FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11075636
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG113442810.24255401676256No Hit
CCCCCCACTGCTAAATTTGACTG6795096.13516912256777No Hit
AACATTCAACGCTGTCGGTGAGT5261954.750923558701279No Hit
AAGCTGCCAGTTGAAGAACTGT4832994.363623000972585No Hit
TAGCAGCACGTAAATATTGGCG3638573.2852018610940266No Hit
TTCAAGTAATCCAGGATAGGCT3634463.2814910132474564No Hit
AACATTCAACGCTGTCGGTGAG3026942.7329717227976795No Hit
CCCCCACTGCTAAATTTGACTG2574182.3241825571010097No Hit
TATTGCACTTGTCCCGGCCTGTAA1978351.7862179652707981No Hit
TATTGCACTTGTCCCGGCCTGTAT1761461.5903917391290217No Hit
TATTGCACTTGTCCCGGCCTGT1502801.3568521031207599No Hit
TAGCTTATCAGACTGATGTTGAC1403901.267557005304255No Hit
TAGCTTATCAGACTGATGTTGACT1311751.1843563656299285No Hit
TGAGGTAGTAGGTTGTATAGTT1201111.084461425059473No Hit
TAGCTTATCAGACTGATGTTGA1065840.9623284838902254No Hit
CATAAAGTAGAAAGCACTACT986020.8902603877556106No Hit
TATTGCACTTGTCCCGGCCTGTA915780.8268419077694499No Hit
TCGTACGACTCTTAGCGG862240.7785015686683817No Hit
TGTAAACATCCTTGACTGGAAGCT845720.7635859466670808No Hit
AGCAGCATTGTACAGGGCTATGA725420.6549691593331525No Hit
CAACGGAATCCCAAAAGCAGCTGT682840.6165244144896058No Hit
ACTGGACTTGGAGTCAGAAGGC677900.6120641740122192No Hit
TGAGGTAGTAGATTGTATAGTT676970.6112244931126303No Hit
CACCCGGCTGTGTGCACATGTGC646410.5836323981755992No Hit
TGTAAACATCCCCGACTGGAAGCT624970.5642745933506663No Hit
CCCATAAAGTAGAAAGCACTAC622230.5618006947862858No Hit
TTCAAGTAATTCAGGATAGGTT610630.551327255608617No Hit
CAACGGAATCCCAAAAGCAGCT553300.4995649911210516No Hit
CCCATAAAGTAGAAAGCACT548190.49495126058675093No Hit
TTAATGCTAATCGTGATAGGGGTT531330.4797286584716219No Hit
CATTGCACTTGTCTCGGTCTGA438930.3963022981253627No Hit
CTCCCACTGCTTCACTTGACTAG432700.3906773389808043No Hit
ATCCCGGACGAGCCCCCA417300.37677294558976115No Hit
CGCCTGTCTGAGCGTCGCT401290.3623177937592026No Hit
AACATTCAACGCTGTCGGTGAGTT369020.3331817694261531No Hit
CAACACCAGTCGATGGGCTGT349940.3159547677442632No Hit
TGAGGTAGTAGTTTGTACAGTT333900.30147252943307273No Hit
TGTAAACATCCTACACTCTCAGCT313930.2834419621591031No Hit
AACGGAATCCCAAAAGCAGCTG295630.2669192089736427No Hit
TGTCAGTTTGTCAAATACCCCAA261600.23619411111018818No Hit
TGAGGTAGTAGTTTGTGCTGTT250590.22625337271828003No Hit
TATTGCACTTGTCCCGGCCTGTT250330.2260186232194702No Hit
TGAGAACTGAATTCCATGGGTT246060.22216331414286278No Hit
TATTGCACTTGTCCCGGCCTGTAG244270.22054715413182593No Hit
CACTAGATTGTGAGCTCCTGGA242790.2192108877539854No Hit
AGCTCGGTCTGAGGCCCCTCAGT240520.21716134405283813No Hit
TCGTTTCCCGGCCAATGCACCA238310.21516597331295467No Hit
TTCTCACTACTGCACTTGACTAG233680.2109856264687644No Hit
ACTGGACTTGGAGTCAGAAGGCA206010.18600286249927317No Hit
TAGCAGCACGTAAATATTGGC203980.18417001064318111No Hit
CACTAGATTGTGAGCTCCTGGAA199010.17968268368516266No Hit
TGAGGTAGTAGGTTGTGTGGTT196130.1770823815445No Hit
TGTAAACATCCCCGACTGGAAGC194200.17533981795718095No Hit
CAAAGAATTCTCCTTTTGGGCT193260.1744911082307147No Hit
ACCACAGGGTAGAACCACGGAC184430.16651865409805813No Hit
CCCCCACTGCTAAATTTGACTGGCTT183650.16581440560162866No Hit
CTTCTCACTACTGCACTTGACTAG177800.1605325418784077No Hit
TTCAAGTAATCCAGGATAGGCTT176770.15960257271004571No Hit
TAGCTTATCAGACTGATGTTGACA176510.1593678232112359No Hit
TCGTTTCCCGGGCGGCGCACCA174970.1579773838721316No Hit
TTCAAGTAATCCAGGATAGGC171560.15489855390697202No Hit
ACTGGACTTGGAGTCAGAAGGCT170670.15409498831489224No Hit
GCCTGTCTGAGCGTCGCT166580.15040219812207622No Hit
CAAAGAATTCTCCTTTTGGGCTT162890.14707056100435226No Hit
CTACGCCTGTCTGAGCGTCGCT162400.14662814848736452No Hit
CCCATAAAGTAGAAAGCACTA160580.14498490199569578No Hit
CAACGGAATCCCAAAAGCAGCTGA159740.14422648053800252No Hit
TTCACAGTGGCTAAGTTCCG159130.14367572209848717No Hit
TGTCAGTTTGTCAAATACCCCA158310.1429353582945485No Hit
CTCCCACTGCTTCACTTGACTAGCCT157740.14242071516254237No Hit
TATTGCACTTGTCCCGGCCTGTTT149010.1345385492986588No Hit
CCCCCCACTGCTAAATTTGACTGGCT148320.13391556024412504No Hit
ACTGGACTTGGAGTCAGAAGG146450.13222716961806977No Hit
AACATTCAACGCTGTCGGTGA145110.13101730681651147No Hit
TATTGCACTTGTCCCGGCCTGA144910.13083673027896547No Hit
CCCCCCACTGCTAAATTTGACTGG144840.13077352849082435No Hit
AGCAGCATTGTACAGGGCTATGT144210.13020471239755443No Hit
AACCGGGCGGAAACACCA142680.1288233018853274No Hit
TACGCCTGTCTGAGCGTCGCT141720.12795653450510652No Hit
ACCGGGTGCTGTAGGCTT134990.1218801340166831No Hit
CAAAGTGCTGTTCGTGCAGGTAG134200.12116685669337633No Hit
TTATAAAGCAATGAGACTGATT133560.1205890117732291No Hit
CAAAGTGCTGTTCGTGCAGGT133220.12028203165940086No Hit
TATTGCACTTGTCCCGGCCTGTAAT132080.11925274539538859No Hit
AACATTCAACGCTGTCGGTGAGA131580.11880130405152355No Hit
TTCACAGTGGCTAAGTTCCGC131250.11850335276457262No Hit
AATGACACGATCACTCCCGTTG130900.1181873438238671No Hit
ATCCCACCGCTGCCACCA127780.11537034983814924No Hit
TTCACAGTGGCTAAGTTCTGC126220.11396185284529031No Hit
TCAGTGCACTACAGAACTTTGT124860.11273393238997742No Hit
TGAGAACTGAATTCCATAGGCTGT124080.11202968389354796No Hit
AGCAGCATTGTACAGGGCTATG123070.11111777237894059No Hit
ACCCCCCACTGCTAAATTTGACTG121590.10978150600110008No Hit
TATTGCACTTGTCCCGGCCTGTAAA118050.1065853012865356No Hit
AATGCACCCGGGCAAGGATTCT114610.10347938484074413No Hit
TGTAAACATCCTACACTCAGCT113030.10205283019413061No Hit
AGAGGTAGTAGGTTGCATAGTT113020.10204380136725331No Hit
GGAATACCGGGTGCTGTAGGCTTT112870.10190836896409379No Hit
TGAGGTAGTAGGTTGTATAGT111060.10027415129930237No Hit
ACGCCTGTCTGAGCGTCGCT110790.10003037297361525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)