FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12414615
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG127807810.29494672206911No Hit
CCCCCCACTGCTAAATTTGACTG7590626.114261296061135No Hit
AACATTCAACGCTGTCGGTGAGT5776554.653023875488689No Hit
AAGCTGCCAGTTGAAGAACTGT5172234.166242771120973No Hit
TAGCAGCACGTAAATATTGGCG4540653.6575036761107773No Hit
TTCAAGTAATCCAGGATAGGCT4191413.376190079192951No Hit
AACATTCAACGCTGTCGGTGAG3507622.8253957130366105No Hit
CCCCCACTGCTAAATTTGACTG2700722.1754359680102846No Hit
TATTGCACTTGTCCCGGCCTGTAA2633422.1212256682949895No Hit
TAGCTTATCAGACTGATGTTGACT2247731.8105515152906475No Hit
TAGCTTATCAGACTGATGTTGAC2231781.7977037548083448No Hit
TATTGCACTTGTCCCGGCCTGTAT2209501.7797571652443511No Hit
TAGCTTATCAGACTGATGTTGA1474731.187898295678118No Hit
TATTGCACTTGTCCCGGCCTGT1437961.1582799788797318No Hit
TGAGGTAGTAGGTTGTATAGTT1253061.009342617551974No Hit
CATAAAGTAGAAAGCACTACT1208460.9734172183350027No Hit
TGTAAACATCCTTGACTGGAAGCT1199170.965934102668508No Hit
TATTGCACTTGTCCCGGCCTGTA1017180.8193407528143241No Hit
CCCATAAAGTAGAAAGCACTAC903600.7278518101447367No Hit
AGCAGCATTGTACAGGGCTATGA857960.6910886886141857No Hit
TGTAAACATCCCCGACTGGAAGCT828120.6670525022322481No Hit
CACCCGGCTGTGTGCACATGTGC787120.6340269110238215No Hit
TGAGGTAGTAGATTGTATAGTT754720.6079286389469186No Hit
CAACGGAATCCCAAAAGCAGCTGT751340.6052060414277849No Hit
TTCAAGTAATTCAGGATAGGTT748600.6029989653323925No Hit
ACTGGACTTGGAGTCAGAAGGC695090.5598965412942729No Hit
TTAATGCTAATCGTGATAGGGGTT656030.5284336244015622No Hit
CAACGGAATCCCAAAAGCAGCT612190.49312040687528363No Hit
CCCATAAAGTAGAAAGCACT546500.4402069657415877No Hit
TCGTACGACTCTTAGCGG514180.41417313384265236No Hit
CATTGCACTTGTCTCGGTCTGA471740.379987619430808No Hit
AACATTCAACGCTGTCGGTGAGTT432800.34862136280504874No Hit
TGTCAGTTTGTCAAATACCCCAA412500.3322696676457546No Hit
TGTAAACATCCTACACTCTCAGCT385150.3102391818030603No Hit
CAACACCAGTCGATGGGCTGT356780.2873870836912784No Hit
TGAGGTAGTAGTTTGTACAGTT340010.27387881138480735No Hit
TCGTTTCCCGGCCAATGCACCA326060.2626420553516964No Hit
TAGCTTATCAGACTGATGTTGACA319580.2574224009363158No Hit
CAAAGAATTCTCCTTTTGGGCT316510.25494950910680675No Hit
TATTGCACTTGTCCCGGCCTGTAG307090.24736167815111462No Hit
CGCCTGTCTGAGCGTCGCT301750.24306029627177322No Hit
TAGCAGCACGTAAATATTGGC280180.22568561328724251No Hit
CACTAGATTGTGAGCTCCTGGA275380.22181920260918284No Hit
CTCCCACTGCTTCACTTGACTAG274210.22087676500640577No Hit
AACGGAATCCCAAAAGCAGCTG271070.21834748802117507No Hit
TATTGCACTTGTCCCGGCCTGTT261200.21039718106441482No Hit
CAAAGAATTCTCCTTTTGGGCTT256040.20624078958550066No Hit
CACTAGATTGTGAGCTCCTGGAA249810.20122251072626898No Hit
ATCCCGGACGAGCCCCCA248660.20029618316798384No Hit
TGTCAGTTTGTCAAATACCCCA245560.19779912627173696No Hit
ACTGGACTTGGAGTCAGAAGGCA244300.1967841934687463No Hit
GCCTGTCTGAGCGTCGCT238310.19195923514341767No Hit
TGAGAACTGAATTCCATGGGTT235510.1897038289145495No Hit
TGTAAACATCCCCGACTGGAAGC230160.18539439201296215No Hit
TGAGGTAGTAGTTTGTGCTGTT229050.18450028454366085No Hit
TTCAAGTAATCCAGGATAGGCTT224500.18083524942175008No Hit
TGAGAACTGAATTCCATAGGCTGT202050.16275172447957506No Hit
TATTGCACTTGTCCCGGCCTGTAAT201630.16241341354524486No Hit
TGATGTAGTAGGTTGTATAGTT200530.1615273610981895No Hit
TTCACAGTGGCTAAGTTCCGC193260.15567135992537828No Hit
CCCATAAAGTAGAAAGCACTA192380.154962517967734No Hit
TTCTCACTACTGCACTTGACTAG191840.15452754676645228No Hit
TATTGCACTTGTCCCGGCCTGTAAA189590.1527151667611118No Hit
ACTGGACTTGGAGTCAGAAGGCT186790.15045976053224366No Hit
TTCAAGTAATCCAGGATAGGC180840.14566702229589884No Hit
TTATAAAGCAATGAGACTGATT179450.14454737420371072No Hit
ACCACAGGGTAGAACCACGGAC177490.142968589843503No Hit
AGCAGCATTGTACAGGGCTATGT176940.14252556361997534No Hit
CAAAGTGCTGTTCGTGCAGGTAG175490.14135758539431145No Hit
TATTGCACTTGTCCCGGCCTGTTT174170.14029432245784504No Hit
CTTCTCACTACTGCACTTGACTAG171090.13781337560609008No Hit
CAACGGAATCCCAAAAGCAGCTGA170490.13733007427133262No Hit
TCGTTTCCCGGGCGGCGCACCA168690.13588017026706026No Hit
ACCCCCCACTGCTAAATTTGACTG167710.13509077808695638No Hit
TTCACAGTGGCTAAGTTCTGC164360.13239234563456054No Hit
ATGCACCTGGGCAAGGATTCTGA161510.13009666429446262No Hit
CCCCCACTGCTAAATTTGACTGGCTT158570.12772848775415108No Hit
TTTAAACATCCTTGACTGGAAGCT156450.12602082303800805No Hit
TGAGGTAGTAGGTTGTGTGGTT154750.12465146925619522No Hit
TAGCAGCACGTAAATATTGGCGT148460.11958486026348784No Hit
TTCACAGTGGCTAAGTTCCG148270.11943181484081464No Hit
AGCTCGGTCTGAGGCCCCTCAGT147300.11865047768295674No Hit
CTCCCACTGCTTCACTTGACTAGCCT146600.11808662612573971No Hit
ATCACATTGCCAGGGATTTCC144010.11600037536403666No Hit
AACATTCAACGCTGTCGGTGA143450.11554929411826302No Hit
AACATTCAACGCTGTCGGTGAGA143100.11526736833965452No Hit
TAGCAGCACATCATGGTTTACA140660.11330194291164083No Hit
CACCCGTAGAACCGACCTTGCG139790.11260115597624251No Hit
ACTGGACTTGGAGTCAGAAGG139260.11217423979720677No Hit
ACTTGACTTGGAGTCAGAAGGC137550.110796830993148No Hit
TCAGTGCACTACAGAACTTTGT136830.11021686939143906No Hit
CTACGCCTGTCTGAGCGTCGCT135790.10937914707785945No Hit
CAAAGTGCTGTTCGTGCAGGT135640.10925832174417008No Hit
CCCCCCACTGCTAAATTTGACTGG134460.10830782911914708No Hit
TATTGCACTTGTCCCGGCCTGA132640.10684181507038276No Hit
TGTAAACATCCTACACTCAGCT131050.1055610665332755No Hit
TCCTGTCTGAGCGTCGCT127720.10287874412537158No Hit
CCCCCCACTGCTAAATTTGACTGGCT126190.10164632572174007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)