FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6666239
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82650912.39843035930755No Hit
CAACGGAATCCCAAAAGCAGCTG5023807.536183446168071No Hit
CCCCCACTGCTAAATTTGACTG3111504.667549423295505No Hit
AACATTCAACGCTGTCGGTGAGT2165973.2491634338342803No Hit
TTCAAGTAATCCAGGATAGGCT1762472.6438746045558825No Hit
AACATTCAACGCTGTCGGTGAG1652272.4785639998805924No Hit
AAGCTGCCAGTTGAAGAACTGT1650432.4758038228152337No Hit
TAGCAGCACGTAAATATTGGCG1477852.2169172152393575No Hit
TATTGCACTTGTCCCGGCCTGT632450.9487358614055091No Hit
TATTGCACTTGTCCCGGCCTGTAA550730.8261479973940328No Hit
TAGCTTATCAGACTGATGTTGA493190.7398324602523252No Hit
TGAGGTAGTAGGTTGTATAGTT482420.7236764238425895No Hit
TAGCTTATCAGACTGATGTTGAC481880.7228663718777559No Hit
CATAAAGTAGAAAGCACTACT467730.7016400102066548No Hit
TAGCTTATCAGACTGATGTTGACT415240.6228999590323719No Hit
TATTGCACTTGTCCCGGCCTGTAT383560.575376910428804No Hit
CGCCTGTCTGAGCGTCGCT380240.5703965909413089No Hit
ACCGGGTGCTGTAGGCTT380050.570111572657386No Hit
TATTGCACTTGTCCCGGCCTGTA374620.5619660501221153No Hit
TCGTACGACTCTTAGCGG352910.5293989609433445No Hit
CAACGGAATCCCAAAAGCAGCTGT288910.43339280214825776No Hit
TGTAAACATCCTTGACTGGAAGCT283290.4249622613290643No Hit
AGCAGCATTGTACAGGGCTATGA257570.3863797862632888No Hit
TGAGGTAGTAGATTGTATAGTT253390.3801093840169847No Hit
ACCGGGTGCTGTAGGCTTT238730.35811797326798517No Hit
AACATTCAACGCTGTCGGTGAGTT236990.3555078058257437No Hit
TTCAAGTAATTCAGGATAGGTT234900.3523726047025917No Hit
ACCGGGTGCTGTAGGCTTA223570.335376514403399No Hit
CCCATAAAGTAGAAAGCACT217440.32618092450630704No Hit
TGTAAACATCCCCGACTGGAAGCT205370.3080747629960462No Hit
CAACACCAGTCGATGGGCTGT197850.2967940393376235No Hit
TTCTCACTACTGCACTTGACTAG196720.29509893059639775No Hit
CTCCCACTGCTTCACTTGACTAG195390.29310380260893737No Hit
ACTGGACTTGGAGTCAGAAGGC192170.28827349274455955No Hit
CATTGCACTTGTCTCGGTCTGA188840.2832781722947527No Hit
CTACGCCTGTCTGAGCGTCGCT185740.2786278739781157No Hit
TTCACAGTGGCTAAGTTCCG183850.2757926921011983No Hit
CAACGGAATCCCAAAAGCAGCT181680.2725374832795524No Hit
CCCATAAAGTAGAAAGCACTAC177640.2664770945056125No Hit
ACCCCCCACTGCTAAATTTGACTG172300.25846658063114747No Hit
CCCCCACTGCTAAATTTGACTGGCTT166430.24966101575416064No Hit
CCCCCCACTGCTAAATTTGACTGG152710.22907969546246393No Hit
CACCCGGCTGTGTGCACATGTGC151450.22718957421118566No Hit
ATCCCGGACGAGCCCCCA149710.22457940676894422No Hit
TGATGTAGTAGGTTGTATAGTT141010.21152856955773713No Hit
TCGTTTCCCGGCCAATGCACCA129150.19373742825602264No Hit
ACGCCTGTCTGAGCGTCGCT121660.18250170748453512No Hit
TTCACAGTGGCTAAGTTCTG120740.18112161895185575No Hit
CTTCTCACTACTGCACTTGACTAG116880.17533124749952708No Hit
TGAGGTAGTAGTTTGTACAGTT110610.16592564413007097No Hit
TATTGCACTTGTCCCGGCCTGTT110250.16538560948684858No Hit
ACCTGGTGCTGTAGGCTT104320.15649003883599133No Hit
AACGGAATCCCAAAAGCAGCTG103960.15595000419276897No Hit
TGAGGTAGTAGTTTGTGCTGTT103460.15519995607718237No Hit
TACGCCTGTCTGAGCGTCGCT103060.15459991758471306No Hit
TTCAAGTAATCCAGGATAGGC99650.14948458943641235No Hit
CCCCCCACTGCTAAATTTGACTGGCT99230.1488545490193196No Hit
TGTAAACATCCTACACTCTCAGCT98710.14807449897910951No Hit
CAAAGAATTCTCCTTTTGGGCT96150.14423425262730605No Hit
TGAGGTAGTAGGTTGTGTGGTT95390.14309417949161438No Hit
TAGCAGCACGTAAATATTGGC90580.13587871661967116No Hit
GCCTGTCTGAGCGTCGCT88400.1326085068357135No Hit
AGCAGCATTGTACAGGGCTATG78730.11810257628026836No Hit
AGCTCGGTCTGAGGCCCCTCAGT77450.11618245310436665No Hit
TATTGCACTTGTCCCGGCCTG76450.1146823568731934No Hit
CCCATAAAGTAGAAAGCACTA76190.11429233185308839No Hit
TAGCTTATCAGACTGATGTTGACA74050.11108212591837766No Hit
TGTCAGTTTGTCAAATACCCCAA73410.11012206433042679No Hit
TGATGTAGTAGATTGTATAGTT72590.10889198542086474No Hit
GACCCGCGGGCGCTCTCCAGTCCT71100.10665684203641664No Hit
CCCCCCACTGCTAAATTTGACTGA70440.10566677852384229No Hit
TCCCACTGCTTCACTTGACTAG70240.10536675927760766No Hit
CACTAGATTGTGAGCTCCTGGA69980.10497673425750262No Hit
CGGCCCCGCCGGGGTCGGC69160.10374665534794057No Hit
TAGCAGCACATCATGGTTTACA67190.1007914657725293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)