATATTGGC114420.1400839205769959No Hit
TGTAAACATCCTACACTCTCAGCT113810.1393371001649004No Hit
TGAGGTAGTAGTTTGTGCTGTT109030.13348496644388974No Hit
CGCGACCTCAGATCAGACGTGGCG104790.12829395243194722No Hit
ACTGGACTTGGAGTCAGAAGG104530.12797563553498847No Hit
GGGAATACCGGGTGCTGTAGGCTT103730.1269961989289616No Hit
TTCACAGTGGCTAAGTTCCG101650.12444966375329168No Hit
GGGAATACCGGGTGCTGTAGGCTTT100340.12284583631092265No Hit
ACTGGACTTGGAGTCAGAAGGCA98840.12100939267462223No Hit
TACGCCTGTCTGAGCGTCGCT97390.11923416382619849No Hit
ACTGGACTTGGAGTCAGAAGGCT97100.11887911805651373No Hit
TATTGCACTTGTCCCGGCCTGTAAA95130.11646725541417252No Hit
TGTAAACATCCCCGACTGGAAGC93770.1148022131839268No Hit
CGCGACCTCAGATCAGACGTGG92660.1134432448930645No Hit
CCCCCCACTGCTAAATTTGACTGA88670.10855830482050538No Hit
CTGGACGCGAGCCGGGCCCT86480.10587709711150678No Hit
TATTGCACTTGTCCCGGCCTGTTT85860.10511803374183594No Hit
TTAATGCTAATCGTGATAGGGGTT85060.10413859713580904No Hit
TTCAAGTAATCCAGGATAGGC84680.10367336474794628No Hit
CAAAGAATTCTCCTTTTGGGCTT83640.10240009716011131No Hit
TTCAAGTAATCCAGGATAGGCTT82180.10061262535411225No Hit
TTCACAGTGGCTAAGTTCTG82090.10050243873593423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)