FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7114435
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG146293120.56285565895254No Hit
CAACGGAATCCCAAAAGCAGCTG5369117.546783405850219No Hit
CCCCCACTGCTAAATTTGACTG4111225.778702033260547No Hit
AACATTCAACGCTGTCGGTGAGT1797652.5267642476176957No Hit
TAGCAGCACGTAAATATTGGCG1648482.317091940540605No Hit
AAGCTGCCAGTTGAAGAACTGT1508532.1203792008782143No Hit
AACATTCAACGCTGTCGGTGAG1421461.9979942187960114No Hit
TTCAAGTAATCCAGGATAGGCT1360071.9117048648276358No Hit
TATTGCACTTGTCCCGGCCTGTAA902511.2685617339957425No Hit
TAGCTTATCAGACTGATGTTGACT743851.045550349395279No Hit
TAGCTTATCAGACTGATGTTGAC681440.9578272905719147No Hit
TATTGCACTTGTCCCGGCCTGTAT605640.851283341544339No Hit
TAGCTTATCAGACTGATGTTGA506230.7115533419027653No Hit
TGTAAACATCCTTGACTGGAAGCT495860.6969773425437157No Hit
TATTGCACTTGTCCCGGCCTGT463430.6513939617130524No Hit
TGAGGTAGTAGGTTGTATAGTT438320.6160995216064241No Hit
TATTGCACTTGTCCCGGCCTGTA401430.5642471960176739No Hit
TTAATGCTAATCGTGATAGGGGTT341310.47974294515305854No Hit
TGTAAACATCCCCGACTGGAAGCT322900.4538659781135114No Hit
AGCAGCATTGTACAGGGCTATGA321160.45142024630206057No Hit
CAACGGAATCCCAAAAGCAGCTGT312730.43957109735347927No Hit
CATAAAGTAGAAAGCACTACT278630.39164037622102105No Hit
ACCCCCCACTGCTAAATTTGACTG263470.37033158641550595No Hit
ACTGGACTTGGAGTCAGAAGGC240350.3378342763691003No Hit
TGAGGTAGTAGATTGTATAGTT237100.3332660991350684No Hit
TTCAAGTAATTCAGGATAGGTT234630.3297942844372041No Hit
CCCCCACTGCTAAATTTGACTGGCTT228410.3210514960077645No Hit
CCCCCCACTGCTAAATTTGACTGG220330.30969430460746356No Hit
TTCTCACTACTGCACTTGACTAG204840.2879216691135698No Hit
AACATTCAACGCTGTCGGTGAGTT196710.27649419806351455No Hit
CGCCTGTCTGAGCGTCGCT191340.2689461636798987No Hit
CAACGGAATCCCAAAAGCAGCT174290.2449808031136696No Hit
CAAAGAATTCTCCTTTTGGGCT168100.23628018247408264No Hit
CTTCTCACTACTGCACTTGACTAG155420.21845726329638265No Hit
CACCCGGCTGTGTGCACATGTGC154540.2172203414607063No Hit
CGCCCCACTGCTAAATTTGACTG145000.2038109842875787No Hit
CATTGCACTTGTCTCGGTCTGA144120.20257406245190235No Hit
TAGCTTATCAGACTGATGTTGACA141930.1994958137926624No Hit
CCCATAAAGTAGAAAGCACTAC139980.19675490745224322No Hit
CCCATAAAGTAGAAAGCACT135910.19103414396224014No Hit
CGCGACCTCAGATCAGACGTGGCG135120.18992372549612163No Hit
CTCCCACTGCTTCACTTGACTAG124930.17560073287618763No Hit
ACCGGGTGCTGTAGGCTT124760.17536178206702288No Hit
CTACGCCTGTCTGAGCGTCGCT122540.1722413656179303No Hit
TTCACAGTGGCTAAGTTCCG118120.16602864457964686No Hit
CAACACCAGTCGATGGGCTGT117830.16562102261107173No Hit
CACTAGATTGTGAGCTCCTGGAA114040.16029382515969295No Hit
CCCCCCACTGCTAAATTTGACTGGCT110330.1550790751479211No Hit
TGAGGTAGTAGTTTGTACAGTT109710.15420760749096732No Hit
CGCGACCTCAGATCAGACGTGG106090.14911936084875327No Hit
CACTAGATTGTGAGCTCCTGGA105570.14838845249130816No Hit
ACCGGGTGCTGTAGGCTTA104860.14739048146479658No Hit
TCGTTTCCCGGCCAATGCACCA103350.14526803604221558No Hit
CCCCCCACTGCTAAATTTGACTGTA103270.14515558860260866No Hit
TATTGCACTTGTCCCGGCCTGTAG101570.14276608051096118No Hit
AACGGAATCCCAAAAGCAGCTG101320.14241468226218948No Hit
TTCACAGTGGCTAAGTTCTG100710.14155727053518655No Hit
TGTAAACATCCTACACTCTCAGCT98160.13797300839771537No Hit
TGTCAGTTTGTCAAATACCCCAA97750.13739671526972977No Hit
TAGCAGCACGTAAATATTGGC96920.1362300730838078No Hit
CCCCCCACTGCTAAATTTGACTGT96620.13580839518528176No Hit
GCCTGTCTGAGCGTCGCT95950.13486664787857364No Hit
TATTGCACTTGTCCCGGCCTGTT95120.13370000569265164No Hit
GCCCCCACTGCTAAATTTGACTG94710.13312371256466607No Hit
TGAGGTAGTAGTTTGTGCTGTT93680.1316759517797267No Hit
ACCGGGTGCTGTAGGCTTT92400.12987679274601568No Hit
ACTGGACTTGGAGTCAGAAGGCA92360.1298205690262122No Hit
TCGTACGACTCTTAGCGG90010.12651742548775835No Hit
CCCGCCACTGCTAAATTTGACTG89300.12551945446124674No Hit
TACGCCTGTCTGAGCGTCGCT84660.11899750296404422No Hit
ACTGGACTTGGAGTCAGAAGGCT80900.11371247330251805No Hit
CCCCCCACTGCTAAATTTGACTGA78750.11069044836308155No Hit
GTGCGGTAACGCGACCGATCCCGGA76160.10704996250580687No Hit
CTCCCACTGCTTCACTTGACTAGCCT75470.10608010333919701No Hit
GACCCGCGGGCGCTCTCCAGTCCT74500.10471667813396286No Hit
TATTGCACTTGTCCCGGCCTGTAAA74060.10409821721612468No Hit
ACTGGACTTGGAGTCAGAAGG72580.1020179395833963No Hit
TGATGTAGTAGGTTGTATAGTT72220.10151192610516506No Hit
TATTGCACTTGTCCCGGCCTGTAAT71490.10048584321875173No Hit
CAAAGAATTCTCCTTTTGGGCTT71160.10002199753037311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)