FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5479170
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5236369.556848938799124No Hit
CAACGGAATCCCAAAAGCAGCTG5190189.472566100340014No Hit
AACATTCAACGCTGTCGGTGAGT1632732.979885639613299No Hit
TAGCAGCACGTAAATATTGGCG1583142.8893792307959054No Hit
TTCAAGTAATCCAGGATAGGCT1369422.49932015250485No Hit
CCCCCACTGCTAAATTTGACTG1351272.4661946973720474No Hit
AAGCTGCCAGTTGAAGAACTGT1177522.149084624131027No Hit
AACATTCAACGCTGTCGGTGAG1112992.0313113117497723No Hit
TATTGCACTTGTCCCGGCCTGTAA1071481.9555516620218025No Hit
TATTGCACTTGTCCCGGCCTGTAT1040571.8991380081289686No Hit
TAGCTTATCAGACTGATGTTGAC770421.406088878425017No Hit
TAGCTTATCAGACTGATGTTGACT561641.0250457642307138No Hit
TATTGCACTTGTCCCGGCCTGT560661.023257172162937No Hit
TATTGCACTTGTCCCGGCCTGTA528290.964178881107905No Hit
CAACGGAATCCCAAAAGCAGCT507300.925870159166443No Hit
TGAGGTAGTAGGTTGTATAGTT457210.8344512033756938No Hit
TGTAAACATCCTTGACTGGAAGCT394580.7201455694931896No Hit
TAGCTTATCAGACTGATGTTGA380700.6948132655128423No Hit
AGCAGCATTGTACAGGGCTATGA377710.6893562346121767No Hit
TGAGGTAGTAGATTGTATAGTT316190.5770764550105217No Hit
CAACGGAATCCCAAAAGCAGCTGT316040.5768026909185151No Hit
ACTGGACTTGGAGTCAGAAGGC292420.5336939718972035No Hit
CCCCCACTGCTAAATTTGACTGGCTT279340.5098217430742248No Hit
TTCTCACTACTGCACTTGACTAG268800.49058525287589183No Hit
TTCAAGTAATTCAGGATAGGTT261950.4780833593409221No Hit
TGTAAACATCCCCGACTGGAAGCT259310.4732651113216053No Hit
CACCCGGCTGTGTGCACATGTGC251080.4582445881401745No Hit
CTTCTCACTACTGCACTTGACTAG239370.4368727380241898No Hit
CATAAAGTAGAAAGCACTACT202830.37018380521137323No Hit
TTAATGCTAATCGTGATAGGGGTT188520.3440667108339402No Hit
CTCCCACTGCTTCACTTGACTAG176030.32127128743952094No Hit
CCCCCCACTGCTAAATTTGACTGG174880.3191724294008034No Hit
CCCATAAAGTAGAAAGCACTAC170260.310740495366999No Hit
CCCCCCACTGCTAAATTTGACTGGCT162040.2957382231250354No Hit
CATTGCACTTGTCTCGGTCTGA159710.2914857542291989No Hit
CAAAGAATTCTCCTTTTGGGCT145450.26545991454910145No Hit
TATTGCACTTGTCCCGGCCTGTAG135850.2479390126606767No Hit
ACCCCCCACTGCTAAATTTGACTG131990.24089415002637263No Hit
TAGCTTATCAGACTGATGTTGACA130180.23759072998282585No Hit
TATTGCACTTGTCCCGGCCTGTT125350.2287755262202122No Hit
TGAGGTAGTAGTTTGTACAGTT125090.22830100179406734No Hit
TCGTTTCCCGGCCAATGCACCA122520.22361051035102034No Hit
AACATTCAACGCTGTCGGTGAGTT121940.22255195586192797No Hit
CAACGGAATCCCAAAAGCAGCTGA115370.21056108863203735No Hit
TCGTACGACTCTTAGCGG113990.20804245898557627No Hit
CTCCCACTGCTTCACTTGACTAGCCT110120.20097934541180507No Hit
TAGCAGCACGTAAATATTGGC107470.19614284645302119No Hit
CACTAGATTGTGAGCTCCTGGA106860.1950295391455275No Hit
CTACGCCTGTCTGAGCGTCGCT106680.19470102223511954No Hit
TATTGCACTTGTCCCGGCCTGTTT106560.19448201096151424No Hit
TGTAAACATCCTACACTCTCAGCT104010.18982802139740143No Hit
CACTAGATTGTGAGCTCCTGGAA102670.1873823955088088No Hit
CCCCCCACTGCTAAATTTGACTGTA102150.18643334665651914No Hit
AGCTCGGTCTGAGGCCCCTCAGT101790.18577631283570323No Hit
AACGGAATCCCAAAAGCAGCTG100650.18369570573645277No Hit
TACGCCTGTCTGAGCGTCGCT100360.18316642849190662No Hit
TGAGGTAGTAGTTTGTGCTGTT96650.17639532994960916No Hit
ACTGGACTTGGAGTCAGAAGGCA95530.17435122472929293No Hit
GTCAGGATGGCCGAGCGGTCTAAGG93600.17082879341214088No Hit
TTCACAGTGGCTAAGTTCTGC92130.16814590531047585No Hit
TGAGAACTGAATTCCATGGGTT90390.1649702418431989No Hit
GGAATACCGGGTGCTGTAGGCTTT89490.16332765729115906No Hit
CAAAGAATTCTCCTTTTGGGCTT87580.15984172785294123No Hit
CCCCCCACTGCTAAATTTGACTGT86350.15759686229848682No Hit
TGTAAACATCCCCGACTGGAAGC84800.15476796668108492No Hit
TATTGCACTTGTCCCGGCCTGTAAT83230.1519025691847488No Hit
TTCACAGTGGCTAAGTTCCGC79760.14556949318966195No Hit
ATGCACCTGGGCAAGGATTCTGA76700.13998470571272656No Hit
TAGCAGCACATCATGGTTTACA74640.1362250121825021No Hit
CCCATAAAGTAGAAAGCACT74040.13512995581447554No Hit
TATTGCACTTGTCCCGGCCTGTAAA73800.13469193326726492No Hit
TGATGTAGTAGGTTGTATAGTT72570.13244706771281053No Hit
GCCTGTCTGAGCGTCGCT71920.13126075664744843No Hit
TATTGCACTTGTCCCGGCCTGA71170.12989193618741526No Hit
CTACGGGGATGATTTTACGAACTGAA68510.12503718628916424No Hit
ACTGGACTTGGAGTCAGAAGGCT68280.12461741468142074No Hit
TGAGGTAGTAGGTTGTGTGGTT66860.12202578127709124No Hit
TTCAAGTAATCCAGGATAGGCTT66790.12189802470082148No Hit
ACTTGACTTGGAGTCAGAAGGC64910.11846684808100497No Hit
CCCCCACTGCTAAATTTGACTGGCTA64700.11808357835219568No Hit
CAAAGTGCTGTTCGTGCAGGTAG64630.11795582177592592No Hit
AGCAGCATTGTACAGGGCTATGT64410.11755430110764953No Hit
AATGCACCCGGGCAAGGATTCT64350.11744479547084687No Hit
GGAATACCGGGTGCTGTAGGCTT64050.11689726728683358No Hit
CGCCTGTCTGAGCGTCGCT62700.11443339045877386No Hit
ATCACATTGCCAGGGATTTCC60960.11125772699149689No Hit
AACACCCTGATTGCTCCTGTCTGATT58280.10636647521431167No Hit
TAGCAGCACGTAAATATTGGCGT57950.10576419421189705No Hit
GTCCGATGGTAGTGGGTTATCAGAAC55920.10205925350007392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)