FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8882759
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89321010.055546930857856No Hit
CAACGGAATCCCAAAAGCAGCTG8447019.50944408150666No Hit
AAGCTGCCAGTTGAAGAACTGT4232394.7647245636181275No Hit
AACATTCAACGCTGTCGGTGAGT4065584.576933810767578No Hit
AACATTCAACGCTGTCGGTGAG2794823.1463422569496706No Hit
CCCCCACTGCTAAATTTGACTG2636102.9676590347661125No Hit
TAGCAGCACGTAAATATTGGCG2449402.7574765903251457No Hit
TATTGCACTTGTCCCGGCCTGTAA2422702.7274183617950234No Hit
TTCAAGTAATCCAGGATAGGCT2045592.3028768426566564No Hit
TATTGCACTTGTCCCGGCCTGTAT2006732.25912917371731No Hit
TATTGCACTTGTCCCGGCCTGT1104571.2434987823040116No Hit
TGAGGTAGTAGGTTGTATAGTT945381.0642864452362155No Hit
TATTGCACTTGTCCCGGCCTGTA861870.9702728622942488No Hit
TAGCTTATCAGACTGATGTTGACT814070.916460752790884No Hit
TAGCTTATCAGACTGATGTTGAC804330.9054956911473113No Hit
AGCAGCATTGTACAGGGCTATGA670060.7543377007076292No Hit
GCCTGTCTGAGCGTCGCT665880.7496319555669585No Hit
ACTGGACTTGGAGTCAGAAGGC583790.6572169750412006No Hit
TGTAAACATCCCCGACTGGAAGCT572180.6441467116241699No Hit
TGTAAACATCCTTGACTGGAAGCT538970.6067596790591752No Hit
CAACGGAATCCCAAAAGCAGCTGT479890.5402488123340957No Hit
CACCCGGCTGTGTGCACATGTGC476280.5361847597126073No Hit
TAGCTTATCAGACTGATGTTGA436770.4917053361461231No Hit
TGAGGTAGTAGATTGTATAGTT419350.47209431213882985No Hit
CTCCCACTGCTTCACTTGACTAG318540.35860479835150316No Hit
CATTGCACTTGTCTCGGTCTGA303210.34134664691454536No Hit
TTCAAGTAATTCAGGATAGGTT295920.3331397373271075No Hit
CGCCTGTCTGAGCGTCGCT292100.32883927167223603No Hit
AACATTCAACGCTGTCGGTGAGTT288240.32449377496338694No Hit
TATTGCACTTGTCCCGGCCTGTAG273110.30746077879631767No Hit
CAACGGAATCCCAAAAGCAGCT261890.2948295681555697No Hit
TCGTACGACTCTTAGCGG238450.2684413705246309No Hit
CCCATAAAGTAGAAAGCACTAC238280.26824998854522564No Hit
ACTGGACTTGGAGTCAGAAGGCA237430.2672930786481993No Hit
TGAGGTAGTAGTTTGTGCTGTT225520.2538850823263358No Hit
TCCTGTCTGAGCGTCGCT207200.23326085960454404No Hit
TATTGCACTTGTCCCGGCCTGTT205090.23088547150721975No Hit
TGAGGTAGTAGTTTGTACAGTT204920.2306940895278145No Hit
AACGGAATCCCAAAAGCAGCTG200510.22572941582677183No Hit
TATTGCACTTGTCCCGGCCTGTAAT199000.22402949353911325No Hit
CATAAAGTAGAAAGCACTACT193050.21733112425992868No Hit
CAACACCAGTCGATGGGCTGT186980.21049766181881102No Hit
TGTCAGTTTGTCAAATACCCCAA177610.1999491374245322No Hit
ACCCCCCACTGCTAAATTTGACTG175720.19782142012408532No Hit
CACTAGATTGTGAGCTCCTGGAA171690.19328454143583093No Hit
TATTGCACTTGTCCCGGCCTGTAAA170440.19187732099902743No Hit
CACTAGATTGTGAGCTCCTGGA170150.19155084585768906No Hit
TCGTTTCCCGGCCAATGCACCA170020.19140449493226147No Hit
GGAATACCGGGTGCTGTAGGCTTT166880.18786955719501114No Hit
CAAAGAATTCTCCTTTTGGGCT166350.1872728957298065No Hit
CCCATAAAGTAGAAAGCACT163600.18417701076883883No Hit
TGTAAACATCCTACACTCTCAGCT158930.17891963521694104No Hit
CTCCCACTGCTTCACTTGACTAGCCT156290.1759475856544121No Hit
CCCCCCACTGCTAAATTTGACTGG155560.17512576891931889No Hit
ACTGGACTTGGAGTCAGAAGGCT154970.17446156087314763No Hit
TGTAAACATCCCCGACTGGAAGC153700.17303182490935531No Hit
CCCCCACTGCTAAATTTGACTGGCTT148210.16685131275091444No Hit
AACCGGGCGGAAACACCA147870.1664685487921039No Hit
TATTGCACTTGTCCCGGCCTGTTT141270.15903842488578154No Hit
CTTCTCACTACTGCACTTGACTAG135060.15204735375574188No Hit
ATGCACCTGGGCAAGGATTCTGA131920.14851241601849155No Hit
CCCCCCACTGCTAAATTTGACTGGCT130890.14735286637856548No Hit
CAAAGAATTCTCCTTTTGGGCTT127530.14357025784443775No Hit
CTACGCCTGTCTGAGCGTCGCT124050.13965255614837688No Hit
TAGCTTATCAGACTGATGTTGACA123740.1393035654800496No Hit
TAGCAGCACGTAAATATTGGC123480.1390108636291945No Hit
GGAATACCGGGTGCTGTAGGCTT122380.13777250964480744No Hit
TTCAAGTAATCCAGGATAGGCTT120480.13563353458086613No Hit
CAAAGTGCTGTTCGTGCAGGTAG117980.1328190937072592No Hit
TCGTTTCCCGGGCGGCGCACCA115190.12967817769231382No Hit
AACATTCAACGCTGTCGGTGA114500.12890139201119832No Hit
TACGCCTGTCTGAGCGTCGCT114320.1286987522682986No Hit
GCGTTCGCGCTTTCCCCTG113520.1277981311887444No Hit
ACCACAGGGTAGAACCACGGAC110550.12445457543089934No Hit
TTCTCACTACTGCACTTGACTAG109930.12375659409424482No Hit
AGCAGCATTGTACAGGGCTATGT109860.12367778974978383No Hit
ACTGGACTTGGAGTCAGAAGG107670.12121233954450414No Hit
TGAGGTAGTAGGTTGTGTGGTT105620.11890449802814643No Hit
TGATGTAGTAGGTTGTATAGTT101960.11478415658918587No Hit
AACATTCAACGCTGTCGGTGAGA101160.11388353550963165No Hit
TGTCAGTTTGTCAAATACCCCA97910.1102247623739426No Hit
AACATTCATTGCTGTCGGTGGGTT93580.10535015078085536No Hit
AGCTCGGTCTGAGGCCCCTCAGT91660.10318866018992523No Hit
TATTGCACTTGTCCCGGCCTGA90760.10217546147542672No Hit
AGCAGCATTGTACAGGGCTATG90050.10137616026732235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)