FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6699422
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG90060913.443085090027171No Hit
CAACGGAATCCCAAAAGCAGCTG5525018.246995039273537No Hit
CCCCCACTGCTAAATTTGACTG2426983.62267073189299No Hit
AACATTCAACGCTGTCGGTGAGT2412173.600564347192937No Hit
AACATTCAACGCTGTCGGTGAG1539922.2985863556587423No Hit
TAGCAGCACGTAAATATTGGCG1423182.1243325170440075No Hit
TTCAAGTAATCCAGGATAGGCT1389402.073910256735581No Hit
TATTGCACTTGTCCCGGCCTGTAA1280661.911597746790693No Hit
AAGCTGCCAGTTGAAGAACTGT1275301.903597056581896No Hit
TATTGCACTTGTCCCGGCCTGTAT1182901.7656747104451698No Hit
TAGCTTATCAGACTGATGTTGACT730671.0906463273995877No Hit
TAGCTTATCAGACTGATGTTGAC694091.0360446020567147No Hit
TATTGCACTTGTCCCGGCCTGT625140.9331252755834758No Hit
TGAGGTAGTAGGTTGTATAGTT565720.8444310568881912No Hit
TATTGCACTTGTCCCGGCCTGTA564930.843251850682044No Hit
TCGTACGACTCTTAGCGG552490.8246830846004327No Hit
TGTAAACATCCTTGACTGGAAGCT467490.6978064674833142No Hit
TTAATGCTAATCGTGATAGGGGTT466550.6964033613646072No Hit
TAGCTTATCAGACTGATGTTGA424380.6334576326136792No Hit
AGCAGCATTGTACAGGGCTATGA420590.6278004281563394No Hit
ACTGGACTTGGAGTCAGAAGGC406660.6070075896099693No Hit
TGTAAACATCCCCGACTGGAAGCT366710.5473755795649237No Hit
TGAGGTAGTAGATTGTATAGTT359080.5359865373460576No Hit
CCCCCACTGCTAAATTTGACTGGCTT327300.48854960920509266No Hit
TTCTCACTACTGCACTTGACTAG314810.4699062098192949No Hit
CAACGGAATCCCAAAAGCAGCTGT313920.46857773700477445No Hit
TTCAAGTAATTCAGGATAGGTT292350.436380929578701No Hit
CACCCGGCTGTGTGCACATGTGC267850.3998106105272963No Hit
CTTCTCACTACTGCACTTGACTAG264730.3951534923460561No Hit
CATAAAGTAGAAAGCACTACT235860.35206022250874774No Hit
CCCCCCACTGCTAAATTTGACTGGCT209670.312967297775838No Hit
CCCCCCACTGCTAAATTTGACTGG208130.3106685920068925No Hit
CCCATAAAGTAGAAAGCACTAC196850.2938313185824091No Hit
CAACGGAATCCCAAAAGCAGCT196570.29341337207896445No Hit
CTCCCACTGCTTCACTTGACTAG187800.28032269052464526No Hit
AACATTCAACGCTGTCGGTGAGTT181550.27099352750132777No Hit
CAAAGAATTCTCCTTTTGGGCT169030.252305348133018No Hit
TATTGCACTTGTCCCGGCCTGTAG168730.2518575483078988No Hit
ACCCCCCACTGCTAAATTTGACTG165480.24700638353577367No Hit
CATTGCACTTGTCTCGGTCTGA161500.24106557252252508No Hit
TGAGAACTGAATTCCATGGGTT154810.23107963642236598No Hit
TGAGGTAGTAGTTTGTACAGTT149630.2233476261086404No Hit
CTCCCACTGCTTCACTTGACTAGCCT148520.2216907667556992No Hit
CCCCCCACTGCTAAATTTGACTGTA136440.20365936046423108No Hit
CGCCTGTCTGAGCGTCGCT134870.20131587471277373No Hit
CACTAGATTGTGAGCTCCTGGAA134500.20076358826179333No Hit
TATTGCACTTGTCCCGGCCTGTT134170.20027100845416215No Hit
CACTAGATTGTGAGCTCCTGGA125870.1878818799591965No Hit
CCCATAAAGTAGAAAGCACT125130.1867773070572357No Hit
TACGCCTGTCTGAGCGTCGCT118100.1762838644886081No Hit
GGAATACCGGGTGCTGTAGGCTTT116550.17397023205882536No Hit
ACTGGACTTGGAGTCAGAAGGCA116120.17332838564282113No Hit
AACGGAATCCCAAAAGCAGCTG115550.17247756597509456No Hit
TGTAAACATCCTACACTCTCAGCT114700.1712087998039234No Hit
TCGTTTCCCGGCCAATGCACCA114470.1708654866046653No Hit
TGAGAACTGAATTCCATAGGCTGT112390.16776074115050524No Hit
CAAAGAATTCTCCTTTTGGGCTT112240.1675368412379456No Hit
ACTGGACTTGGAGTCAGAAGGCT111070.16579042191998056No Hit
CCCCCCACTGCTAAATTTGACTGT109860.1639842959586663No Hit
TAGCTTATCAGACTGATGTTGACA107810.16092433048701815No Hit
TATTGCACTTGTCCCGGCCTGTTT106300.15867040470058463No Hit
CTACGCCTGTCTGAGCGTCGCT105050.1568045720959211No Hit
TGAGGTAGTAGTTTGTGCTGTT104060.1553268326730276No Hit
TATTGCACTTGTCCCGGCCTGTAAT103600.15464020627451144No Hit
TAGCAGCACGTAAATATTGGC103370.15429689307525338No Hit
TGTAAACATCCCCGACTGGAAGC99550.1485949086354017No Hit
TGTCAGTTTGTCAAATACCCCAA98930.14766945566348857No Hit
TCGTTTCCCGGGCGGCGCACCA98860.14756496903762742No Hit
TATTGCACTTGTCCCGGCCTGTAAA97990.14626634954478163No Hit
ACCGGGTGCTGTAGGCTTA95990.14328101737732002No Hit
ACCGGGTGCTGTAGGCTT95750.14292277751722463No Hit
GCCTGTCTGAGCGTCGCT94010.140325538531533No Hit
AGCTCGGTCTGAGGCCCCTCAGT93830.14005685863646147No Hit
CCCCCCACTGCTAAATTTGACTGA91600.13672821326974177No Hit
GGAATACCGGGTGCTGTAGGCTT82900.12374201834128377No Hit
TTCAAGTAATCCAGGATAGGCTT81850.12217471895336642No Hit
TGAGGTAGTAGGTTGTGTGGTT80900.12075668617382215No Hit
ATGCACCTGGGCAAGGATTCTGA80830.12065219954796101No Hit
TTAATGCTAATCGTGATAGGGGTTT79920.11929387341176596No Hit
AGCAGCATTGTACAGGGCTATGT78880.11774150068468592No Hit
ACCGGGTGCTGTAGGCTTT78550.11724892087705475No Hit
CAACACCAGTCGATGGGCTGT75450.11262165601748927No Hit
ATCCCGGACGAGCCCCCA75420.11257687603497735No Hit
CCCCCACTGCTAAATTTGACTGGCTA74510.11121854989878233No Hit
TGTCAGTTTGTCAAATACCCCA73750.11008412367514692No Hit
CCCCCACTGCTAAATTTGACTGGCT70820.10571061204981563No Hit
CTACGGGGATGATTTTACGAACTGAA70700.10553149211976795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)