FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6532028
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG131649220.154414524861192No Hit
CAACGGAATCCCAAAAGCAGCTG3820015.848122512640791No Hit
CCCCCACTGCTAAATTTGACTG3802915.821943812855671No Hit
AACATTCAACGCTGTCGGTGAGT1867472.858943654252554No Hit
AACATTCAACGCTGTCGGTGAG1439402.203603536298375No Hit
TAGCAGCACGTAAATATTGGCG1412802.1628811144104096No Hit
AAGCTGCCAGTTGAAGAACTGT1269291.9431790555704906No Hit
TTCAAGTAATCCAGGATAGGCT1210421.8530539060763365No Hit
TATTGCACTTGTCCCGGCCTGTAA770311.179281534004447No Hit
TAGCTTATCAGACTGATGTTGACT542720.8308598799637723No Hit
TATTGCACTTGTCCCGGCCTGTAT520870.7974093191272297No Hit
TAGCTTATCAGACTGATGTTGA486300.7444854798540361No Hit
TATTGCACTTGTCCCGGCCTGT485290.7429392525567864No Hit
TTAATGCTAATCGTGATAGGGGTT485000.7424952863031206No Hit
TAGCTTATCAGACTGATGTTGAC482150.7381321696722671No Hit
TGAGGTAGTAGGTTGTATAGTT477200.7305541249976271No Hit
TATTGCACTTGTCCCGGCCTGTA426400.6527834847003106No Hit
TGTAAACATCCTTGACTGGAAGCT357790.5477471927554506No Hit
CGCCTGTCTGAGCGTCGCT351940.5387913217763304No Hit
ACCGGGTGCTGTAGGCTT304370.4659655469939811No Hit
AGCAGCATTGTACAGGGCTATGA292110.44719649089073105No Hit
TGAGGTAGTAGATTGTATAGTT260940.3994777732122397No Hit
TGTAAACATCCCCGACTGGAAGCT257060.39353781092181483No Hit
CATAAAGTAGAAAGCACTACT256010.39193034689992146No Hit
ACCGGGTGCTGTAGGCTTA234090.35837262179525253No Hit
ACCGGGTGCTGTAGGCTTT223930.34281849373578926No Hit
AACATTCAACGCTGTCGGTGAGTT205940.3152772768273498No Hit
CAACGGAATCCCAAAAGCAGCTGT205500.31460367285627067No Hit
ACTGGACTTGGAGTCAGAAGGC202790.3104548847616697No Hit
TTCAAGTAATTCAGGATAGGTT191810.29364540384701354No Hit
GCCTGTCTGAGCGTCGCT178020.2725340430261475No Hit
TTCTCACTACTGCACTTGACTAG169290.25916912787269125No Hit
ACCCCCCACTGCTAAATTTGACTG168530.25800563010446376No Hit
CAACACCAGTCGATGGGCTGT157950.24180851643624307No Hit
GGAATACCGGGTGCTGTAGGCTTT145960.22345280822433705No Hit
CCCCCCACTGCTAAATTTGACTGG144960.22192189010824814No Hit
CAACGGAATCCCAAAAGCAGCT143840.22020726181822858No Hit
CATTGCACTTGTCTCGGTCTGA140680.21536956057138765No Hit
CCCATAAAGTAGAAAGCACT135330.20717914865031198No Hit
TCGTACGACTCTTAGCGG128240.1963249392072416No Hit
TTCACAGTGGCTAAGTTCCG127900.1958044270477714No Hit
CAAAGAATTCTCCTTTTGGGCT126670.19392139776498202No Hit
GGAATACCGGGTGCTGTAGGCTT119030.18222518335806276No Hit
CTTCTCACTACTGCACTTGACTAG118570.18152096102466186No Hit
TTCACAGTGGCTAAGTTCTG118370.1812147774014441No Hit
CTACGCCTGTCTGAGCGTCGCT113550.17383575208189556No Hit
CACCCGGCTGTGTGCACATGTGC111840.1712178821033835No Hit
CCCCCACTGCTAAATTTGACTGGCTT110510.16918176100898527No Hit
TGAGGTAGTAGTTTGTACAGTT107210.16412973122589186No Hit
TACGCCTGTCTGAGCGTCGCT99400.15217326073923748No Hit
TCGTTTCCCGGCCAATGCACCA98350.15056579671734416No Hit
ATCCCGGACGAGCCCCCA95520.14623329844881253No Hit
CGCGACCTCAGATCAGACGTGGCG94880.14525351085451563No Hit
CACTAGATTGTGAGCTCCTGGA94700.14497794559361962No Hit
TATTGCACTTGTCCCGGCCTGTT94510.14468707115156274No Hit
TATTGCACTTGTCCCGGCCTGTAG93620.1433245540282436No Hit
TGAGGTAGTAGTTTGTGCTGTT92730.14196203690492445No Hit
CCCATAAAGTAGAAAGCACTAC92580.14173239918751113No Hit
TGATGTAGTAGGTTGTATAGTT92070.1409516309483058No Hit
CACTAGATTGTGAGCTCCTGGAA92000.14084446668017955No Hit
TAGCTTATCAGACTGATGTTGACA91570.14018617189026134No Hit
CTCCCACTGCTTCACTTGACTAG90630.13874710886113778No Hit
ACGCCTGTCTGAGCGTCGCT86030.1317048855271288No Hit
ACTGGACTTGGAGTCAGAAGGCA85790.13133746517926745No Hit
AACGGAATCCCAAAAGCAGCTG85580.1310159723748888No Hit
TGTAAACATCCTACACTCTCAGCT83680.12810722795431986No Hit
CGCGACCTCAGATCAGACGTGG82760.12669878328751805No Hit
ACTGGACTTGGAGTCAGAAGG82140.12574961405554294No Hit
CCCCCCACTGCTAAATTTGACTGA81750.12515255599026825No Hit
GACCCGCGGGCGCTCTCCAGTCCT81660.12501477335982025No Hit
AGCAGCATTGTACAGGGCTATG78000.11941161305493485No Hit
TAGCAGCACGTAAATATTGGC77910.11927383042448685No Hit
CGCCCCACTGCTAAATTTGACTG71800.10991992073518361No Hit
TATTGCACTTGTCCCGGCCTG71590.10959842793080495No Hit
TCCTGTCTGAGCGTCGCT69740.10676622941604046No Hit
TGTCAGTTTGTCAAATACCCCAA68780.10529654802459512No Hit
TGAGGTAGTAGGTTGTGTGGTT68490.10485258177092933No Hit
CCCGCCACTGCTAAATTTGACTG68190.10439330633610266No Hit
CCAAGTCCTTCTGATCGAGGCC65810.10074972121981106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)