FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5470808
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4925449.003130798960592No Hit
CAACGGAATCCCAAAAGCAGCTG2817295.149678073147513No Hit
CCCCCACTGCTAAATTTGACTG2010373.67472227137198No Hit
AAGCTGCCAGTTGAAGAACTGT1856763.393941077807885No Hit
TTCAAGTAATCCAGGATAGGCT1764053.2244779930130982No Hit
TAGCAGCACGTAAATATTGGCG1507052.7547119182395No Hit
AACATTCAACGCTGTCGGTGAGT1486922.7179166221881665No Hit
AACATTCAACGCTGTCGGTGAG941011.720056708259548No Hit
TGAGGTAGTAGGTTGTATAGTT624471.1414584463574668No Hit
TATTGCACTTGTCCCGGCCTGT566771.035989564978336No Hit
CATAAAGTAGAAAGCACTACT551201.0075294179580054No Hit
ACCGGGTGCTGTAGGCTT491250.8979477985701564No Hit
TAGCTTATCAGACTGATGTTGA483580.8839279316693257No Hit
TATTGCACTTGTCCCGGCCTGTAA461300.8432026859652176No Hit
TAGCTTATCAGACTGATGTTGAC441320.8066815724477993No Hit
CGCCTGTCTGAGCGTCGCT386390.7062759285282905No Hit
TAGCTTATCAGACTGATGTTGACT381230.6968440493616299No Hit
GCCTGTCTGAGCGTCGCT369630.675640600072238No Hit
TATTGCACTTGTCCCGGCCTGTAT345930.6323197597137388No Hit
TGAGGTAGTAGATTGTATAGTT341250.6237652646556048No Hit
ACCGGGTGCTGTAGGCTTT335180.6126700114498627No Hit
ACCGGGTGCTGTAGGCTTA308980.5647794621927876No Hit
TATTGCACTTGTCCCGGCCTGTA286180.5231037170377758No Hit
TGTAAACATCCTTGACTGGAAGCT284000.5191189308782177No Hit
TCGTACGACTCTTAGCGG234990.4295343576305365No Hit
ATCCCGGACGAGCCCCCA233450.4267194169490137No Hit
TTCAAGTAATTCAGGATAGGTT225530.41224257915832546No Hit
AGCAGCATTGTACAGGGCTATGA219540.4012935566373377No Hit
CCCATAAAGTAGAAAGCACT215290.39352505150975875No Hit
TTAATGCTAATCGTGATAGGGGTT214570.39220897534696886No Hit
TTCACAGTGGCTAAGTTCCG203470.371919467837292No Hit
CAACGGAATCCCAAAAGCAGCTGT203380.3717549583169433No Hit
CAACGGAATCCCAAAAGCAGCT188490.3445377721170255No Hit
TTCTCACTACTGCACTTGACTAG185710.33945625582180916No Hit
CAACACCAGTCGATGGGCTGT184750.33770148760475605No Hit
CCCATAAAGTAGAAAGCACTAC180280.3295308480941024No Hit
TGTAAACATCCCCGACTGGAAGCT178460.32620410001593914No Hit
CATTGCACTTGTCTCGGTCTGA170290.3112702913353932No Hit
ACTGGACTTGGAGTCAGAAGGC169390.3096251961319059No Hit
TGAGGTAGTAGTTTGTACAGTT153470.28052528986577485No Hit
TCCTGTCTGAGCGTCGCT150610.27529754288580405No Hit
AACATTCAACGCTGTCGGTGAGTT133520.2440590128551395No Hit
TCGTTTCCCGGCCAATGCACCA130120.23784420875307632No Hit
CTCCCACTGCTTCACTTGACTAG127470.2330003173205859No Hit
CTACGCCTGTCTGAGCGTCGCT125060.2285951179423588No Hit
TGAGGTAGTAGTTTGTGCTGTT115430.21099259926504457No Hit
TGATGTAGTAGGTTGTATAGTT115240.21064530138875281No Hit
ACGCCTGTCTGAGCGTCGCT112430.20550894858675353No Hit
TTCAAGTAATCCAGGATAGGC110470.20192629681027008No Hit
CACCCGGCTGTGTGCACATGTGC107900.1972286360625341No Hit
TTCACAGTGGCTAAGTTCTG107300.19613190592687588No Hit
TGTAAACATCCTACACTCTCAGCT106440.19455992606576578No Hit
CTTCTCACTACTGCACTTGACTAG99480.1818378564921306No Hit
TACGCCTGTCTGAGCGTCGCT93910.1716565450661036No Hit
CCCCCCACTGCTAAATTTGACTGG89350.1633213960351012No Hit
TATTGCACTTGTCCCGGCCTGTT83640.15288418091075395No Hit
TGAGGTAGTAGGTTGTGTGGTT83030.15176917193950143No Hit
AACGGAATCCCAAAAGCAGCTG78920.1442565705102427No Hit
ACCTGGTGCTGTAGGCTT76760.14030834202187317No Hit
GGAATACCGGGTGCTGTAGGCTTT75190.13743856483356753No Hit
ACCCCCCACTGCTAAATTTGACTG74520.13621388284874922No Hit
TGAGGTAGTAGGTTGTATAGT73210.13381935538589546No Hit
AGCTCGGTCTGAGGCCCCTCAGT70900.12959694436361135No Hit
TGAGAACTGAATTCCATGGGTT70830.12946899251445126No Hit
CAAAGAATTCTCCTTTTGGGCT70290.12848193539235886No Hit
CGGCCCCGCCGGGGTCGGC69320.12670888833971145No Hit
ACTGGACTTGGAGTCAGAAGG69190.12647126347698548No Hit
CCCCCACTGCTAAATTTGACTGGCTT68870.12588634073796778No Hit
CCCATAAAGTAGAAAGCACTA67820.12396706300056592No Hit
GGAATACCGGGTGCTGTAGGCTT67690.12372943813783997No Hit
CGCGACCTCAGATCAGACGTGG67370.12314451539882226No Hit
AGCAGCATTGTACAGGGCTATG67290.12299828471406783No Hit
TAGCAGCACGTAAATATTGGC66120.12085966094953432No Hit
CACTAGATTGTGAGCTCCTGGA65950.12054892074443116No Hit
TTCACAGTGGCTAAGTTCTGC65530.11978120964947044No Hit
TATTGCACTTGTCCCGGCCTG63970.1169297112967591No Hit
TGATGTAGTAGATTGTATAGTT63030.11521150075089458No Hit
TTCACAGTGGCTAAGTTCCGC59980.10963645589463203No Hit
TTATAAAGCAATGAGACTGATT57270.10468289144857579No Hit
ATCCCACCGCTGCCACCA57190.10453666076382137No Hit
TAGCTTATCAGACTGATGTTGACA56800.10382378617564353No Hit
TGTCAGTTTGTCAAATACCCCAA56140.10261738302641951No Hit
CGCGACCTCAGATCAGACGTGGCG56010.10237975816369356No Hit
TGTCAGTTTGTCAAATACC55590.1016120470687328No Hit
TATTGCACTTGTCCCGGCCTGA55560.1015572105619499No Hit
TCCCCGGCACCTCCACCA55300.10108196083649802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)