FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7770984
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG125496616.1493833985503No Hit
CAACGGAATCCCAAAAGCAGCTG5047936.495869763726189No Hit
CCCCCACTGCTAAATTTGACTG3735854.8074349400281875No Hit
AAGCTGCCAGTTGAAGAACTGT2101112.7037888638041205No Hit
TTCAAGTAATCCAGGATAGGCT2032442.6154216763282485No Hit
TAGCAGCACGTAAATATTGGCG1973982.540193108105743No Hit
AACATTCAACGCTGTCGGTGAGT1918012.4681687673015413No Hit
AACATTCAACGCTGTCGGTGAG1296061.6678196738019277No Hit
TATTGCACTTGTCCCGGCCTGTAA1188451.5293430021217391No Hit
TAGCTTATCAGACTGATGTTGACT1035151.3320706875731567No Hit
TATTGCACTTGTCCCGGCCTGTAT924821.1900938156609253No Hit
TAGCTTATCAGACTGATGTTGAC920001.1838912549556144No Hit
TGAGGTAGTAGGTTGTATAGTT785501.0108115008343859No Hit
TAGCTTATCAGACTGATGTTGA776750.9995516655291016No Hit
TATTGCACTTGTCCCGGCCTGT717490.9232936266501127No Hit
TGTAAACATCCTTGACTGGAAGCT688150.8855377903235935No Hit
CATAAAGTAGAAAGCACTACT544860.7011467273642565No Hit
TATTGCACTTGTCCCGGCCTGTA543150.6989462338360238No Hit
TGAGGTAGTAGATTGTATAGTT521950.6716652614392206No Hit
AGCAGCATTGTACAGGGCTATGA435040.5598261429955331No Hit
TGTAAACATCCCCGACTGGAAGCT400770.5157261937484365No Hit
TTCAAGTAATTCAGGATAGGTT375020.4825901069928853No Hit
CAACGGAATCCCAAAAGCAGCTGT335780.43209457129238715No Hit
TCGTACGACTCTTAGCGG310680.39979492944522854No Hit
TTCTCACTACTGCACTTGACTAG301220.3876214389323154No Hit
ACTGGACTTGGAGTCAGAAGGC285420.36728939346677336No Hit
CCCATAAAGTAGAAAGCACTAC285210.3670191574194465No Hit
ACCCCCCACTGCTAAATTTGACTG266940.3435086213020127No Hit
CTTCTCACTACTGCACTTGACTAG239280.30791467335410805No Hit
CTCCCACTGCTTCACTTGACTAG233070.29992340738315765No Hit
CAACGGAATCCCAAAAGCAGCT230420.29651328583355724No Hit
CCCATAAAGTAGAAAGCACT229240.29499481661524457No Hit
TGAGGTAGTAGTTTGTACAGTT222950.2869006035786459No Hit
CATTGCACTTGTCTCGGTCTGA221520.2850604247801823No Hit
AACATTCAACGCTGTCGGTGAGTT210190.2704805466077398No Hit
CCCCCCACTGCTAAATTTGACTGG209630.2697599171482016No Hit
ACCGGGTGCTGTAGGCTTA204530.2631970417131215No Hit
CACCCGGCTGTGTGCACATGTGC202670.2608035224367982No Hit
GCCTGTCTGAGCGTCGCT201580.2594008686673399No Hit
CGCCTGTCTGAGCGTCGCT198360.25525724927499527No Hit
CCCCCACTGCTAAATTTGACTGGCTT198110.2549355396948443No Hit
ACCGGGTGCTGTAGGCTT190910.245670303786496No Hit
CAAAGAATTCTCCTTTTGGGCT182710.2351182295575438No Hit
TGTAAACATCCTACACTCTCAGCT177380.2282593813087249No Hit
CAACACCAGTCGATGGGCTGT172300.2217222426400569No Hit
TAGCTTATCAGACTGATGTTGACA164140.21122164194392884No Hit
TTCACAGTGGCTAAGTTCCG163960.21099001104622014No Hit
ACCGGGTGCTGTAGGCTTT158170.20353921716992338No Hit
TGATGTAGTAGGTTGTATAGTT157470.20263843034550064No Hit
TATTGCACTTGTCCCGGCCTGTT142880.18386345924788933No Hit
CCCCCCACTGCTAAATTTGACTGGCT142550.18343880260209003No Hit
CGCGACCTCAGATCAGACGTGGCG137190.17654134920365297No Hit
TGAGGTAGTAGTTTGTGCTGTT135200.17398054094565116No Hit
TATTGCACTTGTCCCGGCCTGTAG128900.1658734595258464No Hit
CACTAGATTGTGAGCTCCTGGA127050.16349280863272914No Hit
TTCACAGTGGCTAAGTTCTG125680.16172984013350175No Hit
CACTAGATTGTGAGCTCCTGGAA121100.15583612062513577No Hit
CGCGACCTCAGATCAGACGTGG109440.14083158580689395No Hit
AACGGAATCCCAAAAGCAGCTG108920.1401624298801799No Hit
TAGCAGCACGTAAATATTGGC107740.13864396066186727No Hit
TGATGTAGTAGATTGTATAGTT105900.13627617815195606No Hit
ATCCCGGACGAGCCCCCA103090.132660162471059No Hit
AGCAGCATTGTACAGGGCTATGT100840.12976477624970015No Hit
TCGTTTCCCGGCCAATGCACCA100660.12953314535199145No Hit
CCCCCCACTGCTAAATTTGACTGTA100490.12931438283748878No Hit
CCCCCCACTGCTAAATTTGACTGA99710.12831064894741773No Hit
TTATAAAGCAATGAGACTGATT97220.12510642152911394No Hit
GACCCGCGGGCGCTCTCCAGTCCT96400.12405121410621872No Hit
CCCATAAAGTAGAAAGCACTA95890.12339492656271071No Hit
CTACGCCTGTCTGAGCGTCGCT95150.12244266620546382No Hit
AGCTCGGTCTGAGGCCCCTCAGT93580.12042233004211564No Hit
TTCAAGTAATCCAGGATAGGC93130.11984325279784387No Hit
CAAAGAATTCTCCTTTTGGGCTT92180.11862075639327015No Hit
ACTGGACTTGGAGTCAGAAGGCA92030.11842773064517957No Hit
CCCCCCACTGCTAAATTTGACTGT89920.11571250178870526No Hit
AGCAGCATTGTACAGGGCTATG89780.11553234442382072No Hit
CTCCCACTGCTTCACTTGACTAGCCT88000.11324177221314571No Hit
TATTGCACTTGTCCCGGCCTGTAAT87920.11313882514749742No Hit
TGAGGTAGTAGGTTGTGTGGTT86230.11096406838567677No Hit
TGTCAGTTTGTCAAATACCCCAA85390.10988312419636947No Hit
ACTGGACTTGGAGTCAGAAGG85280.10974157198110304No Hit
ACTGGACTTGGAGTCAGAAGGCT83090.10692339605898042No Hit
TGTCAGTTTGTCAAATACCCCA82620.10631858204829658No Hit
TTCAAGTAATCCAGGATAGGCTT81650.10507034887731077No Hit
CAACGGAATCCCAAAAGCAGCTGA81630.1050446121108987No Hit
TATTGCACTTGTCCCGGCCTGTAAA78550.1010811500834386No Hit
TATTGCACTTGTCCCGGCCTGTTT78350.1008237824193178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)