FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9970670
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6613766.633215220240967No Hit
CAACGGAATCCCAAAAGCAGCTG5641295.657884575459823No Hit
GCCTGTCTGAGCGTCGCT4668164.6818919892043365No Hit
AACATTCAACGCTGTCGGTGAGT3671203.68199930395851No Hit
GGAATACCGGGTGCTGTAGGCTTT2640662.648427838851351No Hit
TAGCAGCACGTAAATATTGGCG2458882.4661131097508995No Hit
AAGCTGCCAGTTGAAGAACTGT2154552.160887884164254No Hit
AACATTCAACGCTGTCGGTGAG2126132.132384283102339No Hit
TTCAAGTAATCCAGGATAGGCT2055912.061957721998622No Hit
GGAATACCGGGTGCTGTAGGCTT2053762.059801397498864No Hit
CCCCCACTGCTAAATTTGACTG1968401.974190300150341No Hit
TATTGCACTTGTCCCGGCCTGTAT1508411.5128471807812314No Hit
TATTGCACTTGTCCCGGCCTGTAA1447671.4519285063090044No Hit
TATTGCACTTGTCCCGGCCTGT1432661.4368743524758116No Hit
TGAGGTAGTAGGTTGTATAGTT1226041.2296465533409489No Hit
CGCCTGTCTGAGCGTCGCT971340.9741973207417355No Hit
TCGTACGACTCTTAGCGG886260.8888670470489947No Hit
TATTGCACTTGTCCCGGCCTGTA804850.8072175691302591No Hit
TGAGGTAGTAGATTGTATAGTT796550.7988931536195663No Hit
ACTGGACTTGGAGTCAGAAGGC781090.783387676053866No Hit
TAGCTTATCAGACTGATGTTGACT746840.7490369253019105No Hit
TAGCTTATCAGACTGATGTTGAC718700.7208141478957784No Hit
ATCCCGGACGAGCCCCCA705440.7075151419112257No Hit
AACCGGGCGGAAACACCA635030.6368980218982275No Hit
TAGCTTATCAGACTGATGTTGA609530.6113230103894723No Hit
AGCAGCATTGTACAGGGCTATGA556350.5579865746233703No Hit
CATAAAGTAGAAAGCACTACT517760.5192830572067875No Hit
TGTAAACATCCTTGACTGGAAGCT505900.5073881695011468No Hit
TCCTGTCTGAGCGTCGCT478840.48024856905303254No Hit
GGAATACCGGGTGCTGTAGGCTTA435320.43660054941142373No Hit
TGTAAACATCCCCGACTGGAAGCT419280.4205133657016028No Hit
TTCAAGTAATTCAGGATAGGTT417150.4183771000344009No Hit
CACCCGGCTGTGTGCACATGTGC416930.4181564528762861No Hit
TTCTCACTACTGCACTTGACTAG357420.3584713966062461No Hit
ACCGGGTGCTGTAGGCTTA332730.33370876781600434No Hit
AGCTCGGTCTGAGGCCCCTCAGT324140.32509349923325115No Hit
ACCGGGTGCTGTAGGCTT320130.3210717033057959No Hit
TGAGGTAGTAGTTTGTACAGTT319060.319998555764056No Hit
GGAATACCGGGTGCTGTAGGCT315600.31652837773188763No Hit
CAACGGAATCCCAAAAGCAGCTGT307460.30836443288164184No Hit
CATTGCACTTGTCTCGGTCTGA306160.3070606087655092No Hit
CTCCCACTGCTTCACTTGACTAG303510.30440281345185427No Hit
TGAATACCGGGTGCTGTAGGCTTT283450.2842838043983002No Hit
ACCGGGTGCTGTAGGCTTT260320.26108576454741755No Hit
CCCATAAAGTAGAAAGCACTAC253350.25409526140169114No Hit
TACGCCTGTCTGAGCGTCGCT246990.24771655264891929No Hit
CTTCTCACTACTGCACTTGACTAG243190.24390537446330085No Hit
AACATTCAACGCTGTCGGTGAGTT237500.2381986366011512No Hit
TATTGCACTTGTCCCGGCCTGTT236980.23767710695469813No Hit
TGAGGTAGTAGTTTGTGCTGTT218280.21892209851494432No Hit
TGAATACCGGGTGCTGTAGGCTT216910.21754806848486613No Hit
CCCCCCACTGCTAAATTTGACTGG211410.21203188953199734No Hit
CACTAGATTGTGAGCTCCTGGA211150.21177112470877082No Hit
TATTGCACTTGTCCCGGCCTGTAG207630.2082407701789348No Hit
CCCATAAAGTAGAAAGCACT206330.2069369460628022No Hit
ATCCCACCGCTGCCACCA197820.1984019128102725No Hit
TGAGAACTGAATTCCATGGGTT196560.1971382063592517No Hit
CTACGCCTGTCTGAGCGTCGCT196280.19685738270346928No Hit
ACGCCTGTCTGAGCGTCGCT191740.19230402771328306No Hit
CAACACCAGTCGATGGGCTGT190890.1914515273296579No Hit
TGAGGTAGTAGGTTGTGTGGTT185820.18636661327674067No Hit
CCCCCCACTGCTAAATTTGACTGGCT179460.1799879045239688No Hit
ACTGGACTTGGAGTCAGAAGGCA177800.1783230214218302No Hit
AACGGAATCCCAAAAGCAGCTG167310.1678021637462678No Hit
ACTGGACTTGGAGTCAGAAGGCT163040.16351960299558604No Hit
ACTGGACTTGGAGTCAGAAGG161500.1619750728887828No Hit
CACTAGATTGTGAGCTCCTGGAA150350.15079227373887613No Hit
TCGTTTCCCGGGCGGCGCACCA144230.14465427097677488No Hit
CCCCCACTGCTAAATTTGACTGGCTT143960.14438347673727042No Hit
TCCTTCTGATCGAGGCCC137100.1375032971706014No Hit
TGAGGTAGTAGGTTGTATAGT135150.13554756099640244No Hit
TATTGCACTTGTCCCGGCCTGTTT132740.13313047167341813No Hit
TAGCAGCACGTAAATATTGGC132500.1328897656827475No Hit
CGCGACCTCAGATCAGACGTGGCG127920.12829629302744952No Hit
CCCCCCACTGCTAAATTTGACTGTA126350.12672167467181242No Hit
CCCCCCACTGCTAAATTTGACTGT125230.1255983800486828No Hit
TGTAAACATCCCCGACTGGAAGC124510.12487626207667087No Hit
ACCACAGGGTAGAACCACGGAC122640.1230007612326955No Hit
TGTAAACATCCTACACTCTCAGCT118830.11917955363079913No Hit
TTCAAGTAATCCAGGATAGGCTT117630.11797602367744596No Hit
ACCCCCCACTGCTAAATTTGACTG115310.11564919910096313No Hit
TTCAAGTAATCCAGGATAGGC113720.11405452191277016No Hit
GCGTTCGCGCTTTCCCCTG113120.11345275693609355No Hit
TGTCAGTTTGTCAAATACCCCAA111060.11138669718283725No Hit
TTCACCACCTTCTCCACCCAGC110190.11051413796665621No Hit
TATTGCACTTGTCCCGGCCTGA109750.1100728436504267No Hit
TTAATGCTAATCGTGATAGGGGTT106670.10698378343682019No Hit
CTCCCACTGCTTCACTTGACTAGCCT105340.10564987107185375No Hit
TAGTGGGTTATCAGAACT105310.10561978282301991No Hit
CGCGACCTCAGATCAGACGTGG104640.10494781193239773No Hit
GAATACCGGGTGCTGTAGGCTTT104590.10489766485100802No Hit
CAAAGTGCTGTTCGTGCAGGT102230.10253072260941341No Hit
AGCAGCATTGTACAGGGCTATGT101420.10171833989090001No Hit
CCCCCCACTGCTAAATTTGACTGA100720.10101628075144399No Hit
AGCAGCATTGTACAGGGCTATG99930.10022395686548648No Hit
CAAAGTGCTGTTCGTGCAGGTAG99770.10006348620503938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)