FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9603006
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG131672313.711571147617734No Hit
CAACGGAATCCCAAAAGCAGCTG9004309.37654313659702No Hit
AACATTCAACGCTGTCGGTGAGT3733313.887647263783861No Hit
CCCCCACTGCTAAATTTGACTG3688813.8413076072221553No Hit
TTCAAGTAATCCAGGATAGGCT2859852.9780779060223432No Hit
AAGCTGCCAGTTGAAGAACTGT2498472.601758241117417No Hit
TAGCAGCACGTAAATATTGGCG2189842.2803692926985573No Hit
AACATTCAACGCTGTCGGTGAG2149592.2384553336736435No Hit
TATTGCACTTGTCCCGGCCTGTAA1211661.2617507476304815No Hit
TATTGCACTTGTCCCGGCCTGTAT1058361.1021132341268975No Hit
TGAGGTAGTAGGTTGTATAGTT1045151.0883571248419506No Hit
TATTGCACTTGTCCCGGCCTGT827880.8621050533551682No Hit
TAGCTTATCAGACTGATGTTGAC708000.7372691425997234No Hit
TAGCTTATCAGACTGATGTTGACT700450.7294070210931869No Hit
CAACGGAATCCCAAAAGCAGCTGT604240.629219642266182No Hit
CATAAAGTAGAAAGCACTACT578350.6022593342126413No Hit
TGAGGTAGTAGATTGTATAGTT543030.5654791843304066No Hit
TAGCTTATCAGACTGATGTTGA517470.5388625186738403No Hit
CTCCCACTGCTTCACTTGACTAG516390.5377378708291967No Hit
TATTGCACTTGTCCCGGCCTGTA508360.5293759058361517No Hit
TTCAAGTAATTCAGGATAGGTT464520.48372353406839486No Hit
TGTAAACATCCTTGACTGGAAGCT462210.48131803728957373No Hit
AGCAGCATTGTACAGGGCTATGA461410.4804849648120599No Hit
ACTGGACTTGGAGTCAGAAGGC455410.4742369212307063No Hit
CCCCCACTGCTAAATTTGACTGGCTT445640.46406302359906887No Hit
CACCCGGCTGTGTGCACATGTGC441460.4597102199040592No Hit
CCCATAAAGTAGAAAGCACTAC437500.4555865111403658No Hit
TTAATGCTAATCGTGATAGGGGTT436870.45493046656432373No Hit
TTCTCACTACTGCACTTGACTAG421190.438602246005053No Hit
CCCCCCACTGCTAAATTTGACTGGCT416560.4337808390414418No Hit
CCCCCCACTGCTAAATTTGACTGG400300.4168486409359736No Hit
CGCCTGTCTGAGCGTCGCT375970.3915128242135848No Hit
CTTCTCACTACTGCACTTGACTAG342240.3563884058804087No Hit
TGTAAACATCCCCGACTGGAAGCT329890.34352784950878923No Hit
CAACGGAATCCCAAAAGCAGCT243630.2537018096208625No Hit
AACATTCAACGCTGTCGGTGAGTT243100.2531498991045095No Hit
CCCATAAAGTAGAAAGCACT239460.24935941933182174No Hit
TCGTACGACTCTTAGCGG234980.2446942134577444No Hit
ACCCCCCACTGCTAAATTTGACTG230940.24048719744629962No Hit
CTACGCCTGTCTGAGCGTCGCT229430.23891477314499232No Hit
AGCTCGGTCTGAGGCCCCTCAGT228940.23840451625251508No Hit
TGAGGTAGTAGTTTGTACAGTT226700.23607191331547644No Hit
CATTGCACTTGTCTCGGTCTGA225810.2351451201842423No Hit
GGAATACCGGGTGCTGTAGGCTTT225540.2348639582230814No Hit
CTCCCACTGCTTCACTTGACTAGCCT218940.22799111028359245No Hit
TGTAAACATCCTACACTCTCAGCT214230.22308639607222985No Hit
CCCCCCACTGCTAAATTTGACTGTA202280.2106423759393673No Hit
AACGGAATCCCAAAAGCAGCTG188490.196282289108223No Hit
ACCGGGTGCTGTAGGCTT175350.18259907366505862No Hit
CAAAGAATTCTCCTTTTGGGCT174290.18149525263235283No Hit
CCCCCCACTGCTAAATTTGACTGT171560.17865239280283693No Hit
TGAGAACTGAATTCCATGGGTT167760.17469529853464635No Hit
TGAGGTAGTAGGTTGTGTGGTT166610.17349775684822022No Hit
GGAATACCGGGTGCTGTAGGCTT157700.16421941212991018No Hit
TGAGGTAGTAGTTTGTGCTGTT153180.15951255263195713No Hit
CACTAGATTGTGAGCTCCTGGA151870.15814839645002823No Hit
TACGCCTGTCTGAGCGTCGCT151860.15813798304405932No Hit
TTCAAGTAATCCAGGATAGGC149860.1560553018502748No Hit
CCCCCACTGCTAAATTTGACTGGCT148220.15434750327137148No Hit
TATTGCACTTGTCCCGGCCTGTT143720.14966147058535628No Hit
TATTGCACTTGTCCCGGCCTGTAG142700.14859930317652617No Hit
TCCCACTGCTTCACTTGACTAG142600.14849516911683694No Hit
ACCGGGTGCTGTAGGCTTT139590.14536073392019125No Hit
TCGTTTCCCGGCCAATGCACCA139590.14536073392019125No Hit
ACCGGGTGCTGTAGGCTTA135450.14104958384905728No Hit
CCCCCCACTGCTAAATTTGACTGA129820.13518683628855382No Hit
CAACACCAGTCGATGGGCTGT127670.13294795400523543No Hit
ATCCCGGACGAGCCCCCA127190.13244811051872715No Hit
TTCAAGTAATCCAGGATAGGCTT125980.13118808839648752No Hit
CACTAGATTGTGAGCTCCTGGAA125740.13093816665323338No Hit
TGATGTAGTAGGTTGTATAGTT122540.1276058767431781No Hit
ACGCCTGTCTGAGCGTCGCT121140.12614799990752895No Hit
CAAAGAATTCTCCTTTTGGGCTT119070.12399242487196196No Hit
CGCGACCTCAGATCAGAC118910.1238258103764592No Hit
GTCAGGATGGCCGAGCGGTCTAAGG117880.12275322956166017No Hit
TAGCAGCACGTAAATATTGGC115250.12001450379183352No Hit
TCGTTTCCCGGGCGGCGCACCA111240.11583872799829553No Hit
CCCCCACTGCTAAATTTGACTGGCTA108050.11251685149420922No Hit
ACCACAGGGTAGAACCACGGAC105990.11037168986461113No Hit
CCCCCCACTGCTAAATTTGACTGGC102650.10689361227099099No Hit
CAAAGTGCTGTTCGTGCAGGTAG100020.10415488650116432No Hit
AACATTCATTGCTGTCGGTGGGT99800.10392579156984802No Hit
TTCACAGTGGCTAAGTTCTGC99620.10373835026240741No Hit
CAAAGTGCTGTTCGTGCAGGT99600.10371752345046958No Hit
TGAGGTAGTAGGTTGTATAGT96550.10054143462994815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)